Institute of Laboratory Medicine, Clinical Chemistry and Molecular diagnostics
20 hours / week
limited contract (until 31.12.2020, with possibility of extension)
Salary scale of the University hospital Leipzig (UKL)
The earliest possible joining date is 01.12.2019
A PhD student position is available in the research group of Prof. Berend Isermann at the Institute of Laboratory Medicine at Leipzig University. The project aims at studying the role of coagulation proteases dependent signal transduction on the interaction between epithelial cells, endothelial cells and the immune system in regard to barrier function. The main focus is to study cell-cell-interaction at defined barriers using in-vitro systems like microfluidics and iPS derived organoid culture. Please also have a look at our publications: Ranjan, Isermann, Nature Commun 2017; Nazir, Isermann, Blood 2017; Kohli, Isermann, Blood 2016; Madhusudhan, Isermann, Nature Commun 2015; Shahzad, Isermann; Kidney Int 2015.
The project involves:
- Applying various cell culture methods to co-culture different cell types and to analyze maladaptative cellular communication
- Investigation of induced pluripotent stem cell (iPS)-derived organoids
- Microfluidic cell cultured models to mimic intercellular interaction and to analyze barrier associated processes in real time and under flow conditions.
- Investigation of epigenetic modulation that regulates metabolic programming (in vitro and in vivo)
- Applying various methods of ex vivo analysis, including co-immunoprecipitation, ChIP-on-chip assay, metabolomics and single-cell sequencing
- Microscopic analysis techniques such as live cell imaging, immunohistochemistry, immunofluorescence and electron microscopy
- Successful completion of master’s degree in life sciences (biology, biochemistry, biotechnology)
- Relevant research experience (preferably international) and good English communication skills
- Comprehensive detailed knowledge in molecular biology, cell culture, mRNA, protein and DNA analysis epigenetics and cloning techniques
- Comprehensive detailed knowledge in cell culture, cell migration, cell proliferation, co-culture models and cell death are required
- In depth knowledge and experience in live-cell-imaging and microscopy techniques is desirable.
- Applicants with experience in stem cell research and/or flow chamber based microfluidics experiments are preferred
As a public employer in the heart of Leipzig, we stand for a corporate culture in which cooperation for the best care of our patients is paramount. As a maximum provider with 27 clinics and about 4,000 employees, we provide groundbreaking technical specialization as well as state-of-the-art structural and technical infrastructure.
Please send your applications not later than 30.11.2019 to firstname.lastname@example.org.
Your application should include:
1) Full curriculum vitae, publication list, information on years of experience in research and laboratory work including the methods you already know and you are experienced;
2) Names and contact information (email and telephone numbers) of 3 references;
3) A summary of research interests and long term plans;
Only shortlisted applicants will be notified.
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For information on the application process, please contact Dr. Ronald Biemann at 0049 341 / 97-22455 or email@example.com
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