Senior Research Assistant - Lymphoma/Myeloma (Research)
MD Anderson Cancer Center
Houston, TX, United States
Posted about 5 days agoExpires on August 12, 2019
Senior Research Assistant - Lymphoma/Myeloma (Research)
Perform Laboratory Research
Assists principal investigator with scientific research projects. Functions in a separate and independent manner in the design and initiation of research experiments. Designs, develops or adapts equipment used in experiments or research to obtain desired results. Determines specific goals or objectives to be obtained, evaluates research data and develops and revises techniques or approaches to work problems. Represents department or research committees as needed. Plans, organizes, coordinates and participates in scientific research projects. Participates in research activities developing new and innovative techniques working with large numbers of materials and data, developing and implementing support systems to document research results. Works independently to see that samples are obtained and processed in a timely and consistent manner. Requires both an understanding of the methodology and the ability to independently troubleshoot problems and to ask for help when appropriate. •Molecular cloning: genomic, cDNA, and mutation library construction; protein expression and purification; Northern, Southern and Western blotting; chromatin and protein immunoprecipitation; yeast and mammalian two-hybrid analysis; semi-quantitative reverse transcriptase-PCR and real-time PCR; in vitro kinase activity assays. •Cell culture techniques: preparation of primary cultures from tissues, establishment of stable cell lines using retroviral or lentiviral transduction, plasmid transfection, knockdown, Tet-on and off systems, cDNA expression, microRNA, shRNA, and antagomir expression, drug IC50 titration and determination of synergy using combination index analysis, cell transfection, trans-well cell invasive and migration assays, clonogenicity assay, anoikis and autophagy assays, dual luciferase assays, and wound healing assays. •Immunological methods: monoclonal antibody preparation and genetic engineering of antibodies, immunofluorescence and immunohistochemistry, including tissue preparation, sectioning and immunostaining; immunofluorescence and confocal microscopy, as well as electron microscopy techniques, and ELISAs.
Under minimal supervision, manage genetically engineered mouse model colony. Perform mating, genotyping, weaning, euthanasia of undesirable genotypes, and overall colony management. Perform tumor implantation subcutaneously or surgically, dose animals via oral gavage, intraperitoneal and intravenous injection. Measure tumor response via caliper and non-invasive imaging methods. Perform mouse colonoscopy. Analyze the outcome of efficacy studies. Perform survival and non-survival animal surgeries for implantation of cell line and patient derive tumor fragments, collection of specimens for molecular characterization and ex vivo treatment. Drug dosing techniques (such as oral gavage, IP and SQ) are required. In vivo studies: animal experimentation, including making constructs for knockout, conditional knockout and drug-inducible and tissue specific transgenic mice, mouse ES cell culturing, positive clone screening and identification, mouse genotyping, nude mouse tumor modeling, including subcutaneous, intraperitoneal, and intravenous tumor and agent introduction, mouse handling and surgery using ACUF-approved protocols, and rabbit and rat handling.
Laboratory Operations and Management
Designs, develops or adapts equipment used in experiments or research to obtain desired results. Uses laboratory knowledge and expertise to identify and resolve laboratory operational issues and interacts with all levels of internal and external sources to resolve problems. Ensures laboratory adherence to safety standards. Supervise, coordinate, implement, and evaluate the various activities, functions, and procedures involving the daily activity within the research lab. Work closely with faculty and other laboratory members to ensure that no compromises in the daily functioning of the laboratory occurs due to lack of supplies or failure of equipment. Must be able to monitor lab inventory and estimate quantities and types of supplies needed in the lab. Maintain a clean and neat work area in laboratory to ensure the most efficient functioning of the lab and a pleasant work environment. Communicates results to and understands instructions from the principal investigator and colleagues. Compiles and writes project results. Maintains thorough, accurate, legible and organized handwritten records of all experiments
Education Required: Bachelor's degree in one of the natural sciences or related field.
Experience Required: Three years of experience in scientific or experimental research work. With preferred degree, one year of required experience.
It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. http://www.mdanderson.org/about-us/legal-and-policy/legal-statements/eeo-affirmative-action.html
Requisition ID: 126552
Employment Status: Full-Time
Employee Status: Regular
FLSA: exempt and not eligible for overtime pay
Work Week: Days
Fund Type: Soft
Pivotal Position: No
Minimum Salary: US Dollar (USD) 39,200
Midpoint Salary: US Dollar (USD) 49,000
Maximum Salary : US Dollar (USD) 58,800