The Institute of Molecular Biology gGmbH
funded by the Boehringer Ingelheim Foundation
2 Postdoc Positions available
1 Computational Biologist and 1 Molecular Biologist
non-coding RNA & R-loop epigenetics
The Institute of Molecular Biology gGmbH (IMB) is a Centre of Excellence for Life Sciences, funded by the Boehringer Ingelheim Foundation. It is located within the campus of the Johannes Gutenberg-University, Mainz, Germany.
The Niehrs laboratory studies regulation of DNA methylation, which plays important roles in development & disease. We have recently demonstrated a role for long non-coding RNAs and R-loop DNA:RNA hybrids in DNA methylation (Arab et al. 2014, Mol Cell, (2014) Nat. Genet. (2019)). In an ERC-funded project, we now aim to elucidate the mechanisms of DNA demethylation as well as the role played by long non-coding RNAs and R-loops in its regulation. See also www.imb-mainz.de/research-at-imb/niehrs/research/ Candidates will make use of mouse embryonic stem cells (ESCs) to interrogate genome-wide the role of ncRNAs and R-loops in targeting GADD45a and the DNA demethylation machinery in the genome. We will use CRISPR/CAS9 mediated ESC mutants, as well as ChIP-seq, RNA-seq, bisulfite-seq to carry out multi- dimensional data analysis to address: Where in the genome does demethylation occur? What are the molecular determinants in ncRNA & Rloops involved in targeting? What are the cofactors involved? The project will involve strong interaction between the Molecular- and the Computational Biologist.
- PhD & publications in a relevant field
- Sound experience in R scripting or Python (Computational Biologist)
- Sound experience in molecular biology (Molecular Biologist)
- Familiarity with Omics techniques or –analysis, or with ncRNAs or R-loops is advantageous
- Excellent communication skills and good team spirit with the ability to solve problems independently
- Fluent in English (spoken and written)
- An international and vibrant working environment
- Competitive salary
- Training opportunities
- Favourable pension scheme
To apply please send a single PDF file, stating job reference (#CNPD10), and containing your cover letter stating research and career interests, CV, scans of your degrees (Germans: including copies of Abitur- & Diplom/Master Zeugnis), and contact details of 2 references to firstname.lastname@example.org. Informal
enquires should be addressed to Prof. Christof Niehrs (email@example.com).
Starting date: before August 2019
Duration: Initially 3 years; option for further extension
Application deadline: 30th June 2019
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