Post Doctoral Fellow Position: Gene Editing and Genomic Evolution
Our laboratory at Northwestern University has an opening for a highly motivated post doctoral fellow to assist us with developing the most resistant human cells to ionizing radiation using state-of-the-art gene editing methodology. We have developed an information capability at the forefront of identifying gene alterations that affect the survival of mammalian cells to sparsely- (e.g. X-ray, rays) and densely-ionizing (heavy particle) irradiation. The overall impact of this work extends beyond its current focus of therapeutic resistance in cancer, including guiding strategies for the protection of mammals from radiation incidents, during space travel, and for the safe colonization of distant planets.
We seek to study the impact of different irradiation sources on mammalian genomes and counteract or minimize these effects by cis- and trans-gene interventions. We also seek to create conditions to accelerate genetic evolution driven by either the current templates of genes or de novo evolution; the latter is critical as sources of irradiation and their intensity may require an evolvable capability.
We offer a mentored research environment characterized by passion for science, the ability to acquire new and diverse skills, instruction in experimental and computational rigor, and where self-driven and enthusiastic trainees can thrive.
1. Abazeed, M.E. et al. Integrative radiogenomic profiling of squamous cell lung cancer. Cancer Res 73, 6289-98 (2013).
2. Kim, J.W. et al. Characterizing genomic alterations in cancer by complementary functional associations. Nat Biotechnol 34, 539-46 (2016).
3. Yard, B.D. et al. A genetic basis for the variation in the vulnerability of cancer to DNA damage. Nat Commun 7, 11428 (2016).
4. Yard, B.D. et al. Cellular and Genetic Determinants of the Sensitivity of Cancer to alpha-Particle Irradiation. Cancer Res (2019).
5. The radiogenomic atlas of human cancers. Manuscript in preparation.
• A trainee with an interest in a career in gene editing assay development, next-generation sequencing, functional genomics, and high-content screens.
• Self-motivated and goal-oriented biologist with experience in assay development and gene editing regardless of model system. Prior work with prokaryotes or lower eukaryotes is acceptable and will be viewed positively.
• Highly collaborative, resourceful and effective team-player who is enthusiastic to tackle challenging scientific and technical problems.
Desired Skill Sets
• Experience in assay development, gene editing, and sequencing
• Experience in primary cell culture
• Experience in barcode technology and next-generation sequencing
• Knowledge and hands-on experience with CRISPR/Cas9
• Working knowledge of applicable biology background for relevant topical areas
• Highly motivated scientist with particular attention to detail and the ability to independently plan, design and implement experiments
• Work cooperatively with multidisciplinary teams and present results at team meetings
Please send CV, statement of research interests, and three references to:
Mohamed Abazeed, MD, PhD
Associate Professor of Medicine
Scientific Director, Lung Cancer Program
Director, Center for Precision Radiotherapy
The Robert H. Lurie Comprehensive Cancer Center
Northwestern University is an Equal Opportunity, Affirmative Action Employer of all protected classes, including veterans and individuals with disabilities. Women, underrepresented racial and ethnic minorities, individuals with disabilities, and veterans are encouraged to apply. Hiring is contingent upon eligibility to work in the United States.