This position is responsible for applying technical expertise and experience to plan and carry out laboratory and/or clinical research projects for the Imunotherapy Platform (IMT). Will perform under the supervision of the Group Leader of the IMP on processing, isolating, growing, and characterizing the phenotype and function of lymphocytes from patients taking part in clinical trials carried out at MD Anderson Cancer Center.
1. Tissue Culture:
* Maintains and establishes tissue culture cell lines, which requires
good sterile technique and ability to determine cell counts and cell
viability using microscope and hemocytometer.
* Monitors, orders and shelves tissue culture supplies.
* Works with primary human leukocytes and sorts out the precious
pDCs on a weekly basis.
* Establishes short-term cell cultures using human or mouse tissues.
* Some protocols require work on weekends/holidays and time away must be coordinated with other staff to ensure cell lines are
* Requires cultivation of viruses; must be willing to work with
2. Lab Functions:
* Successfully completes training and certification by the Department of Veterinary Medicine to handle and work with live animals, primarily mice, in a safe and humane manner, following institutional guidelines
for disposal of dead animals, biohazards and used needles.
* Setting up breedings, transferring cages between rooms, ear tagging and tail snipping, and updating lab, breeding and genotyping records.
* Performs Basic to intermediate techniques in immunological
research, including in vivo (assays of delayed-type hypersensitivity,
contact hypersensitivity) and in vitro measurements of immune
function (mitogen and antigen-induced blastogenesis, ELISA, Western
blotting, SDS-PAGE gels).
* Requires a willingness to learn techniques for handling chemicals
and low-level radioisotopes.
* Requires the ability to calculate molarity and accurately prepare
media solutions and reagents.
* Uses acids and polyacrylamide solutions, flammable materials and
other biohazards according to OSHA and institutional safety policies.
* Discusses goals of projects and strategy for attaining the goals with
supervisor in clear and concise manner.
* Ensures safe and efficient functioning of the lab and pleasant work
environment for self and others.
* Responsible for ordering and maintaining laboratory supplies.
3. Lab Maintenance:
* Responsible for ordering laboratory animals, and maintaining
up-to-date animal inventory.
* Maintains detailed written reports of experimental data and
* Maintains lab work area and lab equipment, monitoring it daily to
ensure the lab operates safely, is clean, and all equipment is in good
working order; understands correct operation of all lab equipment, and follows
written instructions/operations manuals.
4. Other duties as assigned.
Required: Bachelor's degree with major course works in one of the basic sciences.
Preferred: Master's degree with major course works in one of the basic sciences.
Required: Two years experience in scientific or experimental research work. No experience required with preferred degree.
Preferred: Must have wet lab experience. Willing to work with mice.
It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. http://www.mdanderson.org/about-us/legal-and-policy/legal-statements/eeo-affirmative-action.html SONJ
Requisition ID: 127537
Employment Status: Full-Time
Employee Status: Regular
FLSA: exempt and not eligible for overtime pay
Work Week: Days
Fund Type: Soft
Pivotal Position: Yes
Minimum Salary: US Dollar (USD) 34,400
Midpoint Salary: US Dollar (USD) 43,000
Maximum Salary : US Dollar (USD) 51,600