A postdoctoral position is open immediately in the laboratory of Dr. Jason M. Held at the Washington University School of Medicine in St. Louis, MO. The Held Laboratory focuses on redox systems biology and signaling in cancer and is especially interested in the redox regulation of cysteines. This interdisciplinary position is focused on understanding the mechanistic role of NADPH oxidase- and mitochondrial-derived reactive oxygen species (ROS) in cellular transformation, migration, cell polarity and signaling in breast and lung cancer models. It will also utilize optogenetics to precisely control the space and time of intracellular ROS production in order to systematically explore redox signaling networks activated by receptor tyrosine kinases and mitochondria. Techniques include cell-based and in vivo assays, DNA cloning, CRISPR/Cas9, and functional studies as well chemical biology, proteomics, and optogenetics.
- Redox Systems Biology: Harnessing the Sentinels of the Cysteine Redoxome (Preprint)
- Spatial and Temporal Alterations in Protein Structure by EGF Regulate Cryptic Cysteine Oxidation (Preprint)
Link to the Held Lab website for a full list of publications and further information. We have a longstanding interest in applying new biochemical and informatic technologies to biological models.
- Wang X, et al. (2017) Breast tumors educate the proteome of stromal tissue in an individualized but coordinated manner. Science Signaling 10(491). doi:10.1126/scisignal.aam8065. (PMID: 28790197)
- Held JM, et al. (2010) Targeted Quantitation of Site-Specific Cysteine Oxidation in Endogenous Proteins Using a Differential Alkylation and Multiple Reaction Monitoring Mass Spectrometry Approach. Molecular & Cellular Proteomics 9(7):1400–1410. (PMID: 20233844)
Expertise in the following area(s) is preferred:
- In vivo and in vitro cancer assays as well as rodent biology.
- Molecular biology techniques, including CRISPR/Cas9 and viral transduction of human cells.
- Prior experience in optogenetics, redox biology, LC-MS based proteomics and chemical biology isn’t required, but an interest in pursuing these techniques is essential.
A PhD., M.D. or equivalent terminal or doctoral degree in cancer biology, redox biology, NADPH oxidases (NOX), mitochondria and/or redox signaling.
Information about Washington University:
Consistently ranked among the top 10 US medical schools, Washington University School of Medicine offers a highly interactive and stimulating academic environment for scientists in training. The Siteman Cancer Center at Washington University is an NCI-designated Comprehensive Cancer Center. We are located in the heart of the Central West End, a vibrant St. Louis neighborhood adjacent to major cultural institutions and one of the country’s largest urban parks. We offer competitive salary and benefit packages. Links for more information on Washington University and St. Louis.
To Apply: Please send CV, brief cover letter stating research interests, and contact information of 3 references to Dr. Jason Held at jheld [at] wustl [dot] edu.
Keywords: Systems Biology, Proteomics