Department of Health and Human Services National Institutes of Health National Institute on Aging/National Cancer Institute Intramural Research Program
The National Institute on Aging (NIA), Intramural Research Program (IRP), and the National Cancer Institute (NCI), Center for Cancer Research (CCR), both major research components of the National Institutes of Health (NIH) and the Department of Health and Human Services (DHHS), are recruiting for a Postdoctoral Fellow in the Longitudinal Studies Sections (LSS) of the Translational Gerontology Branch (TGB) of NIA. This position is a joint appointment, funded by the NIA, and will be located in Bethesda, MD during the first period of fellowship and subsequently in Baltimore, MD for the remainder of the fellowship.
The NIA IRP TGB LSS, under the leadership of Luigi Ferrucci, M.D., Ph.D., focuses on mechanisms underlying heterogeneity in human health and function with aging, including molecular, cellular, physiologic, and behavioral factors. In addition to their longitudinal studies of aging, including the Baltimore Longitudinal Study of Aging (BLSA) and Genetic and Epigenetic Signatures of Translational Aging Laboratory Testing (GESTALT), they carry out smaller human studies of mechanisms and collaborate with other laboratories to pursue translational aging research across species.
The NCI CCR RNA Processing in Cellular Development Section, under the leadership of Shalini Oberdoerffer, Ph.D., broadly examines how DNA and RNA epigenetics dynamically regulate gene expression. They ask how methylation of cytosine in DNA affects pre-mRNA splicing decisions (Shukla et al., Nature, 2011; Marina et al., EMBOJ, 2016), and how subsequent acetylation of cytidine in processed mRNA affects translation (Arango et al., Cell, 2018). They use a variety of tools to investigate the enzymatic regulation of cytosine modifications and the net impact at target genes and genome-wide. Current efforts are focused on developing physiologically relevant model systems that interrogate how the epigenome and epitranscriptome are modulated in response to environmental cues to dynamically regulate cellular proteomes.
The position will build on the respective expertise of the two laboratories to examine how mRNA processing and function is perturbed in normal cellular aging. Specifically, the mechanistic bases supporting aberrant pre-mRNA splicing and mRNA translation in aged tissues will be assessed, with a specific focus on altered mRNA modifications. The successful applicant will have access to a wealth of aging relevant cell lines and primary tissues, as well as state-of-the art technologies to profile the transcriptome and proteome. Anticipated methodologies include transcriptome-wide analysis of pre-mRNA splicing and mapping of modified nucleotides, CRISPR/Cas9-guided mutation of involved factors, protein and nucleotide mass spectrometry, and in vitro approaches for validation.
The candidate will join an integrated team of bench and computational scientists spanning the two campuses. While candidates with a wide range of expertise will be considered, applicants with a background in RNA biology and/or computational biology are especially encouraged to apply. Expertise in the analysis of RNA-sequencing data, particularly from single cell and/or long-read single molecule (nanopore) data, is especially preferred. The fellow will be mentored jointly by the two laboratories and will lead this collaborative project under the mentorship of Dr. Ferrucci (NIA) and Dr. Oberdoerffer (NCI).
Eligible candidates must possess a recent Ph.D. or equivalent in the Biomedical Sciences by the time of selection and have no more than two years of post-doctoral experience. Successful applicants should have a strong background in gene regulation and cell biology. Additional information regarding the National Institute on Aging and the National Cancer Institute is available at: www.irp.nia.nih.gov and http://ccr.cancer.gov, respectively.
To apply, please send a curriculum vitae and bibliography, a brief summary of research interests (1-2 pages), and the contact information of three references to: Sarah Lewis, Intramural Program Specialist; Office of the Scientific Director, National Institute on Aging; Vacancy #NIA-IRP-21-01; via email: email@example.com AND firstname.lastname@example.org. Applications must reference the Vacancy #NIA-IRP-21-01. The first round of reviews is expected to occur on or about February 8, 2021 ; however, applications will be accepted until the position is filled.
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