Letters

  • Letter |

    An optogenetic study shows that activation of pain-triggering sensory neurons induces an immune response.

    • Frédéric Michoud
    • , Corey Seehus
    • , Philipp Schönle
    • , Noé Brun
    • , Daniel Taub
    • , Zihe Zhang
    • , Aakanksha Jain
    • , Ivan Furfaro
    • , Outman Akouissi
    • , Rachel Moon
    • , Pascale Meier
    • , Katia Galan
    • , Benjamin Doyle
    • , Michael Tetreault
    • , Sébastien Talbot
    • , Liam E. Browne
    • , Qiuting Huang
    • , Clifford J. Woolf
    •  & Stéphanie P. Lacour
  • Letter |

    Testing sewage for the novel coronavirus reveals epidemiological trends.

    • Jordan Peccia
    • , Alessandro Zulli
    • , Doug E. Brackney
    • , Nathan D. Grubaugh
    • , Edward H. Kaplan
    • , Arnau Casanovas-Massana
    • , Albert I. Ko
    • , Amyn A. Malik
    • , Dennis Wang
    • , Mike Wang
    • , Joshua L. Warren
    • , Daniel M. Weinberger
    • , Wyatt Arnold
    •  & Saad B. Omer
  • Letter |

    SARS-CoV-2 is reliably detected in nasal swab samples using mass spectrometry and machine learning analysis.

    • Fabiane M. Nachtigall
    • , Alfredo Pereira
    • , Oleksandra S. Trofymchuk
    •  & Leonardo S. Santos
  • Letter |

    The activity of adenine or cytosine base editors at specific target nucleotides is predicted computationally.

    • Myungjae Song
    • , Hui Kwon Kim
    • , Sungtae Lee
    • , Younggwang Kim
    • , Sang-Yeon Seo
    • , Jinman Park
    • , Jae Woo Choi
    • , Hyewon Jang
    • , Jeong Hong Shin
    • , Seonwoo Min
    • , Zhejiu Quan
    • , Ji Hun Kim
    • , Hoon Chul Kang
    • , Sungroh Yoon
    •  & Hyongbum Henry Kim
  • Letter |

    A turnkey system allows for spatial profiling of proteins and RNA in fixed tissues, providing a window on cellular heterogeneity.

    • Christopher R. Merritt
    • , Giang T. Ong
    • , Sarah E. Church
    • , Kristi Barker
    • , Patrick Danaher
    • , Gary Geiss
    • , Margaret Hoang
    • , Jaemyeong Jung
    • , Yan Liang
    • , Jill McKay-Fleisch
    • , Karen Nguyen
    • , Zach Norgaard
    • , Kristina Sorg
    • , Isaac Sprague
    • , Charles Warren
    • , Sarah Warren
    • , Philippa J. Webster
    • , Zoey Zhou
    • , Daniel R. Zollinger
    • , Dwayne L. Dunaway
    • , Gordon B. Mills
    •  & Joseph M. Beechem
  • Letter |

    Viscoplastic electronic devices adapt as nerves enlarge in growing animals.

    • Yuxin Liu
    • , Jinxing Li
    • , Shang Song
    • , Jiheong Kang
    • , Yuchi Tsao
    • , Shucheng Chen
    • , Vittorio Mottini
    • , Kelly McConnell
    • , Wenhui Xu
    • , Yu-Qing Zheng
    • , Jeffrey B.-H. Tok
    • , Paul M. George
    •  & Zhenan Bao
  • Letter |

    SARS-CoV-2 in patient samples is detected in under an hour using a CRISPR-based lateral flow assay.

