PhD position with a focus on characterization of protein-protein interactions surfaces using cross-linking mass spectrometry, computational modelling and experimental mutagenesis

Jülich Research Centre (FZJ)

Jülich, Germany

Work group:

ICS-4 – Zelluläre Biophysik

Area of research:

PHD Thesis

Job description:

Your Job:

In excitable cells, voltage-gated calcium channels open upon depolarization and couple electrical activity to neurotransmission, muscle contraction, endocrine gland secretion and gene expression. The actin-based cellular cytoskeleton provide the scaffold for the cellular organization and the tracks for the intracellular trafficking of the channel toward and from the plasma membrane. The association between voltage-gated calcium channel and actin filaments regulates synaptic transmission. This project aims to characterize the calcium channel/actin interaction in health and disease states at the molecular level using a multi-methodology platform.Your tasks in detail:

Performing crosslinking, mass spectrometry and information-guided computational modeling of the protein complex Validation of the generated model using mutagenesis and cell-based assays

Your Profile:

Master degree in Biology, or related natural sciences disciplines (Biochemistry, Biotechnology, Biomedicine, Pharmacy or Molecular Medicine) Strong interest in structure-function relationship of membrane proteins Methodological competence in standard biochemical and molecular biology techniques Experience in mass-spectrometry and bioinformatics is of advantage High degree of commitment, and ability to work in a team in different environments Good communication and written skills in English

Our Offer:

Outstanding technical infrastructure – ideal conditions for successfully completing the suggested project for a doctoral degree Opportunity to get expertise in state-of art techniques and strategies in the field of innovative methods for analyzing protein-interaction networks and probing disease mechanisms caused by genetic mutations Embedding in a multidisciplinary environment where you will be in continuous contact with scientists of three different institutes within Forschungszentrum Jülich Continuous scientific mentoring and discussions by the three scientists directing this project with expertise in cross-linking, cell-based assays, mass spectrometry analysis and computational modeling A contract for the duration of 3 years Further development of your personal strengths, e.g. via a comprehensive further training programme The doctoral degree will be conferred by the Heinrich Heine University, Düsseldorf Pay in line with 50 % of pay group 13 of the Collective Agreement for the Public Service (TVöD-Bund) Information on employment as a PhD student at Forschungszentrum Jülich can be found here (

Forschungszentrum Jülich aims to employ more women in this area and therefore particularly welcomes applications from women. We also welcome applications from disabled persons.

Please apply via recruiter’s website.

Quote Reference: 2019D-150