Articles

  • Article |

    A method to introduce defined mutations into the yeast genome enables saturation mutagenesis of a gene and genome-scale introduction of genetic variants.

    • Kevin R Roy
    • , Justin D Smith
    • , Sibylle C Vonesch
    • , Gen Lin
    • , Chelsea Szu Tu
    • , Alex R Lederer
    • , Angela Chu
    • , Sundari Suresh
    • , Michelle Nguyen
    • , Joe Horecka
    • , Ashutosh Tripathi
    • , Wallace T Burnett
    • , Maddison A Morgan
    • , Julia Schulz
    • , Kevin M Orsley
    • , Wu Wei
    • , Raeka S Aiyar
    • , Ronald W Davis
    • , Vytas A Bankaitis
    • , James E Haber
    • , Marc L Salit
    • , Robert P St.Onge
    •  & Lars M Steinmetz
  • Article |

    An engineered patch of retinal pigment epithelium generated from human embryonic stem cells is transplanted into the eyes of two patients.

    • Lyndon da Cruz
    • , Kate Fynes
    • , Odysseas Georgiadis
    • , Julie Kerby
    • , Yvonne H Luo
    • , Ahmad Ahmado
    • , Amanda Vernon
    • , Julie T Daniels
    • , Britta Nommiste
    • , Shazeen M Hasan
    • , Sakina B Gooljar
    • , Amanda-Jayne F Carr
    • , Anthony Vugler
    • , Conor M Ramsden
    • , Magda Bictash
    • , Mike Fenster
    • , Juliette Steer
    • , Tricia Harbinson
    • , Anna Wilbrey
    • , Adnan Tufail
    • , Gang Feng
    • , Mark Whitlock
    • , Anthony G Robson
    • , Graham E Holder
    • , Mandeep S Sagoo
    • , Peter T Loudon
    • , Paul Whiting
    •  & Peter J Coffey
  • Article |

    200 MB of digital data is stored in DNA, randomly accessed and recovered using an error-free approach.

    • Lee Organick
    • , Siena Dumas Ang
    • , Yuan-Jyue Chen
    • , Randolph Lopez
    • , Sergey Yekhanin
    • , Konstantin Makarychev
    • , Miklos Z Racz
    • , Govinda Kamath
    • , Parikshit Gopalan
    • , Bichlien Nguyen
    • , Christopher N Takahashi
    • , Sharon Newman
    • , Hsing-Yeh Parker
    • , Cyrus Rashtchian
    • , Kendall Stewart
    • , Gagan Gupta
    • , Robert Carlson
    • , John Mulligan
    • , Douglas Carmean
    • , Georg Seelig
    • , Luis Ceze
    •  & Karin Strauss
  • Article |

    Engineered switchgrass and poplar are better feedstocks for biofuel synthesis and yield more biomass in multi-year field trials.

    • Ajaya K Biswal
    • , Melani A Atmodjo
    • , Mi Li
    • , Holly L Baxter
    • , Chang Geun Yoo
    • , Yunqiao Pu
    • , Yi-Ching Lee
    • , Mitra Mazarei
    • , Ian M Black
    • , Ji-Yi Zhang
    • , Hema Ramanna
    • , Adam L Bray
    • , Zachary R King
    • , Peter R LaFayette
    • , Sivakumar Pattathil
    • , Bryon S Donohoe
    • , Sushree S Mohanty
    • , David Ryno
    • , Kelsey Yee
    • , Olivia A Thompson
    • , Miguel Rodriguez Jr.
    • , Alexandru Dumitrache
    • , Jace Natzke
    • , Kim Winkeler
    • , Cassandra Collins
    • , Xiaohan Yang
    • , Li Tan
    • , Robert W Sykes
    • , Erica L Gjersing
    • , Angela Ziebell
    • , Geoffrey B Turner
    • , Stephen R Decker
    • , Michael G Hahn
    • , Brian H Davison
    • , Michael K Udvardi
    • , Jonathan R Mielenz
    • , Mark F Davis
    • , Richard S Nelson
    • , Wayne A Parrott
    • , Arthur J Ragauskas
    • , C Neal Stewart Jr
    •  & Debra Mohnen
  • Article | | open

    A human genome is sequenced and assembled de novo using a pocket-sized nanopore device.

    • Miten Jain
    • , Sergey Koren
    • , Karen H Miga
    • , Josh Quick
    • , Arthur C Rand
    • , Thomas A Sasani
    • , John R Tyson
    • , Andrew D Beggs
    • , Alexander T Dilthey
    • , Ian T Fiddes
    • , Sunir Malla
    • , Hannah Marriott
    • , Tom Nieto
    • , Justin O'Grady
    • , Hugh E Olsen
    • , Brent S Pedersen
    • , Arang Rhie
    • , Hollian Richardson
    • , Aaron R Quinlan
    • , Terrance P Snutch
    • , Louise Tee
    • , Benedict Paten
    • , Adam M Phillippy
    • , Jared T Simpson
    • , Nicholas J Loman
    •  & Matthew Loose
  • Article |

    Combining gene activation and knockout of different genes in the same cell using two different Cas9 enzymes enables the reconstruction of directional dependency.

    • Michael Boettcher
    • , Ruilin Tian
    • , James A Blau
    • , Evan Markegard
    • , Ryan T Wagner
    • , David Wu
    • , Xiulei Mo
    • , Anne Biton
    • , Noah Zaitlen
    • , Haian Fu
    • , Frank McCormick
    • , Martin Kampmann
    •  & Michael T McManus
  • Article |

    Viruses are transduced to single cells in tissues, organoids and in the mouse brain using mechanical carriers.

