Kuruman River Reserve

Postdocs Structural Biology of M. tuberculosis Membrane Proteins

Kuruman River Reserve

Leiden, Netherlands

The position
We have two post-doctoral positions available in the Lamers laboratory at the LUMC. The aim of the projects is to undertake the biochemical and structural characterization of two membrane proteins from the major pathogen Mycobacterium tuberculosis.

This work is part of a large European collaboration that aims to develop novel antibiotics against M. tuberculosis in the fight against ever increasing drug resistance in this bacterium. Your task will be to isolate the target membrane proteins and study these by structural (cryo-electron microscopy or protein crystallography) and biochemical methods and develop an assay suitable for high-throughput inhibitor screening.

Working at the LUMC
The LUMC is a center for medical innovation that aims to improve patient care through leading international research. So as to also provide our patients with a safe and friendly environment, we need doctors, medical care specialists, support staff and academic researchers.

The Lamers laboratory studies DNA replication and repair and their in role driving antibiotic resistance. The lab furthermore studies membrane efflux pumps that provide tolerance to all commonly used antibiotics. To study the different molecular processes, we use cryo-EM, protein crystallography, biochemistry and single molecule light microscopy. In previous studies, we have determined multiple structures of different protein-DNA complexes (Lamers, Cell 2006, Fernandez-Leiro, eLIFE 2015; Fernandez-Leiro Nat. Struct Mol Biol 2017). We have furthermore discovered a novel DNA proofreader in the replicative DNA polymerase from Mycobacterium tuberculosis (Rock, Nature Genetics 2015) and determined its structure (Mateos-Banos, Nat. Comm 2018). In addition, we have used single molecule light microscopy to monitor the sequential action of the different proteins during DNA replication (Zhao, eLIFE 2018). Our lab has the most advanced equipment for structural and biochemical research and are closely associated with the Netherland Center for Cryo-Electron Nanospectroscopy (NeCEN) that is equipped with two Titan Krios microscopes and is located 10 minutes from the laboratory.

Profile
You are a highly motivated candidate who:

  • has obtained a relevant PhD degree recently
  • has a strong background in structural biology and/or membrane proteins
  • preferably has experience with the topic M. tuberculosis

The offer
You will be employed on the basis of a 36-hour week. Appointment is for four years, for the duration of the project. The salary will depend on your qualifications and experience, with a maximum of € 4,361 gross per month based on a full time position (scale 10 of the Collective Labor Agreement for University Hospitals).

More information
A detailed description of this position can be found on www.werkenbijhetlumc.nl. If you have any questions or would like more information about this position, please contact Pauline Hoftijzer, management assistant, Department of Cell and Chemical Biology, telephone +31(0)71 529 70 64.

How to apply
If you are interested in this position, please complete the application form by the 2nd of June 2019 at the latest. Please include a motivation letter and a CV with two references with your application. You may also apply through the website www.werkenbijlumc.nl.

Link to application form: https://bit.ly/2VkFb33

Please apply via recruiter’s website.

Quote Reference: D.19.MV.CV.14

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