The University of Texas MD Anderson Cancer Center

Research Assistant II

MD Anderson Cancer Center

Houston, TX, United States

Research Assistant II JOB SPECIFIC COMPETENCIES Tissue Culture -Maintain and establish culture of human and murine cells, which require rigorous sterile techniques. Observe and identify cell changes under the microscope, determine cell counts and cell viability. -T cell culture and expansion. -Prepare sterile media and solutions. -Purify cells by various methods and monitor cells for proliferation and activation by standard laboratory methods. -Monitor supplies, order and store tissue culture supplies to ensure efficient functioning. -Prepare cells for cryogenic storage and keep them organized and updated on the laboratory inventoried cell stock list. Molecular and Cellular Biology -Protein, RNA, DNA isolation. -Polymerase chain reaction (PCR). -Plasmid transfection and transformation. -Tumor, and blood processing (mouse/human) and isolation of tumor/immune cells. -Flow cytometry and cell sorting. -Magnetic cell separation. -Functional assays: T cell / Tumor cell co-cultures, Cytokine assays (ELISA, ELISPOT) Work with Animals or Animal/Human Products -Conduct experiments involving animals and animal or human tissues, in accordance with appropriate institutional and regulatory guidelines. -Knowledgeable of safety concerns when working with animal/human tissues. -Work on animal tissues will require application of universal safety precautions at all times. -Effectively communicate with the Department of Veterinary Medicine to process samples (tissues, tissue blocks, and slides) and will manage/maintain accurate records/samples. Record Keeping and Data Collection -Maintain accurate, detailed records and experimental data and protocols. -Prepare graphs, slides and tables via computer. -Communicate results to and understand instructions from principal investigator and colleagues. Fluent spoken English is essential. -Discusses goals of each project with supervisor. Develop a step-by-step written procedure outlining the specific actions required to conduct the experiment before proceeding. -Read literature and confer with supervisor regarding new procedures and modify them according to the needs of the project. Laboratory Safety/Lab Equipment Maintenance -Demonstrate and practice lab safety precautions. -Receive verbal and/or written instructions from principal investigator on standards and specialized lab safety procedures. -Attend annual safety courses required by MDA and other agencies. Handle, store, and dispose of radioactive isotopes, chemically hazardous materials, and bio hazardous materials in procedure specified to meet MDA safety guidelines. -Follow manufacturer's guidelines regarding the handling and maintenance of all lab equipment. Have working knowledge of equipment operation manuals and procedure manuals whenever necessary. -Order reagents and materials when needed. -Monitor work flow as it pertains to the need for supplies, equipment maintenance, and preparing stock solutions. Organization and performance of laboratory procedures -Expand knowledge of ongoing techniques in the laboratory and enhance proficiency in planning, executing, and troubleshooting these techniques. -Participate in adopting new techniques (both in vitro and in vivo) while continuously increasing knowledge and skills to enhance lab research efforts. -Maintain assertiveness and flexibility in approaching new techniques and responsibilities; utilize good work habits and time management. -Maintain alertness and careful attention to avoid injury to self and others due to chemicals and hazardous materials in the laboratory. Other duties as assigned Education Required: Bachelor's degree with major course works in one of the basic sciences. Experience Required: Two years experience in scientific or experimental research work. No experience required with preferred degree. It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. sonj Additional Information Requisition ID: 128904 Employment Status: Full-Time Employee Status: Regular FLSA: exempt and not eligible for overtime pay Work Week: Days Fund Type: Soft Pivotal Position: Yes Minimum Salary: US Dollar (USD) 34,400 Midpoint Salary: US Dollar (USD) 43,000 Maximum Salary : US Dollar (USD) 51,600 Science Jobs: Yes

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Quote Reference: MD Anderson-md_anderson-4432_128904