Helmholtz Centre for Environmental Research - UFZ - UFZ - Helmholtz Association

Postdoctoral Researcher (m/f/d)

Max Delbrück Center for Molecular Medicine (MDC)

Berlin, Germany

The MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE (MDC) is a member of the Helmholtz Association of National Research Centers, supported by the Federal Government of Germany and the Land Berlin. For our Proteomics and Molecular Mechanisms of Neurodegenerative Diseases
 team, starting as soon as possible, we are looking for one

Postdoctoral Researcher (m/f/d)

The research group ‘Neuroproteomics’ of Prof. Erich Wanker at the Max Delbrück Center for Molecular Medicine in the Helmholtz Association is currently offering the position of Postdoctoral Researcher (m/f/d) with a focus on protein-protein-interaction research in Huntington’s disease.

The main objective of our work is to understand the pathomechanisms of neurodegenerative disorders such as Huntington’s and Alzheimer’s disease. One focus of our work lies on the analysis of protein-protein-interactions and their relevance to disease. To advance the possibilities of interactomics, we are constantly developing new detection approaches that enable high-throughput screening. After seminal studies using the Yeast-2-Hybrid system (Stelzl et al. 2005, Cell, https://doi.org/10.1016/j.cell.2005.08.029), we have established innovative methods to detect PPIs in mammalian cells with quantitative readouts, like a recent new assay: LuTHy (Trepte et al. 2018, Molecular Systems Biology, https://doi.org/10.15252/msb.20178071).

Currently, we are working on a large-scale effort to generate a detailed, quantitative interaction map for the full-length huntingtin protein that will comprise information on e.g. binding domains and affinities as well as on the impact of the Huntington’s disease mutation. To make interaction mapping more life-like, we are developing an approach in which interacting proteins can be measured endogenously in a cell. We employ CRSPR-based genome editing together with single- and multi-cell PPI techniques to advance the state of the art in binary interactomics and to unravel new disease biology. In particular, we want to answer questions like: Which known or newly detected PPIs of huntingtin can be confirmed in the cellular environment? Which interactors might be relevant targets for therapy? Does new quantitative information fundamentally change our view of huntingtin function?

Working in a highly motivated team of scientists and technicians, the successful candidate’s main task will be to systematically investigate the huntingtin interactome in its breadth and depth. Gaining a clear picture of the functional connections will be the main aim. Individual, highly promising interactions will be investigated in detail to determine their exact function in vivo and their value as targets for therapy. The effects of mutations on interactome networks and cellular processes will also be a central endeavor. Screenings, partly with automated systems, and detailed validation studies of newly discovered interactions and their role in disease mechanisms will be central to the project.


Description:

  • Mammalian-cell-based interaction screening using various methods like FRET/BRET, Co-IP
  • Interaction testing with the Yeast-2-Hybrid system
  • Classical and Gateway cloning
  • Yeast and mammalian cell culture
  • Genome engineering using CRISPR
  • Data analysis using bioinformatics tools
  • Managing collaborations
  • Visiting and presenting at project meetings and conferences

  • Writing reports, grant proposals and peer-reviewed publications
  • Mentoring of junior scientists, students and technicians
  • Managing milestone-driven projects

Requirements:

  • Doctoral degree in molecular biology, biochemistry, cell biology or other relevant discipline with a solid theoretical and experimental skills base in molecular biology, biochemistry, cell culture, proven by first-author publications
  • Bioinformatics skills
  • Experience with automated high-throughput screening technologies not essential but advantageous
  • High motivation to acquire and develop new techniques and workflows
  • High ambition to achieve, also with regard to writing papers and grant proposals; resilience, flexibility
  • Leadership skills and ability to work in a team
  • Independent, well-organized and reliable workstyle

We offer:

Stimulating, ambitious and nurturing academic environment in an internationally leading research group in network biology and Huntington’s disease
Comprehensive opportunities for your scientific and personal development at an internationally competitive and highly visible research center in Berlin, one of Europe’s most fascinating cities
State-of-the-art research facilities, including semi-automated and automated robotic systems for yeast-, DNA- and cell culture-based interaction studies
Collaboration with basic and clinical researchers locally, nationally and internationally
Travel to prestigious conferences
Full-time position
The initial contract will be until August 31, 2021.

Employment, compensation and benefits governed by the collective agreement for the public service in Germany (TVöD – Bund), postdoctoral salary group E13, level according to personal qualifications.

The MDC is an equal opportunity employer and promotes gender equality. The MDC is committed to raising the proportion of women in science and technology; we thus strongly encourage female researchers and specialists to apply.


MDC postdoctoral researchers benefit from the professional developmental program, which aims to enhance research experiences and career opportunities.


For more information click https://www.mdc-berlin.de/de/content/beruf-und-familie

For further information please contact:

Prof. Wanker, ewanker@mdc-berlin.de (EXCLUSIVELY per e-mail)

Please include in you application: Cover letter outlining your motivation and research profile, skills and relevant previous experience. Please let us know if you might be eligible for international postdoc fellowships like EMBO, MCSF, Humboldt etc.

Full CV with publications, awards and method list including all techniques you have previously acquired and used; please indicate your level of routine.

Names and contact details of two mentors who we may ask for references.

Please, send your application including all documents indicating the registration number 11057/2019 until May 20, 2019 preferably by e-mail as pdf files (2 files of 5 MB at the maximum) as we do not return any papers to:

MAX DELBRÜCK CENTRUM FOR MOLECULAR MEDICINE
Personnel Department • POB 74 02 38 • 13092 Berlin
jobs@mdc-berlin.dewww.mdc-berlin.de

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Position

Postdoctoral Researcher (m/f/d)