Leiden University Medical Center (LUMC)

Postdoc in Transcription-coupled DNA Repair

Leiden University Medical Center (LUMC)

Leiden, Netherlands

In short
job vacancy number: D.19.MH.RD.05
closing date for application : December 1st 2019

The position
Are you fascinated by how DNA damage is repaired? We are looking for a postdoc to unravel the biochemical basis of transcription-coupled DNA repair in human cells.

The presence of DNA damage is a major complication during gene transcription. The transcription-coupled DNA repair (TCR) pathway ensures the efficient removal of transcription-blocking DNA damage from active genes, but the underlying mechanisms are not understood. Work in the Luijsterburg lab is focused on understanding these mechanisms. In the current project, funded by an ENW-klein grant from NWO, you will study the TCR pathway by employing biochemical, proteomics and genetics approaches to reveal how the TCR proteins work together and interact with each other to orchestrate efficient removal of transcription-blocking DNA damage. To this end, you will use CRISPR-Cas9-mediated genome editing, combined with protein cross-linking coupled with mass spectrometry (XL-MS) and proximity-dependent biotin identification (BioID).

The profile
You are an enthusiastic, flexible colleague who:

  • has obtained a PhD degree in molecular biology or a similar field
  • has an excellent publication record
  • preferably is experienced with research on DNA repair mechanisms
  • has solid skills in molecular and cell biology
  • is organized, accurate and possesses excellent communication skills in English
  • can work both independently as in a team

The offer
You will be employed on the basis of a 36-hour week. You will initially be appointed for one year with the possibility to extend this for two more years. The salary will depend on your qualifications and experience, with a maximum of € 4,481 gross per month based on a full time position (scale 10 of the Collective Labor Agreement for University Hospitals).

Working at LUMC
The LUMC is a center for medical innovation that aims to improve patient care through leading international research. So as to also provide our patients with a safe and friendly environment, we need doctors, medical care specialists, support staff and academic researchers.

The Department of Human Genetics is a broadly oriented research department, led by Professor Silvère van der Maarel, focusing on the genetic aspects of monogenic, multifactorial and acquired diseases, and the translation into clinical relevance. The department consists of 21 research units, embedded in four overarching research themes. You will be part of the research group of Dr. Martijn Luijsterburg and in one of the research laboratories that comprises the Genetic Instability and Cancer cluster. This cluster aims at elucidating the cellular response to different DNA-damaging agents and its relevance for human health, focusing on the mechanisms that underlie DNA repair and mutagenesis. The expertise and technical facilities of these DNA repair research groups are highly complementary, constituting a collaborative environment for cutting-edge research in the DNA repair field. Outstanding facilities for advanced live-cell imaging, genetic screening and interaction proteomics are available. The Luijsterburg group occupies a strong national and international position with collaborations with other academic partners across Europe and in the US and with strong links with patient organisations for DNA repair-deficiency syndromes.

More information
A detailed description of this position can be found on www.werkenbijhetlumc.nl. If you have any questions or would like more information about this position, please contact Dr. Martijn Luijsterburg, assistant professor, Human Genetics department, telephone +31 (0)71 526 96 12, email: s.m.luijsterburg@lumc.nl

References can be requested as part of the procedure.

How to apply
If you are interested in this position, please complete the application form by December 1st, 2019 at the latest. You can also apply through the website www.werkenbijhetlumc.nl.

Please apply via recruiter’s website.