Post-Doc position in CAR-T cell immunotherapy
Adoptive transfer of gene-modified T cells induces cancer regression in heavily pretreated patients. Treatment involves ex-vivo engineering of autologous lymphocytes, to express a chimeric antigen receptor (CAR) with specificity for a tumor-associated antigen. CARs are fusion proteins containing an antigen-recognition domain, derived from a single-chain antibody, and an intracellular signaling domain. A postdoctoral training opportunity is open in the Abate-Daga lab to perform research in the design, development, and validation of novel CARs, as well as mechanist studies of CAR-T cell immunobiology. Technologies to be implemented include: gene modification of human T cells using viral vectors, and non-viral platforms; genomic engineering using CRISPR/Cas9; system-level signaling analysis using phosphoproteomics and gene expression methods; among others. Preclinical validation of candidate constructs will be performed using murine models of adoptive cell transfer.
-Three years of experience in molecular biology techniques, cell culture, and flow cytometry.
-Proficiency in spoken and written English
-Background in systems biology.
-Hands-on experience in animal models.
-M.D. or Ph.D. in Immunology or relevant field.
Moffitt Cancer Center:
Located in Tampa, Moffitt is one of only 41 National Cancer Institute-designated Comprehensive Cancer Centers, a distinction that recognizes Moffitt’s excellence in research, its contributions to clinical trials, prevention and cancer control. Moffitt is the No. 1 cancer hospital in Florida and has been listed in U.S. News & World Report as one of “America’s Best Hospitals” for cancer since 1999. With more than 6,000 employees, Moffitt has an economic impact on the state of nearly $2 billion. For more information, visit MOFFITT.org, and follow the Moffitt momentum on Facebook, Twitter and YouTube.
Moffitt Cancer Center is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, or protected veteran or disabled status. We seek candidates whose skills, and personal and professional experience, have prepared them to contribute to our commitment to diversity and excellence.