The Department of Neuroscience includes several research groups working on different aspects of nervous system function, both under normal conditions and in various disease states. Research projects include a broad range of studies on the molecular and cellular levels, networks of neurons, and the organization of the nervous system as a whole. The Department has a flat organization with 40 researchers, of which 24 are full professors and 65 PhD students. Teaching represents a fundamental activity at the Department, which participates in several undergraduate courses and postgraduate education programs. Each year approximately 15 students receive their PhD from our Department and many of our graduates have gone onto distinguished careers in the academic or private sector.
The main goal of our research is to study the mechanisms by which human neural stem cells balance between self-renewal and differentiation. We investigate cellular models of human neuroepithelial stem cells derived from iPS cells of patients with neurodevelopmental disorders or health controls. Using in vitro models we are investigating cellular and molecular mechanisms in addition to cellular signalling regulating normal and diseased behavior of the neural stem cells.
Description of the group
iPS core is a human cell facility, (www.ipscore.se), located at Karolinska Institutet in Solna. The facility was founded in 2013 by associate professor Anna Falk. The focus of the cell facility is culturing of human pluripotent stem cells, so called human induced pluripotent cells (iPS cells) and neuronal stem cells derived thereof. The services are aiming at deriving standardized cell cultures, cell culture advice and protocols for preclinical research.
Area of responsibilities/duties
Majority of the work will be advanced cell culturing (iPS cells and neural cells), and characterization of the generated cells. The work includes the whole reprogramming process from establishment of differentiated cells (for example fibroblast cells from skin biopsies) to reprogramming and expansion, characterisation and cryopreservation of established pluripotent stem cells cultures. Hands on cell culture demonstrations for researchers. Actively contribute to process development and standardization of existing and new processes. Contribute to a high standard of services and documentation.
Examples of specific duties:
Culture and cryopreservation of human iPS and rarely ES cells.
Derivation of human fibroblast cells from skin biopsies
Reprogramming of human fibroblast cells
Characterisation of iPS cells using immunocytochemistry, flow cytometry and quantitative RT-PCR.
Differention of stem cells to neuronal stem cells and other cell types.
Preparation of DNA and RNA
Ordering and inventory of medium and consumables
Documentation, electronic lab book
Medium preparation and factor aliquotation
Hand on cell culture training for researcher/ cell customers
Arrange with MTA-documents and cell shipments to customers
GMP cell production might be a part of the cell culture duties
The applicant should have an education in biology or equivalent.
Documented experience of culturing (with and without feeder cells) and cryopreservation of human ES and iPS cells is required.
Basic experience in molecular biology, immunocytochemistry, microscopy and flow cytometry is preferable. We have high demands on flexibility and accuracy since the stem cells are very sensitive.
Most of the work is advanced and labour intense cell culture with much routine and there is no opportunity to work on individual research projects.
Good ability to work in teams and to easily pick up new methods is a requirement. The applicant should be fluent in English, with good communication and writing skills since the working environment is international.
Personal qualities will be of great importance.
An overall assessment of the applicants’ qualifications will be made.
The application is to be submitted in Varbi recruitment system.
At Karolinska Institutet a 6-month trial period is required for all permanent appointments.
Karolinska Institutet is one of the world’s leading medical universities. Its vision is to significantly contribute to the improvement of human health. Karolinska Institutet accounts for the single largest share of all academic medical research conducted in Sweden and offers the country’s broadest range of education in medicine and health sciences. The Nobel Assembly at Karolinska Institutet selects the Nobel laureates in Physiology or Medicine.
Pursuant to the regulations of the Swedish National Archives, applications are kept on file for two years after the appointment has gained legal force. The regulations do not apply to attachments that have been printed or otherwise published.
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