    • James P. Broughton
    • , Xianding Deng
    • , Guixia Yu
    • , Clare L. Fasching
    • , Venice Servellita
    • , Jasmeet Singh
    • , Xin Miao
    • , Jessica A. Streithorst
    • , Andrea Granados
    • , Alicia Sotomayor-Gonzalez
    • , Kelsey Zorn
    • , Allan Gopez
    • , Elaine Hsu
    • , Wei Gu
    • , Steve Miller
    • , Chao-Yang Pan
    • , Hugo Guevara
    • , Debra A. Wadford
    • , Janice S. Chen
    •  & Charles Y. Chiu
  • Letter |

    Millions of primary IgG-secreting cells from mouse and human are characterized for activity and antibody sequence at the single-cell level.

    • Annabelle Gérard
    • , Adam Woolfe
    • , Guillaume Mottet
    • , Marcel Reichen
    • , Carlos Castrillon
    • , Vera Menrath
    • , Sami Ellouze
    • , Adeline Poitou
    • , Raphaël Doineau
    • , Luis Briseno-Roa
    • , Pablo Canales-Herrerias
    • , Pascaline Mary
    • , Gregory Rose
    • , Charina Ortega
    • , Matthieu Delincé
    • , Sosthene Essono
    • , Bin Jia
    • , Bruno Iannascoli
    • , Odile Richard-Le Goff
    • , Roshan Kumar
    • , Samantha N. Stewart
    • , Yannick Pousse
    • , Bingqing Shen
    • , Kevin Grosselin
    • , Baptiste Saudemont
    • , Antoine Sautel-Caillé
    • , Alexei Godina
    • , Scott McNamara
    • , Klaus Eyer
    • , Gaël A. Millot
    • , Jean Baudry
    • , Patrick England
    • , Clément Nizak
    • , Allan Jensen
    • , Andrew D. Griffiths
    • , Pierre Bruhns
    •  & Colin Brenan
  • Letter |

    Single-cell CRISPR screens are readily multiplexed and scaled with an improved version of Perturb-seq.

    • Joseph M. Replogle
    • , Thomas M. Norman
    • , Albert Xu
    • , Jeffrey A. Hussmann
    • , Jin Chen
    • , J. Zachery Cogan
    • , Elliott J. Meer
    • , Jessica M. Terry
    • , Daniel P. Riordan
    • , Niranjan Srinivas
    • , Ian T. Fiddes
    • , Joseph G. Arthur
    • , Luigi J. Alvarado
    • , Katherine A. Pfeiffer
    • , Tarjei S. Mikkelsen
    • , Jonathan S. Weissman
    •  & Britt Adamson
  • Letter |

    Primary macrophages engineered to express chimeric antigen receptors have anti-tumor activity in humanized mice.

    • Michael Klichinsky
    • , Marco Ruella
    • , Olga Shestova
    • , Xueqing Maggie Lu
    • , Andrew Best
    • , Martha Zeeman
    • , Maggie Schmierer
    • , Konrad Gabrusiewicz
    • , Nicholas R. Anderson
    • , Nicholas E. Petty
    • , Katherine D. Cummins
    • , Feng Shen
    • , Xinhe Shan
    • , Kimberly Veliz
    • , Kristin Blouch
    • , Yumi Yashiro-Ohtani
    • , Saad S. Kenderian
    • , Miriam Y. Kim
    • , Roddy S. O’Connor
    • , Stephen R. Wallace
    • , Miroslaw S. Kozlowski
    • , Dylan M. Marchione
    • , Maksim Shestov
    • , Benjamin A. Garcia
    • , Carl H. June
    •  & Saar Gill
  • Letter |

    Pig embryos with a human endothelium are generated through blastocyst complementation using human induced pluripotent stem cells.

    • Satyabrata Das
    • , Naoko Koyano-Nakagawa
    • , Ohad Gafni
    • , Geunho Maeng
    • , Bhairab N. Singh
    • , Tara Rasmussen
    • , Xiaoyan Pan
    • , Kyung-Dal Choi
    • , Daniel Mickelson
    • , Wuming Gong
    • , Pruthvi Pota
    • , Cyprian V. Weaver
    • , Stefan Kren
    • , Jacob H. Hanna
    • , Demetris Yannopoulos
    • , Mary G. Garry
    •  & Daniel J. Garry
  • Letter |

    Anti-tumor activity of cytomegalovirus (CMV)-specific CD8 T cells is achieved by antibody-mediated delivery of CMV epitopes.

