The Saligrama Lab at Washington University School of Medicine, Saint louis MO https://sites.wustl.edu/saligramalab/is seeking postdoctoral candidates to understand the adaptive immune responses in neurological disorders. The postdoctoral fellow will be conducting translational neuroscience research on individuals with Multiple sclerosis, Alzheimer Disease, and Parkinson Disease using cutting edge single cell technologies. In particular, we are interested in understanding the T cell receptor diversity, specificity, phenotype, and function at a single cell level using 10X genomics, CyToF, and proteomic approaches. This is an excellent opportunity for a life science graduate with immunology background to apply single-cell analysis approaches to tackle clinically relevant questions. These projects are funded by a recent grant from National Multiple Sclerosis Society. We are recruiting postdoctoral fellows who are eager to be part of a collaborative, interdisciplinary research environment that fosters diverse perspectives.
The Saligrama lab is part of The Bursky Center for Human Immunology and Immunotherapies Programs (https://burskycenter.wustl.edu) and the Hope Center for Neurological Disorders (https://hopecenter.wustl.edu). These centers have diverse array of cutting-edge technology core facilities.
- Gate D, Saligrama N, Leventhal O, Yang AC, Unger MC, Middeldorp J, Chen K, Lehallier B, Channappa D, De Los Santos MB, McBride A, Pluvinage J, Elahi F, Tam GK, Kim Y, Greicius M, Wagner AD, Aigner L, Galasko DR, Davis MM, Coray TW. (2020). Clonally expanded CD8 T cells patrol Alzheimers cerebrospinal fluid. Nature. doi:10.1038/s41586-019-1895-7
- Saligrama N, Zhao F, Sikora MJ, Serratelli WS, Fernandes RA, Louis MD, Ji X, Yao W, Idoyaga J, Mahajan VB, Chien Y-h, Hauser SL, Oksenberg JR, Garcia KC, and Davis MM. (2019). Opposing T cell responses in Experimental Autoimmune Encephalomyelitis. Nature. 572 (7770):481-487.
- Dulken BW, Buckley MT, Navarro Negredo P, Saligrama N, Cayrol R, Leeman DS, George BM, Boutet SC, Hebestreit K, Pluvinage JV, Wyss-Coray T, Weissman IL, Vogel H, Davis MM, and Brunet A. (2019). Single cell analysis of neurogenic niches during aging reveals age-dependent cell composition changes and interaction between T cells and neural stem cells. Nature. 24 (5):580-590.
- Mamedov MR, Scholzen A, Nair RV, Cumnock K, Kenkel JA, Oliveira JHM, Trujillo DL, Saligrama N, Zhang Y, Rubelt F, Schneider DS, Chien YH, Sauerwein RW, and Davis MM. (2018). A macrophage colony-stimulating factor producing gd T cell subset prevents malarial parasitemic recurrence. Immunity. 48 (2): 350-363.
- Satpathy AT*, Saligrama N*, Buenrostro JD*, Wei Y, Wu B, Rubin AJ, Granja JM, Li R, Lareau CA, Li R, Qi Y, Parker KR, Mumbach MR, Serratelli WS, Gennert DG, Schep AN, Corces MR, Khodadoust MS, Kim YH, Khavari PA, Greenleaf WJ, Davis MM, Chang HY. (2018). Transcript-indexed ATAC-seq for precision immune profiling. Nat. Medicine. 24(5), 580-590. (*Co-first authors, *contributed equally).
The position would suit a PhD graduate in molecular immunology. The appointee should have:
- Ph.D. in life science field (Immunology, Biochemistry/Biophysics, Cell Biology)
A comprehensive understanding of immunology, in particular T cell biology. High level proficiency in cell biology techniques such as FACS. Experience with relevant molecular biology techniques including PCR, DNA/RNA purification, cloning, and sequencing.
- Protein engineering background
Insect and mammalian expression of proteins and purification is preferred
- Computational skills (Big data, transcriptomics and genomics)
- Experience with CyToF, 10X genomics, and High dimensional data analysis
- Ability to work independently as well as collaboratively with a team
- Excellent publication record
This position is eligible for full-time benefits. Please click the following link to view a summary of benefits: https://hr.wustl.edu/benefits/.
If you are interested, please contact Dr. Saligrama by email (email@example.com) with
- A cover letter describing past research experience and future interests
- Complete contact information for three references
Information on being a postdoc at Washington University in St. Louis can be found at postdoc.wustl.edu/prospective-postdocs.
Washington University is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, age, sex, sexual orientation, gender identity or expression, national origin, genetic information, disability, or protected veteran status