Francis Crick Institute

Postdoctoral Training Fellow - Retroviral Replication lab

Francis Crick Institute

London, United Kingdom

Salary for this Role:

From £38,300, subject to skills and experience


Job Title:

Postdoctoral Training Fellow – Retroviral Replication lab


Reports to:

Kate Bishop


Closing Date:

26/Jun/2022 23.59 GMT


Job Description:

Reporting to: Kate Bishop, Senior Group Leader


Contact term: This is a full-time, fixed term (4 years) position on Crick terms and conditions of employment.


Summary

Dr Bishop is seeking a talented and motivated postdoc to study the function of the HIV Vpr protein during HIV infection.


Many retroviruses have accessory proteins. These are dispensable for viral replication in cell culture but are required in vivo, and often increase transmission or pathogenicity in the host. In recent years, it has become clear that the function of retroviral accessory proteins is to block cellular proteins that interfere with viral replication. Therefore, studying viral accessory proteins not only allows us to discover more about how the virus replicates, but also to learn more about cellular defence mechanisms and general cell biology.


Viral protein R (Vpr) is a 14-16 kDa accessory protein found in primate lentiviruses like HIV-1, that is actively packaged into viral particles, and is thus present during both early and late stages of infection. Vpr is a paralogue of Vpx, an accessory protein found in HIV-2, that targets the cellular SAMHD1 protein for degradation. Like Vpx, Vpr also interacts with the DDB1/CUL4A/ROC1 E3 ubiquitin ligase complex through an interaction with the substrate-adaptor protein DCAF1, and it is assumed that it also degrades a cellular protein(s). Various targets for Vpr have been suggested, however, the role of Vpr during HIV infection is still currently unknown. Furthermore, numerous effects of Vpr on the cell have been reported over the years, including disruption of cell cycle control, enhancement of transcription, and induction of both DNA damage and apoptosis. Of all the proposed functions, the most well conserved phenotype of Vpr is its ability to cause cell cycle arrest at the G2/M boundary. Nonetheless, the purpose for this G2/M arrest phenotype remains unclear. Recently, Vpr has also been linked to silencing of integrated HIV-1 DNA that forms the viral reservoir in patients and is the main barrier to finding a cure for HIV.

The successful applicant will investigate the function and cellular targets of Vpr during HIV-1 replication.


The Research Group

Retroviruses cause severe diseases including AIDS and cancer. Many anti-retroviral dugs and cellular anti-viral factors target the early stages of the viral life cycle. However, numerous events that occur during these stages are still poorly understood. Dr Bishop’s laboratory focuses on the early, post-entry replication of retroviruses including HIV and MLV. She is interested in understanding the molecular events that occur when a retrovirus infects its target cell in order to fully understand retroviral replication and provide potential ways in which to manipulate these processes for the benfit of human health. Details of current research projects can be seen at:  https://www.crick.ac.uk/research/a-z-researchers/researchers-a-c/kate-bishop/. Her lab uses a wide range of research techniques including virological, biochemical and microscopy techniques and she collaborates closely with structural biologists.


Postdoctoral Training Fellows are expected to lead their own projects, contribute to other projects on a collaborative basis (both in the lab and with external collaborators) and guide PhD students in their research. The ability to work in a team is essential.


Key experience and competencies

The post holder should embody and demonstrate our core Crick values: bold, imaginative, open, dynamic and collegial, in addition to the following:


Essential

  • PhD in virology or molecular biology or in the final stages of PhD submission
  • Good knowledge and understanding of retrovirology
  • Expertise in molecular biology and biochemical techniques
  • Expertise in cell culture and transfection
  • Pro-active in innovation and problem solving
  • Experience of experimental design
  • Track record of writing papers as evidenced by publications or submitted manuscripts in referred journals
  • Evidence of data presentation at scientific meetings
  • Ability to work independently and also capable of interacting within a group


Desirable

  • Experience working with primary cells
  • Experience in flow cytometry and microscopy
  • Research experience of viral accessory proteins or innate immunity
  • A good understanding of DNA damage




Find out what benefits the Crick has to offer:

For more information on our great pay and benefits package please click here: https://www.crick.ac.uk/careers-and-study/life-at-the-crick/pay-and-benefits


Equality, Diversity & Inclusion:

We welcome applications from all backgrounds. We are committed to providing equal employment opportunities, regardless of ethnicity, nationality, gender, sexual orientation, gender identity, religion, pregnancy, age, disability, or civil partnership, marital or family status. We particularly welcome applications from people who are Minority Ethnic as they are currently underrepresented in the Crick at this level.

Diversity is essential to excellence in scientific endeavour. It increases breadth and perspective, leading to more innovation and creativity. We want the Crick to be a place where everyone feels valued and where diversity is celebrated and seen as part of the foundation for our Institute’s success.

The Crick is committed to creating equality of opportunity and promoting diversity and inclusivity. We all share in the responsibility to actively promote dignity, respect, inclusivity and equal treatment and it is our aim to ensure that these principles are reflected and implemented in all strategies, policies and practices.


Read more on our website: https://www.crick.ac.uk/careers-and-study/life-at-the-crick/equality-diversity-and-inclusion

Please apply via recruiter’s website.