The University of Texas MD Anderson Cancer Center

Research Assistant II - Immunology

MD Anderson Cancer Center

Houston, TX, United States

This position is responsible for applying technical expertise and experience to plan and carry out laboratory and/or clinical research projects for the Imunotherapy Platform (IMT). Will perform under the supervision of the Group Leader of the IMP on processing, isolating, growing, and characterizing the phenotype and function of lymphocytes from patients taking part in clinical trials carried out at MD Anderson Cancer Center. KEY FUNCTIONS: 1. Tissue Culture: * Maintains and establishes tissue culture cell lines, which requires good sterile technique and ability to determine cell counts and cell viability using microscope and hemocytometer. * Monitors, orders and shelves tissue culture supplies. * Works with primary human leukocytes and sorts out the precious pDCs on a weekly basis. * Establishes short-term cell cultures using human or mouse tissues. * Some protocols require work on weekends/holidays and time away must be coordinated with other staff to ensure cell lines are maintained. * Requires cultivation of viruses; must be willing to work with infectious organisms. 2. Lab Functions: * Successfully completes training and certification by the Department of Veterinary Medicine to handle and work with live animals, primarily mice, in a safe and humane manner, following institutional guidelines for disposal of dead animals, biohazards and used needles. * Setting up breedings, transferring cages between rooms, ear tagging and tail snipping, and updating lab, breeding and genotyping records. * Performs Basic to intermediate techniques in immunological research, including in vivo (assays of delayed-type hypersensitivity, contact hypersensitivity) and in vitro measurements of immune function (mitogen and antigen-induced blastogenesis, ELISA, Western blotting, SDS-PAGE gels). * Requires a willingness to learn techniques for handling chemicals and low-level radioisotopes. * Requires the ability to calculate molarity and accurately prepare media solutions and reagents. * Uses acids and polyacrylamide solutions, flammable materials and other biohazards according to OSHA and institutional safety policies. * Discusses goals of projects and strategy for attaining the goals with supervisor in clear and concise manner. * Ensures safe and efficient functioning of the lab and pleasant work environment for self and others. * Responsible for ordering and maintaining laboratory supplies. 3. Lab Maintenance: * Responsible for ordering laboratory animals, and maintaining up-to-date animal inventory. * Maintains detailed written reports of experimental data and protocols. * Maintains lab work area and lab equipment, monitoring it daily to ensure the lab operates safely, is clean, and all equipment is in good working order; understands correct operation of all lab equipment, and follows written instructions/operations manuals. 4. Other duties as assigned. EDUCATION: Required: Bachelor's degree with major course works in one of the basic sciences. Preferred: Master's degree with major course works in one of the basic sciences. EXPERIENCE: Required: Two years experience in scientific or experimental research work. No experience required with preferred degree. Preferred: Must have wet lab experience. Willing to work with mice. It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. SONJ Additional Information Requisition ID: 127537 Employment Status: Full-Time Employee Status: Regular FLSA: exempt and not eligible for overtime pay Work Week: Days Fund Type: Soft Pivotal Position: Yes Minimum Salary: US Dollar (USD) 34,400 Midpoint Salary: US Dollar (USD) 43,000 Maximum Salary : US Dollar (USD) 51,600

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Quote Reference: MD Anderson-md_anderson-4432_127537