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Targeted genome modification of crop plants using a CRISPR-Cas system

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Figure 1: Genome editing in rice and wheat using an engineered type II CRISPR-Cas system.
Figure 2: HDR-mediated genome modification in rice protoplasts.

References

  1. Zhang, F. et al. Proc. Natl. Acad. Sci. USA 107, 12028–12033 (2010).

    Article  CAS  Google Scholar 

  2. Chen, K. & Gao, C. J. Genet. Genomics 40, 271–279 (2013).

    Article  Google Scholar 

  3. Mussolino, C. & Cathomen, T. Nat. Biotechnol. 31, 208–209 (2013).

    Article  CAS  Google Scholar 

  4. Barrangou, R. et al. Science 315, 1709–1712 (2007).

    Article  CAS  Google Scholar 

  5. Jinek, M. et al. Science 337, 816–821 (2012).

    Article  CAS  Google Scholar 

  6. Gasiunas, G., Barrangou, R., Horvath, P. & Siksnys, V. Proc. Natl. Acad. Sci. USA 109, E2579–E2586 (2012).

    Article  CAS  Google Scholar 

  7. Cong, L. et al. Science 339, 819–823 (2013).

    Article  CAS  Google Scholar 

  8. Jiang, W., Bikard, D., Cox, D., Zhang, F. & Marraffini, L.A. Nat. Biotechnol. 31, 233–239 (2013).

    Article  CAS  Google Scholar 

  9. Hwang, W.Y. et al. Nat. Biotechnol. 31, 227–229 (2013).

    Article  CAS  Google Scholar 

  10. Chang, N. et al. Cell Res. 23, 465–472 (2013).

    Article  CAS  Google Scholar 

  11. Wang, H. et al. Cell 153, 910–918 (2013).

    Article  CAS  Google Scholar 

  12. Shan, Q. et al. Mol. Plant 6, 1365–1368 (2013).

    Article  CAS  Google Scholar 

  13. Fu, Y. et al. Nat. Biotechnol. advance online publication, doi:10.1038/nbt.2623 (23 June 2013).

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (201263, 383601 and 31200273), the Ministry of Agriculture of China (2011ZX08002-004 and 2013ZX08010-002) and Chinese Academy of Sciences (KSCX2-EW-N-06, KSCX2-EW-J-6). We thank X. Wang for bioinformatics analysis.

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Correspondence to Jin-Long Qiu or Caixia Gao.

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Shan, Q., Wang, Y., Li, J. et al. Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol 31, 686–688 (2013). https://doi.org/10.1038/nbt.2650

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