Planning and Execution of Experiments. The employee will conduct and carry out research experiments and is required to discuss goals of projects with supervisors. Receives detailed verbal and/or written instructions on how to conduct an experiment in order to achieve the goals of the project. Verbally asks questions of supervisor and/or other laboratory personnel to clarify and obtain further information needed to prepare for and properly execute experiments. Clear and concise verbal communication is important. The employee must possess excellent reading (English) and writing skills; some editing experience in scientific writing is desirable. Receives verbal and/or written instructions on the operation and maintenance of all laboratory equipment. Position will require periodic communications with vendors and other laboratory personnel to obtain technical information by talking on the telephone. Develops a step-by-step written procedure outlining the specific actions required to conduct the experiment before proceeding. The successful candidate will exercise initiative and inventiveness in planning, executing and interpreting data obtained from experiments. Must be able to communicate results to principal investigators (PIs) and collaborators. Must have a well-developed esprit de corps and willingness to cooperate with all principals. Must have the ability to resolve, in consultation with PIs, various technical problems as they emerge that are related to experimental design and technique. The position will require detailed, legible handwritten records of all experiments performed.
Specific tasks: Sound working knowledge and trouble shooting skills in some of the following techniques: ELISA assays, ELISPOT assays, western blot analysis, immunoprecipitation, gene knockdown, transfection/transduction of mammalian /bacterial cells, proliferation/apoptosis study, gene expression library construction, RT-PCR, immunostaining of tissue sections, gel electrophoresis of proteins and nucleic acids, mammalian cell culture, T-cell isolation and culture, cryopreservation of cells, preparation of DNA, RNA, and protein from cells, blood, tissues, and FFPE slides for next-generation sequencing , microarray, methylation array, and protein array analyses. Data analyses from DNA/RNA/protein arrays and next-generation sequencing; Animal experiments including testing the efficacy and mechanisms of action of specific anti-cancer agents both in vivo and ex vivo according to IRB-approved animal protocols; establishment and maintenance of clinical databases under proper supervision.
Media and reagent preparation. Must be able to measure optical density by spectrophotometer and calculate sample concentrations. Must have a basic knowledge of solution and buffer preparation in which ionic strength and solution pH are critical for the proper execution of experiments. Should be able to maintain equipment such as ELISA plate reader, ELISPOT reader, high-speed centrifuges, and pH meters. Must have some knowledge of solvents, chemical characteristics, and uses based on previous experience. Must verbally or in writing report problems and/or possible problems with the operation of equipment to the supervisor and/or other faculty in the laboratory. The successful candidate must be able to accurately calculate quantities of chemicals required to prepare biological buffers, medias and experimental reagents (e.g. investigational drugs) of target concentrations (molarity). Must exercise proper caution when handling toxic, hazardous, flammable or potentially carcinogenic agents. Become familiar with Institutional regulatory requirements; and prepare for laboratory inspections
Ordering supplies and maintenance of equipment. Periodically reviews and place orders for laboratory supplies including community reagents and chemicals. Assist in assembly, operation, and maintenance of laboratory.
Tissue culture and handling of hazardous biological materials: Maintains and establishes tissue culture cell lines which requires good sterile techniques and the ability to observe and identify cell changes under the microscope, determine cell counts on the hemocytometer or coulter counter, perform colony counts and determine cell viability. Prepares sterile medias and solutions. Prepares cells for cryogenic storage and re-establishment by the use of small vials, dry ice and liquid nitrogen. The employee must have experience in sterile technique, the manipulation of viable cells and the re-feeding and characterization of cultures. Precaution against blood-borne pathogens must be taken whenever human-derived cells are used in accordance with the institutional Universal Precautions Plan. Must demonstrate and practice laboratory safety precautions. Must manually handle, store and dispose of radioactive isotopes, chemically hazardous materials, and biohazardous materials in procedures specified to meet MDACC safety guidelines. Accurately records use and disposal of all radioactive materials. The position requires the maintenance and analysis of various viable (human) cell lines established from human tumors, immortalized human cell lines (endothelial) and primary human cell lines (umbilical vein endothelial cells). Tests may periodically be performed for mycoplasma contamination. Requires use of single and multichannel pipettors. Fine finger dexterity will be required to manipulate small tissue culture vials.
Guide, teach, supervise, and assist students, collaborators, and laboratory members to accomplish research.
Other duties as assigned.
The above functions require a strong command of the English language, including spelling, writing, and the ability to clearly and accurately communicate verbally in English both face-to-face and over the phone.
- Employee Status: Regular
- Minimum Salary: US Dollar (USD) 39,200
- Midpoint Salary: US Dollar (USD) 49,000
- Maximum Salary : US Dollar (USD) 58,800