Karolinska Institutet (KI)

Doctoral (PhD) student position in Immunology and Gene Therapy

Karolinska Institutet (KI)

Huddinge, Sweden

A doctoral student is admitted to third-cycle/doctoral education. A doctoral education consists of supervised research, combined with courses and other educational activities. An individual study plan is established for each doctoral student. For a doctoral degree, the equivalent of four years of full-time doctoral education is required.

Division
The Cell and Gene Therapy Group at the Department of Medicine Center for Hematology and Regenerative Medicine is announcing a doctoral student position at Karolinska Institutet within the research fields of Hematology, Cancer Immunotherapy and Gene Therapy.

Research group  
The Cell and Gene Therapy Group has a particular interest in exploring natural killer (NK) cells in patients with multiple myeloma (MM). MM is a monoclonal plasma cell malignancy representing 10% of all hematological cancers. NK cells are a vital part of the innate immune system, capable of tumor cell killing. Recently, a population of NKG2C+ NK cells has been identified in about 50% of CMV+ individuals, termed adaptive natural killer cells. These cells are long-lived and provide a superior capacity to proliferate and to perform antibody-dependent cellular cytotoxicity (ADCC). The group has internationally-recognized leadership in clinical grade, current good manufacturing practice (cGMP)-compatible manufacturing of cellular and gene therapy applications. Furthermore, we have been involved in a large number of clinical trials, including the first Swedish clinical trials assessing the safety of both patient and donor derived ex vivo expanded NK cells in patients with various malignancies.

The Cell and Gene Therapy Group is a multidisciplinary research group with 15 members including 2 PhD students. The primary project supervisors will be Evren Alici, Arnika Wagner, Stephan Meinke and Hans-Gustaf Ljunggren. The PhD student will belong to the Center for Hematology and Regenerative Medicine (HERM), a translational research center encompassing research groups involved in hematology, hematopoiesis, immunology, and cell therapy.

Research environment
The student will work at HERM with labs and platforms situated at both NEO and ANA Futura. HERM is a dynamic research center focused on hematology and cancer immunology. The primary labs, located at ANA Futura, provide a variety of resources and equipment needed for executing state-of-the-art research, including access to a flow cytometry facility, molecular biology resources and equipment, culture systems for a variety of cell lines and primary healthy donor and patient material. The research group has weekly progress meetings where students provide updates on their progress and receive feedback from the team.

In addition, the group is a part of a VINNOVA funded competence center which is a program focused on next-generation NK cell therapies. The prospective student will be working in the multidisciplinary translational environment that the competence center creates.

The doctoral student project and the duties of the doctoral student 
We are recruiting a student for a doctoral education. A doctoral education (PhD) consists of supervised research, combined with courses and other educational activities. An individual study plan is established for each doctoral student. For a doctoral degree, the equivalent of four years of full-time doctoral education is required. For more information regarding PhD education at Karolinska Institutet, see: http://www.ki.se/doctoral.

The project consists of three independent yet interconnected aims, which involve a mix of lower to higher risk-taking. Projects can, in essence, be performed in parallel. In the first and the second project, the aim will be to assess phenotype and functions of adaptive NK cells in MM patients undergoing various treatment options, including monoclonal antibody therapy. The overall goal is to identify optimal combinations of NK cells together with other treatment modalities and to increase the efficacy of NK cell products for therapy of MM. In a third project, NK cells will be genetically engineered in order to regulate their cytotoxic and cytokine effector functions.

These projects will include immunology, cancer and cell biology, molecular biology, several in vitro methods and potentially in vivo experiments in mice. We are planning to perform genome-wide analyses such as single cell RNA sequencing and CRISPR screens for the identification of relevant genes.

The successful applicant will acquire basic as well as specialized knowledge and understanding in immunology and tumor biology and how to address research questions within these topics. The PhD student will learn to apply, master, and develop research methodologies in immunology and tumor biology, in which the group is specialized. Examples of skills that will be explored include advanced flow cytometry and imaging techniques, retro/lentiviral work, state-of-the-art molecular biology experimentation, and work with human cells and animal models for tumor immunology.

Skills and personal qualities  
We are seeking a highly motivated and enthusiastic candidate to work on projects focused on NK cells and cancer research. You should be collaborative, scientifically adventurous, curiosity-driven and like team-work in an international environment. In addition, you should be prepared to assume responsibility to accomplish your goals in the project(s) and fulfil the requirements of the doctoral training program.

The PhD student is expected to take a strong lead in project management, data generation, as well as analyses and interpretation. The duties also include working in a team and to adhere to high scientific standards and responsibilities.

The applicants are expected to be talented and enthusiastic researchers with a strong research drive. Prior experience with cell culture, molecular biology and flow cytometry is desirable. To be able to elaborate in scientific hypotheses, experimental design, experimentation, and presentation/publication of results is expected. Authorship on research articles is highly meriting, as is previous training in research laboratories. Candidates must be able to work within a heterogeneous and multicultural team environment but retain independence to explore their own hypotheses and results and drive their own projects. Personal suitability will be a selection criterion.

Eligibility requirements for doctoral education
In order to participate in the selection for a doctoral position, you must meet the following general (A) and specific (B) eligibility requirements at latest by the application deadline.

It is your responsibility to certify eligibility by following the instructions on the web page Entry requirements (eligibility) for doctoral education.

A) General eligibility requirement

You meet the general eligibility requirement for doctoral/third-cycle/PhD education if you:

  1. have been awarded a second-cycle/advanced/master qualification (i.e. master degree) or
  2. have satisfied the requirements for courses comprising at least 240 credits of which at least 60 credits were awarded in the advanced/second-cycle/master level, or
  3. have acquired substantially equivalent knowledge in some other way in Sweden or abroad.

Follow the instructions on the web page Entry requirements (eligibility) for doctoral education.

If you claim equivalent knowledge, follow the instructions on the web page Assessing equivalent knowledge for general eligibility for doctoral education.

B) Specific eligibility requirement

You meet the specific eligibility requirement for doctoral/third-cycle/PhD education if you:

  • Show proficiency in English equivalent to the course English B/English 6 at Swedish upper secondary school.

Follow the instructions on the web page English language requirements for doctoral education.

Verification of your documents Karolinska Institutet checks the authenticity of your documents. Karolinska Institutet reserves the right to revoke admission if supporting documents are discovered to be fraudulent. Submission of false documents is a violation of Swedish law and is considered grounds for legal action.

Terms and conditions
The doctoral student will be employed on a doctoral studentship maximum 4 years full-time.

Application process
Submit your application and supporting documents through the Varbi recruitment system. Use the button in the top right corner and follow the instructions.

Your application should contain the following documents:

  • A personal letter and a curriculum vitae (Swedish or English)
  • Degree projects and previous publications, if any (Swedish or English)
  • Any other documentation showing the desirable skills and personal qualities described above (Swedish or English)
  • Documents certifying your general eligibility (see A above)
  • Documents certifying your specific eligibility (see B above)

For more information regarding doctoral (PhD) education, see: http://www.ki.se/doctoral.

Selection
A selection will be made among eligible applicants on the basis of the ability to benefit from doctoral education. The qualifications of the applicants will be evaluated on an overall basis.

KI uses the following bases of assessment:

  • Documented subject knowledge of relevance to the area of research
  • Analytical skill
  • Other documented knowledge or experience that may be relevant to doctoral studies in the subject.

 All applicants will be informed when the recruitment is completed.

Please apply via recruiter’s website.

Quote Reference: 297656

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