Electron Microscopy Research Associate

Yale University, United States

New Haven, CT, United States

Yale University offers exciting opportunities for achievement and growth in New Haven, Connecticut. Conveniently located between Boston and New York, New Haven is the creative capital of Connecticut with cultural resources that include three major museums, a critically-acclaimed repertory theater, state-of-the-art concert hall, and world-renowned schools of Architecture, Art, Drama, and Music.


General Purpose: 

Under the direction of the Principal Investigator and working in collaboration with laboratory personnel, this position will be in charge of the design, collection, optimization and interpretation of electron microscopy research in the Colón-Ramos lab as it pertains to the ultrastructure of C. elegans nervous system. Work includes preparation of scientific reports and papers for research proposals and published records. The position will prepare and process C. elegans worms for electron microscopy to better understand the function and structure of the C. elegans nervous system. Specific duties include but are not limited to:


Review literature and data generated in the lab in order to recommend the design of new experiments and protocols to address the research questions laid out by the PI. Recommend new research questions based on findings.


Analyze, develop, and interpret all the data collected through EM techniques.


Work with lab members and extramural collaborators to coordinate projects and approaches.


Prepare electron microscopy data for presentations, including published scientific reports and seminars.


Sample preparation techniques, including:

 Fix C. elegans larvae and embryos by high pressure freeze and freeze substitution (HPF/FS).

 Embed, trim, and orient fixed samples. Cut and stain serial sections at specific ROI.

 Prepare samples for immunolabeling and perform immunogold staining.


Process electron micrographs, including montaging, alignment, segmentation, and 3D rendering.


Perform advanced microscopy methods such as electron tomography, Focus-Ion-Beam SEM (FIB-SEM) 3D volume imaging as needed by projects.


Required Education and Experience: 

Master’s Degree in a scientific discipline and one year experience or an equivalent combination of education and experience.


Qualifications:

  • Ability to coordinate laboratory projects, establish and maintain effective working relationships with supervisors, collaborators and other employees.
  • Well-developed understanding of procedures and techniques used in Electron Microscopy with the ability to adapt to new procedures concerning work in electron microscopy of C elegans.
  • Ability to perform tasks requiring a high degree of attention to detail.
  • Well-developed verbal communication skills.
  • Preferred Education and Experience: Master’s Degree in biology or Neuroscience and one year experience. At least 3 years of experience in biological electron microscopy. Desired expertise in immuno electron microscopy, FIB-SEM and electron tomography (room temperature) for biological sciences. Knowledge of Cell Biology and Neuroscience and experience working with teams.


Application: For more information and immediate consideration, please apply online at http://bit.ly/YaleCareers-59171BR. Please be sure to reference this website when applying for this position.


We invite you to discover the excitement, diversity, rewards and excellence of a career at Yale University. One of the country’s great workplaces, Yale University offers exciting opportunities for meaningful accomplishment and true growth. Our benefits package is among the best anywhere, with a wide variety of insurance choices, liberal paid time off, fantastic family and educational benefits, a variety of retirement benefits, extensive recreational facilities, and much more.

Yale University considers applicants for employment without regard to, and does not discriminate on the basis of an individual’s sex, race, color, religion, age, disability, status as a veteran, or national or ethnic origin; nor does Yale discriminate on the basis of sexual orientation or gender identity or expression.



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Position

Electron Microscopy Research Associate