St. Jude Children's Research Hospital (St. Jude)

Research Lab Specialist (Hematopoiesis and Leukemia research)

St. Jude Children's Research Hospital (St. Jude)

Memphis, TN, United States

Overview

The Division of Cancer Predisposition at St. Jude provides both cutting-edge clinical care and groundbreaking research to improve our understanding of and enhance the outcomes for children and families with hereditary forms of cancer or immunodeficiency. The Nichols Lab at St. Jude is seeking an industrious and independent Research Laboratory Specialist to conduct research in the field of cancer predisposition using in vitro and in vivo models of hematopoiesis and leukemia.
 
The lab is interested in identifying novel genes and genetic variants that contribute to development of cancer and primary immunodeficiency. In 2015, we and others identified germline variants in ETV6, the gene encoding the essential hematopoietic transcription factor ETV6, in families affected by autosomal dominant thrombocytopenia and predisposition to B-acute lymphoblastic leukemia (B-ALL). We are now using human and mouse models to decipher how these germline ETV6 variants impact hematopoiesis and promote leukemogenesis.
 
Towards this end, we have generated a knock-in mouse model harboring a heterozygous germline Etv6 variant that mimics a human variant observed in multiple kindreds. We are characterizing the hematopoietic phenotypes of these animals and examining the transcriptional mechanisms by which ETV6 regulates these processes. To model these cell compartments and perform assays in vitro, we also employ a number of cell lines including murine-derived HSPCs and pro-B cells, as well as human iPSCs. To study the role of ETV6 in malignancy, we have crossed the Etv6 variant mice with other mouse strains carrying known cooperating events identified in leukemia samples. Ultimately, our goal is to better understand how germline and somatic genetic events perturb key cellular and molecular pathways that then lead to leukemia.
 
Responsibilities
 

The Reserach Lab Specialist will have superb organizational and record keeping skills, attention to detail, ability to anticipate problems and offer solutions. He/she will assist the principal investigator with the collection and analysis of data for specified research projects. Performs laboratory experiments with minimal supervision, learning and developing new procedures. Manages specific, but limited, laboratory functions and trains laboratory personnel. This individual will also help conduct a wide array of cellular and molecular studies that span in vitro cell-based assays and in vivo murine assays for specified research projects.

In additon, the selected candidate will demonstrate effort to increase knowledge of a specific area of research and participates in the presentation and/or publication of research results. Properly conducts specific projects and maintains efficient laboratory operations.
 
Minimum Education
 
Bachelor’s degree in appropriate scientific field is required.

Preferred skills:

  • Experience working with mice, such as breeding, genotyping, completing flow cytometry, and carrying out retroviral or lentiviral transduction of bone marrow cells followed by adoptive transfer.
  • A background in hematopoiesis or leukemia modeling is desired, but not necessary.
  • Experience in handling and differentiating iPSC or a background in functional genomics, such as carrying out large scale mutagenesis assays.

Minimum Experience

  • A minimum of eight (8) years of relevant post-degree work experience, with at least five (5) years at the Sr. Research Tech level, is required with a Bachelor’s degree
  • A minimum of seven (7) years of relevant post-degree work experience, with at least (5) years at the Sr. Research Tech level, is required with a Master’s degree
  • A minimum of four (4) years of relevant work experience including post-doctoral and/or technical staff experience is required with a Ph.D
  • BS/MS staff at the Senior Research Technologist level as of 5/12/2003 could be considered for promotion to Research Lab Specialist after five (5) years as a SJCRH Senior Research Technologist or an equivalent position in a similar research environment.

EEO Statement

 

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Please apply via recruiter’s website.