Abstract
We describe here the development of a carbohydrate-based microarray to extend the scope of biomedical research on carbohydrate-mediated molecular recognition and anti-infection responses. We have demonstrated that microbial polysaccharides can be immobilized on a surface-modified glass slide without chemical conjugation. With this procedure, a large repertoire of microbial antigens (∼20,000 spots) can be patterned on a single micro-glass slide, reaching the capacity to include most common pathogens. Glycoconjugates of different structural characteristics are shown here to be applicable for microarray fabrication, extending the repertoires of diversity and complexity of carbohydrate microarrays. The printed microarrays can be air-dried and stably stored at room temperature for long periods of time. In addition, the system is highly sensitive, allowing simultaneous detection of a broad spectrum of antibody specificities with as little as a few microliters of serum specimen. Finally, the potential of carbohydrate microarrays is demonstrated by the discovery of previously undescribed cellular markers, Dex-Ids.
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Acknowledgements
We acknowledge the late Professor Elvin A. Kabat and his previous students, postdoctoral fellows, and collaborators for their contributions to the collection of carbohydrate antigens and antibodies that were applied in this study. This work is supported by research grants of NIH (AI45326) and Compass Pacific to D.W.
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Wang, D., Liu, S., Trummer, B. et al. Carbohydrate microarrays for the recognition of cross-reactive molecular markers of microbes and host cells. Nat Biotechnol 20, 275–281 (2002). https://doi.org/10.1038/nbt0302-275
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DOI: https://doi.org/10.1038/nbt0302-275
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