Articles

  • Article |

    Combining gene activation and knockout of different genes in the same cell using two different Cas9 enzymes enables the reconstruction of directional dependency.

    • Michael Boettcher
    • , Ruilin Tian
    • , James A Blau
    • , Evan Markegard
    • , Ryan T Wagner
    • , David Wu
    • , Xiulei Mo
    • , Anne Biton
    • , Noah Zaitlen
    • , Haian Fu
    • , Frank McCormick
    • , Martin Kampmann
    •  & Michael T McManus
  • Article |

    Combinatorial CRISPR screens are improved by using Cas9 enzymes from two different organisms.

    • Fadi J Najm
    • , Christine Strand
    • , Katherine F Donovan
    • , Mudra Hegde
    • , Kendall R Sanson
    • , Emma W Vaimberg
    • , Meagan E Sullender
    • , Ella Hartenian
    • , Zohra Kalani
    • , Nicolo Fusi
    • , Jennifer Listgarten
    • , Scott T Younger
    • , Bradley E Bernstein
    • , David E Root
    •  & John G Doench
  • Article |

    Viruses are transduced to single cells in tissues, organoids and in the mouse brain using mechanical carriers.

    • Rajib Schubert
    • , Stuart Trenholm
    • , Kamill Balint
    • , Georg Kosche
    • , Cameron S Cowan
    • , Manuel A Mohr
    • , Martin Munz
    • , David Martinez-Martin
    • , Gotthold Fläschner
    • , Richard Newton
    • , Jacek Krol
    • , Brigitte Gross Scherf
    • , Keisuke Yonehara
    • , Adrian Wertz
    • , Aaron Ponti
    • , Alexander Ghanem
    • , Daniel Hillier
    • , Karl-Klaus Conzelmann
    • , Daniel J Müller
    •  & Botond Roska
  • Article |

    Droplet single-cell RNA-seq is applied to large numbers of pooled samples from unrelated individuals.

    • Hyun Min Kang
    • , Meena Subramaniam
    • , Sasha Targ
    • , Michelle Nguyen
    • , Lenka Maliskova
    • , Elizabeth McCarthy
    • , Eunice Wan
    • , Simon Wong
    • , Lauren Byrnes
    • , Cristina M Lanata
    • , Rachel E Gate
    • , Sara Mostafavi
    • , Alexander Marson
    • , Noah Zaitlen
    • , Lindsey A Criswell
    •  & Chun Jimmie Ye
  • Article |

    Efficient Cas9 genome editing in vivo is achieved without viral vectors using chemically modified single-guide RNAs.

    • Hao Yin
    • , Chun-Qing Song
    • , Sneha Suresh
    • , Qiongqiong Wu
    • , Stephen Walsh
    • , Luke Hyunsik Rhym
    • , Esther Mintzer
    • , Mehmet Fatih Bolukbasi
    • , Lihua Julie Zhu
    • , Kevin Kauffman
    • , Haiwei Mou
    • , Alicia Oberholzer
    • , Junmei Ding
    • , Suet-Yan Kwan
    • , Roman L Bogorad
    • , Timofei Zatsepin
    • , Victor Koteliansky
    • , Scot A Wolfe
    • , Wen Xue
    • , Robert Langer
    •  & Daniel G Anderson
  • Article |

    Protein origami using coiled-coil building blocks produces self-assembling polyhedral cages for diverse applications.

    • Ajasja Ljubetič
    • , Fabio Lapenta
    • , Helena Gradišar
    • , Igor Drobnak
    • , Jana Aupič
    • , Žiga Strmšek
    • , Duško Lainšček
    • , Iva Hafner-Bratkovič
    • , Andreja Majerle
    • , Nuša Krivec
    • , Mojca Benčina
    • , Tomaž Pisanski
    • , Tanja Ćirković Veličković
    • , Adam Round
    • , José María Carazo
    • , Roberto Melero
    •  & Roman Jerala
  • Article | | open

    Draft genome, 994 re-sequenced lines and GWAS for yield-traits provide a resource of genetics and genomics tools for pearl millet researchers and breeders.

    • Rajeev K Varshney
    • , Chengcheng Shi
    • , Mahendar Thudi
    • , Cedric Mariac
    • , Jason Wallace
    • , Peng Qi
    • , He Zhang
    • , Yusheng Zhao
    • , Xiyin Wang
    • , Abhishek Rathore
    • , Rakesh K Srivastava
    • , Annapurna Chitikineni
    • , Guangyi Fan
    • , Prasad Bajaj
    • , Somashekhar Punnuri
    • , S K Gupta
    • , Hao Wang
    • , Yong Jiang
    • , Marie Couderc
    • , Mohan A V S K Katta
    • , Dev R Paudel
    • , K D Mungra
    • , Wenbin Chen
    • , Karen R Harris-Shultz
    • , Vanika Garg
    • , Neetin Desai
    • , Dadakhalandar Doddamani
    • , Ndjido Ardo Kane
    • , Joann A Conner
    • , Arindam Ghatak
    • , Palak Chaturvedi
    • , Sabarinath Subramaniam
    • , Om Parkash Yadav
    • , Cécile Berthouly-Salazar
    • , Falalou Hamidou
    • , Jianping Wang
    • , Xinming Liang
    • , Jérémy Clotault
    • , Hari D Upadhyaya
    • , Philippe Cubry
    • , Bénédicte Rhoné
    • , Mame Codou Gueye
    • , Ramanjulu Sunkar
    • , Christian Dupuy
    • , Francesca Sparvoli
    • , Shifeng Cheng
    • , R S Mahala
    • , Bharat Singh
    • , Rattan S Yadav
    • , Eric Lyons
    • , Swapan K Datta
    • , C Tom Hash
    • , Katrien M Devos
    • , Edward Buckler
    • , Jeffrey L Bennetzen
    • , Andrew H Paterson
    • , Peggy Ozias-Akins
    • , Stefania Grando
    • , Jun Wang
    • , Trilochan Mohapatra
    • , Wolfram Weckwerth
    • , Jochen C Reif
    • , Xin Liu
    • , Yves Vigouroux
    •  & Xun Xu
  • Article |

    New computational tools reveal the contribution of microsatellite insertions and deletions to the mutational landscape in human cancer.

