CRISPR RNA is delivered to the mouse lung by inhalation using improved lipid nanoparticles.

We have designed a method, binding affinities to native chromatin by sequencing (BANC-seq), to determine the transcription factor concentrations required for binding to regulatory elements across the genome. Our study shows that chromatin context and DNA accessibility are key regulators of transcription factor binding.

Leveraging advances in hardware and probes, we have combined innovations in optics and algorithms to allow automated single-molecule-based super-resolution imaging at unprecedented throughput. This approach allows us to obtain nanoscale information from large cell populations, bridging the gap between imaging and indirect ensemble methods.

Fine-tuning gene expression is crucial for generating quantitative phenotypic changes and crop improvement. Using CRISPR–Cas base editing and prime editing, we engineered upstream open reading frames — eukaryotic translational control elements — in rice to incrementally downregulate translation to predictable and desired levels.