    • David G. Millar
    • , Rakesh R. Ramjiawan
    • , Kosuke Kawaguchi
    • , Nisha Gupta
    • , Jiang Chen
    • , Songfa Zhang
    • , Takashi Nojiri
    • , William W. Ho
    • , Shuichi Aoki
    • , Keehoon Jung
    • , Ivy Chen
    • , Feng Shi
    • , James M. Heather
    • , Kohei Shigeta
    • , Laura T. Morton
    • , Sean Sepulveda
    • , Li Wan
    • , Ricky Joseph
    • , Eleanor Minogue
    • , Ashok Khatri
    • , Aditya Bardia
    • , Leif W. Ellisen
    • , Ryan B. Corcoran
    • , Aaron N. Hata
    • , Sara I. Pai
    • , Rakesh K. Jain
    • , Dai Fukumura
    • , Dan G. Duda
    •  & Mark Cobbold
  • Letter |

    Point mutations, structural variants and DNA methylation at target loci are assessed by nanopore sequencing.

    • Timothy Gilpatrick
    • , Isac Lee
    • , James E. Graham
    • , Etienne Raimondeau
    • , Rebecca Bowen
    • , Andrew Heron
    • , Bradley Downs
    • , Saraswati Sukumar
    • , Fritz J Sedlazeck
    •  & Winston Timp
  • Letter |

    The targets of small-molecule drugs are detected in tissue and blood using thermal proteome assays.

    • Jessica Perrin
    • , Thilo Werner
    • , Nils Kurzawa
    • , Anna Rutkowska
    • , Dorothee D. Childs
    • , Mathias Kalxdorf
    • , Daniel Poeckel
    • , Eugenia Stonehouse
    • , Katrin Strohmer
    • , Bianca Heller
    • , Douglas W. Thomson
    • , Jana Krause
    • , Isabelle Becher
    • , H. Christian Eberl
    • , Johanna Vappiani
    • , Daniel C. Sevin
    • , Christina E. Rau
    • , Holger Franken
    • , Wolfgang Huber
    • , Maria Faelth-Savitski
    • , Mikhail M. Savitski
    • , Marcus Bantscheff
    •  & Giovanna Bergamini
  • Letter |

    Alignment of 1.4 million sequences is made possible by upgrading T-Coffee with a regressive alignment algorithm.

    • Edgar Garriga
    • , Paolo Di Tommaso
    • , Cedrik Magis
    • , Ionas Erb
    • , Leila Mansouri
    • , Athanasios Baltzis
    • , Hafid Laayouni
    • , Fyodor Kondrashov
    • , Evan Floden
    •  & Cedric Notredame
  • Letter |

    Expansions of short tandem repeats linked to neuropsychiatric disorders are measured by single-molecule sequencing.

    • Pay Giesselmann
    • , Björn Brändl
    • , Etienne Raimondeau
    • , Rebecca Bowen
    • , Christian Rohrandt
    • , Rashmi Tandon
    • , Helene Kretzmer
    • , Günter Assum
    • , Christina Galonska
    • , Reiner Siebert
    • , Ole Ammerpohl
    • , Andrew Heron
    • , Susanne A. Schneider
    • , Julia Ladewig
    • , Philipp Koch
    • , Bernhard M. Schuldt
    • , James E. Graham
    • , Alexander Meissner
    •  & Franz-Josef Müller
  • Letter |

    A type I CRISPR–Cas system, rather than the conventional type II CRISPR systems, is adapted for gene editing by fusing Cascade to the FokI nuclease domain.

