Lund University (LU)

Post doctor - diabetes research

Lund University (LU)

Lund, Sweden

Faculty of Medicine, Department of Experimental Medical Science

Lund University was founded in 1666 and is repeatedly ranked among the world’s top 100 universities.
The University has 40 000 students and 7 600 staff based in Lund, Helsingborg and Malmö. We are
united in our efforts to understand, explain and improve our world and the human condition.
The Faculty of Medicine is a part of Lund University, and is responsible for education and research
within medicine and healthcare. Our academic programs are closely linked with the healthcare system
and are firmly anchored in the faculty’s strong research tradition. Our research spans a broad field within
experimental preclinical research, near-patient clinical research and health sciences research. The Faculty
of Medicine, with its 1,800 employees and 2,700 students in Lund and Malmö, is a knowledge-intensive
meeting place for students, teachers and researchers from all over the world.

The Research Group

The project will be pursued in a lab with an excellent track record in diabetes research (headed by Nils
Wierup) https://www.ludc.lu.se/nils-wierup-pi. The Wierup lab is part of the Lund University Diabetes
Centre http://www.ludc.med.lu.se/. Lund University Diabetes Centre is one of the strongest centers for
diabetes research in the World.
The research Group is situated at Clinical Research Centre at the Scania University Hospital in Malmö in
southern Sweden.
Within the research group we aim to create a good work environment based on respect and consideration
in our relation to each other. We strive to create an environment that promotes development and is
stimulating to all employees. We persistently work on creating prerequisites for job satisfaction,
development and participation for all employees.
We are hereby adverstising for the position of a post doctoral fellow within diabetes research. The
position is full time for two years according to central agreement for temporary position as Post Doctor.
Desired start date is May 1st 2020 or according to agreement.

Project Description

Cell type-specific impact of T2D on the transcriptome will be assessed with 10X and SmartSeq2 single
cell RNA sequencing in islets from T2D patients and controls using established pipelines. Cell typespecific
Gene-regulatory networks (GRNs) will be computed using a novel algorithm. GRNs
representing key components of beta cell pathology have been identified (using the transcriptional
profiles of 5000 cells) and will now be subjected to functional validation. Gene expression profile will
be related to single cell functional properties, and potential subpopulations of alpha- and beta cells will
be assessed using Patch-seq. GRNs will be functionally validated using CROP-seq and whole genome
CRISPR-screens. Selected hub genes in the GRNs will be functionally validated using CRISPR-Cas9
gene targeting and state of the art techniques for assessment of beta cell function.

Work Duties

The project will be pursued at Lund University Diabetes Centre, one of the strongest centers for T2D research in the world. A wide group of collaborators will provide expertise, including bioinformatics and development of novel algorithms.
CROP-seq and CRISPR-screens will be performed in collaboration with SciLifeLab, Karolinska Institutet, Stockholm.
• The position will include a high degree of molecular biology wet lab work, patch clamp experiments as well as bioinformatic analysis and interpretation of data.
• As a Post Doctor you will be responsible for planning and running all experiments and finalizing scientific reports with a high degree of independence, but with close supervision by the group leaders and aid from our mentoring program.
• Other work duties within the research group can be performed as well.

Qualification requirements

A post doctor is an employee who has doctoral degree, or a foreign degree that is assessed to equal a doctoral degree. The applicant needs to have a PhD received maximum 3 years before the deadline of
this application. Special reasons may apply that change this time. For employment as a Post Doctor
following criteria will be applied:
• Good ability to develop and perfom research with good quality and educational skill.

A Post Doctor position should foremost consider scientific ability. Other assessments will be done according to the following requirements for the position:

• Applicants should have a Ph.D. in a relevant area.
• The project needs a highly motivated person with documented excellence in molecular- or
computational biology.
• Good communication skills in the English language (written and spoken) is a prerequisite.

Merits:

• A strong background in the diabetes field.
• Documented expertise in CRISPR-Cas9 technology, single cell RNAseq or patch clamp.

Instructions for the application:

CV, publication list with top 5 publications indicated, personal letter, reference letter from at least one internationally recognized senior scientist, diplomas of university degree and high school grades.

Lund University welcomes applicants with diverse backgrounds and experiences. We regard gender equality and diversity as a strength and an asset. We kindly decline all sales and marketing contacts.

Type of employment Temporary position longer than 6 months
Contract type Full time
First day of employment May 1st or according to agreement
Salary Monthly Salary
Number of positions 1
Working hours 100%
City Malmö
County Skåne län
Country Sweden

Contact Nils Wierup; Research group leader +4640391414
Sofia Sönnerstedt, HR-Coordinator +46462229876
Union representative OFR/ST:Fackförbundet ST:s kansli 046-222 93 62
SACO:Saco-s-rådet vid Lunds universitet 046-222 93 64
SEKO: Seko Civil 046-222 93 66

Please apply via recruiter’s website.

Quote Reference: PA2020/404