    • Yosef E Maruvka
    • , Kent W Mouw
    • , Rosa Karlic
    • , Prasanna Parasuraman
    • , Atanas Kamburov
    • , Paz Polak
    • , Nicholas J Haradhvala
    • , Julian M Hess
    • , Esther Rheinbay
    • , Yehuda Brody
    • , Amnon Koren
    • , Lior Z Braunstein
    • , Alan D'Andrea
    • , Michael S Lawrence
    • , Adam Bass
    • , Andre Bernards
    • , Franziska Michor
    •  & Gad Getz
  • Article |

    A scalable chemical method to control phosphorothioate chirality demonstrates its impact on the efficacy of antisense oligonucleotides.

    • Naoki Iwamoto
    • , David C D Butler
    • , Nenad Svrzikapa
    • , Susovan Mohapatra
    • , Ivan Zlatev
    • , Dinah W Y Sah
    • , Meena
    • , Stephany M Standley
    • , Genliang Lu
    • , Luciano H Apponi
    • , Maria Frank-Kamenetsky
    • , Jason Jingxin Zhang
    • , Chandra Vargeese
    •  & Gregory L Verdine
  • Article |

    Expansion microscopy, a technique for super-resolution imaging, is extended to clinical human tissue samples that are formalin fixed, paraffin embedded, stained and/or fresh frozen.

    • Yongxin Zhao
    • , Octavian Bucur
    • , Humayun Irshad
    • , Fei Chen
    • , Astrid Weins
    • , Andreea L Stancu
    • , Eun-Young Oh
    • , Marcello DiStasio
    • , Vanda Torous
    • , Benjamin Glass
    • , Isaac E Stillman
    • , Stuart J Schnitt
    • , Andrew H Beck
    •  & Edward S Boyden
  • Article |

    Longitudinal clinical and multi-omics data from 108 healthy individuals are analyzed to identify putative biomarkers and diagnostics of early disease states.

    • Nathan D Price
    • , Andrew T Magis
    • , John C Earls
    • , Gustavo Glusman
    • , Roie Levy
    • , Christopher Lausted
    • , Daniel T McDonald
    • , Ulrike Kusebauch
    • , Christopher L Moss
    • , Yong Zhou
    • , Shizhen Qin
    • , Robert L Moritz
    • , Kristin Brogaard
    • , Gilbert S Omenn
    • , Jennifer C Lovejoy
    •  & Leroy Hood
  • Article |

    Super-resolution imaging of the dynamics of organelle structures in live cells is facilitated by blinking, far-red dyes.

    • Hideo Takakura
    • , Yongdeng Zhang
    • , Roman S Erdmann
    • , Alexander D Thompson
    • , Yu Lin
    • , Brian McNellis
    • , Felix Rivera-Molina
    • , Shin-nosuke Uno
    • , Mako Kamiya
    • , Yasuteru Urano
    • , James E Rothman
    • , Joerg Bewersdorf
    • , Alanna Schepartz
    •  & Derek Toomre
  • Article |

    Lipid production in the oleaginous microalga Nannocholoropsis gaditana is doubled by decreasing the expression of a transcriptional regulator identified through a CRISPR–Cas9 reverse-genetics approach.

    • Imad Ajjawi
    • , John Verruto
    • , Moena Aqui
    • , Leah B Soriaga
    • , Jennifer Coppersmith
    • , Kathleen Kwok
    • , Luke Peach
    • , Elizabeth Orchard
    • , Ryan Kalb
    • , Weidong Xu
    • , Tom J Carlson
    • , Kristie Francis
    • , Katie Konigsfeld
    • , Judit Bartalis
    • , Andrew Schultz
    • , William Lambert
    • , Ariel S Schwartz
    • , Robert Brown
    •  & Eric R Moellering
  • Article |

    Detection of post-translational modifications by mass spectrometry is enhanced with an analytic method for data-independent acquisition proteomics.

    • George Rosenberger
    • , Yansheng Liu
    • , Hannes L Röst
    • , Christina Ludwig
    • , Alfonso Buil
    • , Ariel Bensimon
    • , Martin Soste
    • , Tim D Spector
    • , Emmanouil T Dermitzakis
    • , Ben C Collins
    • , Lars Malmström
    •  & Ruedi Aebersold
  • Article |

    Expression of the minimally polymorphic HLA-E molecule prevents NK-cell-mediated rejection of cells lacking expression of HLA-A, B and C.

    • Germán G Gornalusse
    • , Roli K Hirata
    • , Sarah E Funk
    • , Laura Riolobos
    • , Vanda S Lopes
    • , Gabriel Manske
    • , Donna Prunkard
    • , Aric G Colunga
    • , Laïla-Aïcha Hanafi
    • , Dennis O Clegg
    • , Cameron Turtle
    •  & David W Russell