The Post-Doctoral Associate is responsible for performing research activities and experiments in the laboratory on several aspects of cancer biology with a focus on melanoma. Several projects are ongoing in the laboratory including: 1) deciphering the role of the tumor microenvironment, specifically of immune responses triggered by the tumor; 2) understanding the role of Notch and ERBB signaling in melanoma growth and progression; 3) understanding the role of metalloproteinases in DNA repair processes; 4) understanding the role of obesity in melanoma development. The principle models used in the laboratory include a vast array of cancer cell lines, human tumor tissues and mouse models. Projects will entail manipulation of gene expression using both expression constructs, RNAi, CRISPR/cas9 in conjunction with cell culture, biochemical and in vivo assays (orthotopic, xenograft and transgenic mouse models). The ideal candidate should be highly motivated, have a PhD or PhD/MD degree, a strong background in cancer immunology, molecular biology, cell biology, mouse genetics, metabolism and have published in internationally recognized journals. For this position, an expertise in cancer immunology and an understanding of cancer metabolism is a plus. The incumbent will assist the Principal Investigator and ensure that all experiments are appropriately conducted following the policies and procedures of the University of Miami. This position will require advance knowledge and capability to exercise independent judgement when carrying out research activities and performing non-routine and complex laboratory projects.
DUTIES & RESPONSIBILITIES:
- Perform complex laboratory assignments, including the planning and conducting of projects related to cell culture and molecular biology. Schedule and perform experiments in research laboratory.
- Solve problems in scientific and technical execution, interpret experimental data and maintain accurate records. May need to develop, adapt and implement new lab procedures.
- Maintain accurate records of research findings and the analysis of the results.
- Assist in the preparation of data and figures for manuscripts and for scientific presentations using Power Point, and other graphics software as needed.
- Search literature for references to technical problems and keep informed by reading technical journal and publications.
- Present data in laboratory meetings and other venues.
- Prepare manuscripts for publication in collaboration with UM Faculty and staff.
- Ensure and document laboratory adherence to safety standards, especially in the use of hazardous items.
- Assist with the implementation of new program and initiatives.
- Mentor graduate and undergraduate students as appropriate.
Knowledge, Skills, and Abilities specific to the projects:
- Ability to perform basic cell and molecular biology techniques (cell culture of immortalized, cancer and normal cells; preparation of normal cells from animal tissues; analysis of immune cels by FACS; functional assays for immune cells; DNA and RNA extraction; PCR, RT-PCR; survival in vitro assays; viability assay; western blotting).
- Abilities to handle small animals including tail tipping for genotyping. Abilities to inoculate tumor cells into mice in the form of subcutaneous inoculation. Ability to extract immune cells and prepare them for analysis and/or further assays.
- Excellent oral and written communication skills and interpersonal skills
- Strong organization skills; ability to multi-task, prioritize and meet deadlines. Must demonstrate attention to detail and accuracy, time management skills, and follow-through.
- Ability to work effectively independently and collaboratively within a team. (Must be highly motivated, responsible, dependable and a self-starter).
- Proficiency in Microsoft Office (ex: Word, Excel, PowerPoint), knowledge of statistical software, array analysis software preferred.
- Must demonstrate willingness to learn new techniques, procedures, processes, and computer programs as needed.
- Experience with Immunohistochemical staining preferred.
- Experience with microarray data analysis preferred.
EDUCATION & EXPERIENCE:
Ph.D., M.D/Ph.D., or M.D. required in Microbiology and Immunology, Biotechnology, Molecular Biology, Oncology or other related field. Computer skills required; Power Point, Corel draw and other graphics software as preferred. Excellent skills in English (both verbal and written) required.