Imperial College London (ICL)

Research Associate In Mitochondrial Microscopy

Imperial College London

London, United Kingdom

Research Associate In Mitochondrial Microscopy *
*£37,486 – £46,499 plus benefits

Applications are invited for a Research Associate In Mitochondrial Microscopy joint between the labs of Nick Jones at Imperial College London and Patrick Chinnery at the MRC Mitochondrial Biology Unit. Experiments will principally be undertaken in the Cambridge Mitochondrial Biology Unit.

Cells control their mitochondrial DNA (mtDNA) yet the process of this control has been poorly characterized. Through a combination of microscopy, image analysis and some flow-cytometry the appointee will attempt to understand and perturb this system. Beyond being of fundamental relevance to our biological understanding; the character of control of mtDNA defines the rate and manner in which mutant mtDNA expands through cellular populations. The expansion of mutant mtDNA is implicated in ageing: thus the project seeks to identify some of the basic mechanisms of ageing. The project is a close collaboration between two groups that both have a track record of working at the interface between experiment and theory and aligns with 2 other PDRAs and 2 PhDs joint between our groups.

Links for context:

The appointment will be split between Imperial and Cambridge and, like other current team members joint between the groups, there will be travel between the two sites throughout the appointment. It is anticipated that the appointment will be principally experimental and the experimental base will be in Cambridge.

Duties and responsibilities
• To develop/refine imaging tools (microscopy and associated image analysis) for counting mitochondrial nucleoids inside cells and linking them to measures of cell volume, mtDNA turnover and the mitochondrial network. This will be principally confocal but with possible super-resolution imaging/ electron microscopy.

• To develop imaging tools for tracking the amount of mitochondrial content through time.

• To complement these microscopy efforts with a pre-existing pipeline that links flow-cytometry linked to single-cell digital PCR for estimating mtDNA copy-number.

• To culture appropriate cell lines including monolayers.

• To engage with efforts (possibly by others in our team) to develop and refine stochastic models for the control of mitochondrial content and to use simpler statistical methods to explore the data.

• To engage with efforts (possibly by others in our team) to link the flow-cytometry data to CRISPR screens and single cell transcriptomics.

Essential requirements
• A PhD degree in Quantitative Cell Biology, Experimental Biological Physics, Biophysics, or closely aligned disciplines, or an equivalent level of professional qualifications.

Experience in:
• Quantitative Cellular Microscopy and Image Analysis

• Experience with basic cell culture

• Strong interest in the project

• Strong track record of high-quality independent research, commensurate with career stage

Experience and knowledge in one or more of the following techniques:
• Confocal microscopy

• Super-resolution microscopy

• Electron microscopy

• Single-cell video tracking

Further information
Candidates who have not yet been officially awarded their PhD will be appointed as Research Assistant. The salary range is £34,397 – £37,486 per annum.
This contract is full time, fixed term for 36 months.
Should you require any further details on the role please contact: Barsha Shrestha

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Closing date:
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