The Synthetic Biology Tools for Yeast section at the Novo Nordisk Foundation Center for Biosustainability (DTU Biosustain), at the Technical University of Denmark (DTU), is seeking a skilled analytical chemist for compound identification.
DTU Biosustain is focused on developing the next generation of cell factories deploying advanced metabolic engineering techniques, and the development of bioprocesses for a sustainable production of chemicals and high value compounds.
Focus and scope
The Synthetic Biology Tools for Yeast group is focused on refactoring of plant biosynthetic pathways for human therapeutics manufacturing in microbial cells. The competencies in our laboratory, led by Prof. Jay D. Keasling and Dr. Jie Zhang, focus on the use synthetic biology to engineer microbes for production of therapeutics otherwise difficult to source. To strengthen our team’s translational R&D activities, and further advance our visions for delivery of new supply chains for bioactive natural products we seek an analytical chemist with a focus on:
- Analysis and quantification of known compounds in biological samples (targeted compound analysis/metabolite profiling)
- Analysis and relative quantification of unknown compounds in biological samples (metabolomic analysis)
- Analysis and identification of unknown compounds in biological samples (compound ID)
- Analytical method development for quantitative and quanlitative analyses in biological samples
The candidate will be responsible for developing and executing state-of-the-art compound identification with main focus on extraction, isolation and MS-based characterization of bioactive natural products, and support HR-MS analysis for mitigating metabolic engineering bottlenecks. The candidate will be affiliated with the analytical core team at DTU CfB and is expected to closely collaborate with a team of 5-10 researchers with expertise within strain engineering, biochemistry, pathway discovery, and synthetic biology. The candidate will be responsible for the daily operation of the analytical equipment. Key responsibilities will include:
- Extraction, isolation and identification of compounds from microbial sources
- GC- & LC-MS/MS method development and validation
- GC- & LC-MS/MS data analysis
- Writing and maintaining SOPs
- Setting up protocols and training other personnel to the highest standards
The ideal candidate will have:
- Significant experience working in a relevant environment (e.g. compound ID, natural products), with a solid analytical chemistry background
- Proven experience in chromatography method development, instrument maintenance, mass spectrometry & high throughput analysis
- Expertise/experience in de novo compound identification
- Experience in interpretation of complex mass spectra
- Proven ability to work creatively in a team environment
- Proactive attitude and a translational research drive
- Strong oral and written skills in English
- Experience working in biological systems
- Flexibility, enthusiasm, responsibility, team spirit and excellent collaboration skills.
- Competence and experience in natural product- and analytical chemistry techniques for chemical profiling, dereplication, isolation and identification, especially through HPLC, LC-MS, and other techniques
- Experience in software for natural product and mass data processing
Application and contact
Please submit your online application no later than 5 January 2020.
To view the full announcement and to apply: www.career.dtu.dk