    • Rajib Schubert
    • , Stuart Trenholm
    • , Kamill Balint
    • , Georg Kosche
    • , Cameron S Cowan
    • , Manuel A Mohr
    • , Martin Munz
    • , David Martinez-Martin
    • , Gotthold Fläschner
    • , Richard Newton
    • , Jacek Krol
    • , Brigitte Gross Scherf
    • , Keisuke Yonehara
    • , Adrian Wertz
    • , Aaron Ponti
    • , Alexander Ghanem
    • , Daniel Hillier
    • , Karl-Klaus Conzelmann
    • , Daniel J Müller
    •  & Botond Roska
  • Article |

    Combinatorial CRISPR screens are improved by using Cas9 enzymes from two different organisms.

    • Fadi J Najm
    • , Christine Strand
    • , Katherine F Donovan
    • , Mudra Hegde
    • , Kendall R Sanson
    • , Emma W Vaimberg
    • , Meagan E Sullender
    • , Ella Hartenian
    • , Zohra Kalani
    • , Nicolo Fusi
    • , Jennifer Listgarten
    • , Scott T Younger
    • , Bradley E Bernstein
    • , David E Root
    •  & John G Doench
  • Article |

    Droplet single-cell RNA-seq is applied to large numbers of pooled samples from unrelated individuals.

    • Hyun Min Kang
    • , Meena Subramaniam
    • , Sasha Targ
    • , Michelle Nguyen
    • , Lenka Maliskova
    • , Elizabeth McCarthy
    • , Eunice Wan
    • , Simon Wong
    • , Lauren Byrnes
    • , Cristina M Lanata
    • , Rachel E Gate
    • , Sara Mostafavi
    • , Alexander Marson
    • , Noah Zaitlen
    • , Lindsey A Criswell
    •  & Chun Jimmie Ye
  • Article |

    Efficient Cas9 genome editing in vivo is achieved without viral vectors using chemically modified single-guide RNAs.

    • Hao Yin
    • , Chun-Qing Song
    • , Sneha Suresh
    • , Qiongqiong Wu
    • , Stephen Walsh
    • , Luke Hyunsik Rhym
    • , Esther Mintzer
    • , Mehmet Fatih Bolukbasi
    • , Lihua Julie Zhu
    • , Kevin Kauffman
    • , Haiwei Mou
    • , Alicia Oberholzer
    • , Junmei Ding
    • , Suet-Yan Kwan
    • , Roman L Bogorad
    • , Timofei Zatsepin
    • , Victor Koteliansky
    • , Scot A Wolfe
    • , Wen Xue
    • , Robert Langer
    •  & Daniel G Anderson
  • Article |

    Protein origami using coiled-coil building blocks produces self-assembling polyhedral cages for diverse applications.

    • Ajasja Ljubetič
    • , Fabio Lapenta
    • , Helena Gradišar
    • , Igor Drobnak
    • , Jana Aupič
    • , Žiga Strmšek
    • , Duško Lainšček
    • , Iva Hafner-Bratkovič
    • , Andreja Majerle
    • , Nuša Krivec
    • , Mojca Benčina
    • , Tomaž Pisanski
    • , Tanja Ćirković Veličković
    • , Adam Round
    • , José María Carazo
    • , Roberto Melero
    •  & Roman Jerala
  • Article | | open

    Draft genome, 994 re-sequenced lines and GWAS for yield-traits provide a resource of genetics and genomics tools for pearl millet researchers and breeders.

    • Rajeev K Varshney
    • , Chengcheng Shi
    • , Mahendar Thudi
    • , Cedric Mariac
    • , Jason Wallace
    • , Peng Qi
    • , He Zhang
    • , Yusheng Zhao
    • , Xiyin Wang
    • , Abhishek Rathore
    • , Rakesh K Srivastava
    • , Annapurna Chitikineni
    • , Guangyi Fan
    • , Prasad Bajaj
    • , Somashekhar Punnuri
    • , S K Gupta
    • , Hao Wang
    • , Yong Jiang
    • , Marie Couderc
    • , Mohan A V S K Katta
    • , Dev R Paudel
    • , K D Mungra
    • , Wenbin Chen
    • , Karen R Harris-Shultz
    • , Vanika Garg
    • , Neetin Desai
    • , Dadakhalandar Doddamani
    • , Ndjido Ardo Kane
    • , Joann A Conner
    • , Arindam Ghatak
    • , Palak Chaturvedi
    • , Sabarinath Subramaniam
    • , Om Parkash Yadav
    • , Cécile Berthouly-Salazar
    • , Falalou Hamidou
    • , Jianping Wang
    • , Xinming Liang
    • , Jérémy Clotault
    • , Hari D Upadhyaya
    • , Philippe Cubry
    • , Bénédicte Rhoné
    • , Mame Codou Gueye
    • , Ramanjulu Sunkar
    • , Christian Dupuy
    • , Francesca Sparvoli
    • , Shifeng Cheng
    • , R S Mahala
    • , Bharat Singh
    • , Rattan S Yadav
    • , Eric Lyons
    • , Swapan K Datta
    • , C Tom Hash
    • , Katrien M Devos
    • , Edward Buckler
    • , Jeffrey L Bennetzen
    • , Andrew H Paterson
    • , Peggy Ozias-Akins
    • , Stefania Grando
    • , Jun Wang
    • , Trilochan Mohapatra
    • , Wolfram Weckwerth
    • , Jochen C Reif
    • , Xin Liu
    • , Yves Vigouroux
    •  & Xun Xu