Post-doc position on the regulation mitotic transcription and chromatin architecture in S. cerevisiae
We announce a full-time postdoctoral position (36 months) in the group of Dr. Pierre Chymkowitch, Department of Microbiology, Oslo University Hospital, Oslo, Norway. The Dept. of Microbiology is a well-equipped internationally competitive research environment, and our group has an extensive collaboration network nationally and internationally. Finally, our research is interdisciplinary and has significant funding through the Research Council of Norway, Helse Sør Øst.
Project summary: The iconic X shape of condensed mitotic chromosomes has been described in the 19th century, but we still know little about the impact of mitotic condensation on chromatin functions like transcription. We have recently found that mitotic chromosome condensation is licensed by the centromere and spreads along chromosome arms in a chromosome-autonomous manner in S. cerevisiae (1). In parallel, other studies uncovered a mitotic-specific transcription program (2), but the molecular mechanisms establishing and maintaining mitotic transcription are yet unknown. This program seeks to uncover the mechanisms regulating mitotic transcription in the model organism S. cerevisiae. Based on other works and our preliminary data, we hypothesize that in addition to licensing chromatin condensation, centromeres control mitotic and interphase gene expression by impacting the transcription machinery. This project therefore seeks to identify new centromere functions and provide deeper insight into general mechanisms of gene expression and cell cycle regulation.
1) T. Kruitwagen, P. Chymkowitch, A. Denoth-Lippuner, J. Enserink, Y. Barral, Centromeres License the Mitotic Condensation of Yeast Chromosome Arms. Cell 175, 780-795 e715 (2018).
2) K. C. Palozola et al., Mitotic transcription and waves of gene reactivation during mitotic exit. Science 358, 119-122 (2017).
The candidate should ideally match the following profile:
- Owning a Ph.D. in Biology
- Extensive laboratory experience in molecular biology, cell biology and biochemistry
- Extensive experience with yeast genetics
- Experience in ChIP/ChIP-seq, RNA-seq, qPCR
- Experience with NGS Bioinformatics would be a plus
- Programming skills would be a plus
- Full professional proficiency in English
The application must include:
- Application letter – A brief account (one page maximum) of the applicant’s research interests and motivation for applying for the position
- CV (summarizing education, positions, administrative experience and other qualifying activity)
- Copies of educational certificates
- A complete list of publications
- Names and contact details of 2 references (name, relation to candidate, e-mail and telephone number)