Higher Scientific Officer – in vivo (maternity cover)
Division Breast Cancer Research
Team Gene Function
Vacancy Type Full time
Type of Contract Fixed Term
Length of Contract Up to 1 year
Hours per Week 35
Salary Range £33,285 – £41,437
The Institute of Cancer Research, London, is one of the world’s most influential cancer research institutes, with an outstanding record of achievement dating back more than 100 years. We provided the first convincing evidence that DNA damage is the basic cause of cancer, laying the foundation for the now universally accepted idea that cancer is a genetic disease. Today, The Institute of Cancer Research (ICR) leads the world at isolating cancer-related genes and discovering new targeted drugs for personalised cancer treatment.
Under the leadership of our Chief Executive, Professor Paul Workman FRS, the ICR is ranked as the UK’s leading academic research centre. Together with our partner The Royal Marsden, we are rated in the top four cancer centres globally.
The ICR is committed to attracting, developing and retaining the best minds in the world to join us in our mission – to make the discoveries that defeat cancer.
We are seeking a highly motivated HSO to provide technical support to the in vivo research team within the Gene Function Laboratory to study synthetic lethal effects that operate in cancer, with the express aim of developing better ways to treat the disease.
The post-holder will work closely with the newly formed patient-derived models (PDM) team within the Breast Cancer Now Research Centre (BCNRC). This team provides expertise and supports experiment design for the Centre, to enable establishment and use of patient-derived xenograft (PDX) models for translational studies with the Gene Function Laboratory. As part of the team, he/she will work under the supervision of Dr Rebecca Marlow (Liaison Staff Scientist), under the direction of Prof. Tutt and Prof. Lord. Dr Marlow also supervises the 3D Facility team under the direction of Dr Eleanor Knight and this HSO post will provide material for the derivation and production of patient-derived organoid (PDO) cultures. The 3D Facility carries out complex 3D cell culture experiments with group members within the Centre.
The post-holder will provide support to the in vivo work within the research context of developing breast cancer models, as well as other transgenic breast and ovarian cancer models that investigate factors that promote disease progression, and metastasis of interest to the Tutt and Lord laboratories. The post-holder may also provide support for the research projects within Gene Function Laboratory that are investigating other topics including gain of resistance to therapeutic treatment and will be involved in the development of new techniques of interest to the research programme in collaboration with the Lord and Tutt groups, such as the mammary intraductal model (Sflomos et al 2016 PMID: 26947176).
The successful candidate will be responsible for maintaining and characterising genetically engineered murine models of breast and ovarian cancer (Molyneux et al 2015 PMID: 20804975; Chandler et al 2015 PMID:25625625), and organizing, performing, and reporting on in vivo studies. This will include, but is not limited to, animal handling and health monitoring, drug administration, xenograft/allograft/orthotopic modelling, in vivo model imaging (including IVIS) and accurate database keeping and maintenance. Experience of working with animals is essential, together with ability to perform standard laboratory in vitro techniques.
You are expected to participate in the day-to-day running of the in vivo team, including ordering supplies, maintaining inventories and assistance in the generation and managing of cell line and viably frozen tissue stocks for xenograft studies and patient derived xenotransplantation breast cancer models. Additionally, you will provide scientific and technical support for on-going laboratory work to assist in the validation of PDX models e.g. PCR, preparation of samples for histology. This will include generating and analysing data from transgenic models as well as patient derived xenograft studies. The post-holder will demonstrate good interpersonal skills and collaborative working practices. Excellent organizational and communication skills are essential.
Applicants must have a degree in Biochemistry, Molecular Biology or Cell Biology and in-depth technical laboratory experience in data management, standard biochemical, molecular biology and cell biology laboratory techniques. Experience using in vivo models is essential and use of of breast and/or ovarian cancer models, xenografting and transplantation techniques are highly desirable. Knowledge of cancer biology, 3D cell culture and assay methodologies, and familiarity with contemporary approaches to generation of genetic modifications, such as use of CRISPR, would be advantageous.
The post will be primarily based in the ICR, Chelsea. On regular occasions, the appointed candidate will also interact with Dr Marlow’s in vivo team within Professor Tutt’s laboratory at King’s College London (KCL). The post-holder will closely interact with other researchers within the Tutt and Lord groups.
This appointment is to provide maternity cover on a fixed term contract for up to one year, on the Higher Scientific Officer scale. The full salary scale is within the range of £33,285 – £41,437 per annum inclusive, based upon relevance of skills and experience. Please be aware that the appointment could terminate earlier, dependant on the duration of the substantive post holder’s absence.
Informal enquiries can be made via email to Dr Rebecca Marlow (firstname.lastname@example.org). Please note – this address is for enquiries only and you should not send your application to this email address.
We consider all applications on merit and have a strong commitment to enhancing the diversity of our staff.