The National Institute on Aging (NIA), a major research component of the National Institutes of Health (NIH) and the Department of Health and Human Services (DHHS), is recruiting for a postdoctoral position in The Section on Gene Targeting, Laboratory of Molecular Gerontology.
Replication of the genome is hindered by numerous impediments, including alternate DNA structures, protein DNA complexes, and a variety of chemical adducts, some from endogenous, others from exogenous, reactive agents. Failure to overcome these challenges can result in cell death, genomic rearrangements, and/or cellular senescence. Interstrand DNA crosslinks (ICLs) are blocks to replication and transcription, and crosslinking agents are frequently used in chemotherapy. We developed a DNA fiber based single molecule strategy for studying the encounters of replisomes with ICLs and discovered a new pathway that enables cells to continue DNA synthesis without resolving the ICLs (Mol. Cell 52, 434, 2013, Cell Reports 27, 6, 2019). We are now elucidating the mechanism of this “replication traverse” of the ICL and characterizing the requisite proteins and factors. We have discovered novel versions of the replisome that are associated with the block. Our findings have implications for DNA replication, the biology of cancer and aging, and genetic disorders in which the cellular response to replication stress is defective.
A postdoctoral position to participate in these studies is available starting in October/2019 for an individual with training in chemistry, biochemistry, or molecular and cellular biology, and an interest in the maintenance of genomic stability.
Interested candidates must have a Ph.D. or M.D. with less than five years of relevant postdoctoral experience. A background in DNA damage repair or replication stress is a plus. Experience in mammalian cell culture, DNA fiber assays/DNA combing, fluorescence or confocal microscopy and image analysis is desirable. Salary is commensurate with experience and accomplishments.
How to apply: Submit a cover letter describing your research experience and interests, CV including bibliography, and references (or contact information for 3 references) to:
Michael Seidman, Ph.D., Chief, Section on Gene Targeting, Laboratory of Molecular Gerontology, NIA-NIH, Laboratory of Molecular Gerontology, 251 Bayview Blvd, Baltimore, MD 21224 USA.
For additional information, visit https://www.nia.nih.gov/research/labs/lmg/section-gene-targeting
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