At Boston Children’s Hospital, success is measured in patients treated, parents comforted, and teams taught. As a teaching hospital of Harvard Medical School, our reach is global, and our impact is profound. Join our acclaimed Division of Endocrinology and discover how your talents can change lives.
Our laboratory is using the principles of developmental biology to generate steroid-producing, adrenocortical organoids from human and mouse stem cells. First, we are employing human embryonic stem cells to generate organoids using high-throughput small molecule screening. Second, we are employing genetically engineered mouse models to generate organoids from adult adrenal stem cells. Our ultimate goal is to develop stem cell-based therapies for the treatment of patients living with adrenal insufficiency.
Boston Children’s Hospital cannot offer visa sponsorship for this opportunity.
This Research Assistant II will be responsible for:
- Prepare and maintain 3D organoid cultures derived from mouse or human stem cells.
- Dissect and process human and mouse tissues.
- Perform basic molecular biological and immunohistochemical techniques (RNA extraction, RT-qPCR, immunostaining, imaging, etc.)
- According to prescribed protocols and assigned schedules, performing a variety of routine laboratory experiments and procedures, utilizing standard laboratory equipment, recording and checking results for completeness and accuracy. Assisting in troubleshooting procedures if experimental results are unexpected or quality of specimens is poor.
- Handling, storage and disposal of bio-hazardous, flammable, corrosive and toxic materials. Carrying out all laboratory activities in compliance with applicable regulations and protocols.
- Conducting literature searches and reviews, compiling information for journals and suggesting uses for new techniques to the P.I. Attending courses, lectures and conferences to further own knowledge of research and laboratory techniques, consulting with other researchers and technicians to exchange information.
- Contributing to the team within and between departments and organizations.
- Assisting with planning specific research procedures, coordinating schedules of experiments and procedures. Developing and troubleshooting new techniques specific to the lab.
- Maintaining accurate records of results and methodologies into a log of work performed. Compiling and helping analyze data, preparing charts, graphs, and results of studies.
- Demonstrating basic laboratory techniques to laboratory assistants/technicians, research fellows, residents and students.
In order to qualify you must have:
- Bachelor’s degree, AND a minimum of one year of relevant work experience.
- The analytical skills to resolve complex problems requiring the use of scientific, mathematical, or technical principles and in-depth, experienced-based knowledge.
- The ability to communicate effectively both orally and in writing.
- Prior experience in stem cell biology (particularly with human embryonic stem cells or with induced pluripotent stem cells) is highly desired.
Boston Children’s Hospital offers competitive compensation and unmatched benefits.
Boston Children’s Hospital is an Equal Opportunity / Affirmative Action Employer. Qualified applicants will receive consideration for employment without regard to their race, color, religion, national origin, sex, sexual orientation, gender identity, protected veteran status or disability.