    • Peter Cameron
    • , Mary M. Coons
    • , Sanne E. Klompe
    • , Alexandra M. Lied
    • , Stephen C. Smith
    • , Bastien Vidal
    • , Paul D. Donohoue
    • , Tomer Rotstein
    • , Bryan W. Kohrs
    • , David B. Nyer
    • , Rachel Kennedy
    • , Lynda M. Banh
    • , Carolyn Williams
    • , Mckenzi S. Toh
    • , Matthew J. Irby
    • , Leslie S. Edwards
    • , Chun-Han Lin
    • , Arthur L. G. Owen
    • , Tim Künne
    • , John van der Oost
    • , Stan J. J. Brouns
    • , Euan M. Slorach
    • , Chris K. Fuller
    • , Scott Gradia
    • , Steven B. Kanner
    • , Andrew P. May
    •  & Samuel H. Sternberg
  • Letter |

    Autoimmune diabetes in mice is averted by electrically stimulating the nerve projecting to the pancreas.

    • Mélanie Guyot
    • , Thomas Simon
    • , Franck Ceppo
    • , Clara Panzolini
    • , Alice Guyon
    • , Julien Lavergne
    • , Emilie Murris
    • , Douglas Daoudlarian
    • , Romain Brusini
    • , Hadi Zarif
    • , Sophie Abélanet
    • , Sandrine Hugues-Ascery
    • , Jean-Louis Divoux
    • , Stephen J. Lewis
    • , Arun Sridhar
    • , Nicolas Glaichenhaus
    •  & Philippe Blancou
  • Letter |

    A series of bright fluorescent RNAs with a broad emission spectra enable live cell imaging of RNA dynamics.

    • Xianjun Chen
    • , Dasheng Zhang
    • , Ni Su
    • , Bingkun Bao
    • , Xin Xie
    • , Fangting Zuo
    • , Lipeng Yang
    • , Hui Wang
    • , Li Jiang
    • , Qiuning Lin
    • , Mengyue Fang
    • , Ningfeng Li
    • , Xin Hua
    • , Zhengda Chen
    • , Chunyan Bao
    • , Jinjin Xu
    • , Wenli Du
    • , Lixin Zhang
    • , Yuzheng Zhao
    • , Linyong Zhu
    • , Joseph Loscalzo
    •  & Yi Yang
  • Letter |

    Preservation of human livers at subzero temperatures with ice-free supercooling extends ex vivo organ life.

    • Reinier J. de Vries
    • , Shannon N. Tessier
    • , Peony D. Banik
    • , Sonal Nagpal
    • , Stephanie E. J. Cronin
    • , Sinan Ozer
    • , Ehab O. A. Hafiz
    • , Thomas M. van Gulik
    • , Martin L. Yarmush
    • , James F. Markmann
    • , Mehmet Toner
    • , Heidi Yeh
    •  & Korkut Uygun
  • Letter |

    Mitochondrial DNA mutations in iPSCs can render them immunogenic.

    • Tobias Deuse
    • , Xiaomeng Hu
    • , Sean Agbor-Enoh
    • , Martina Koch
    • , Matthew H. Spitzer
    • , Alessia Gravina
    • , Malik Alawi
    • , Argit Marishta
    • , Bjoern Peters
    • , Zeynep Kosaloglu-Yalcin
    • , Yanqin Yang
    • , Raja Rajalingam
    • , Dong Wang
    • , Bjoern Nashan
    • , Rainer Kiefmann
    • , Hermann Reichenspurner
    • , Hannah Valantine
    • , Irving L. Weissman
    •  & Sonja Schrepfer
  • Letter |

    A general approach to mask antibodies using coiled-coil domains improves anti-tumor therapeutic efficacy.

    • Vivian H. Trang
    • , Xinqun Zhang
    • , Roma C. Yumul
    • , Weiping Zeng
    • , Ivan J. Stone
    • , Serena W. Wo
    • , Melissa M. Dominguez
    • , Julia H. Cochran
    • , Jessica K. Simmons
    • , Maureen C. Ryan
    • , Robert P. Lyon
    • , Peter D. Senter
    •  & Matthew R. Levengood