Wellcome Trust Sanger Institute

Advanced Research Assistant - Cellular Generation and Phenotyping

Wellcome Sanger Institute

Cambridge, United Kingdom

An opportunity has arisen for two talented cell biology specialists to support the Cellular Generation and Phenotyping team (CGaP) at the Sanger Institute. One role is permanent, and the other is a three-year fixed term post.

At Sanger, we appreciate the importance of achieving work-life balance and support this with a number of family and carer-friendly policies. We will equally consider applications from candidates working either full or part time hours (minimum 25 hours over 4 days a week). Please specify your preferred working hours and pattern in your application.

Please see the following job overview for each role:

Role 1:
You will be working as a part of a team on induced pluripotent stem cell (iPSC) differentiation into neuronal lineages using both wild type and knock out iPSCs.

As an Advanced Research Assistant, you will play a fundamental role in expansion, banking and differentiation of iPSCs into neural stem cells and terminally differentiated neurons. Working in partnership with faculty teams you will enable the project to run at scale. The role requires you to aid in tracking the progress of the project to ensure a high quality of work and see the project through to completion.

You will be part of an experienced team of enthusiastic, highly motivated Research Assistants, under the management of a Senior Scientific Manager. Your role will include the culture and manipulation of iPSCs in the lab; co-ordinating the transfer of samples to and from the faculty team; troubleshooting issues; identifying areas for process improvement; and training more junior staff. In addition to the lab work, we expect our Advanced Research Assistants to have strong time management and organisational skills and be excellent communicators.

Role 2:
You will join a multi-disciplinary team performing genome-wide CRISPR-Cas9 knockout screens on cancer and iPSC lines to investigate gene essentiality on an individual patient level.

As an Advanced Research Assistant within these projects, you will perform large-scale culture of cancer and iPSC lines, transduction of these cell lines with lentivirus and quality control analysis using a range of assays such as flow cytometry and viability assays. This will require working as a leading member of a team to manage the process within the lab, train junior staff and drive development of the cell biology. You will support management in reporting on project progress and ensuring good communication with the faculty group for sample transfer and troubleshooting.

Department ethos
As a department we work together to deliver large-scale projects successfully, building a culture of respect, cohesiveness and continuous process improvements. We will provide you with all the appropriate training for you to fulfil your role and offer you a career pathway so you can continue to progress and learn new skills. We encourage all staff to take part in the learning and development opportunities available at the Institute, as well as department and institute wide initiatives.

More about CGaP and the department’s portfolio of projects:
We are a core research group, responsible for providing cell biology resource and expertise to the wider institute. We do this by running multiple, diverse, high-throughput projects for various faculty teams from across the Sanger Institute. Our current portfolio of projects include iPSC derivation, differentiation of iPSCs toward myeloid and neuronal lineages, genome wide CRISPR screens of iPSC and cancer cells, organoid derivation, the Human Cell Atlas Initiative. These are the projects of today but the scope for what CGaP does is endless. Our role is to support the institute in its aims and for that we continually strive to remain at the forefront of cutting edge research. Namely translating academic research into large-scale projects, which push the boundaries of our understanding, allowing the Sanger to remain a world leader in genome research.

This is an excellent opportunity for a motivated and adaptable individual to join the team in delivering high quality science.

Essential Skills

• Degree in Biological Sciences or an equivalent qualification, with 2-3 years relevant laboratory experience
• Extensive experience culturing mammalian cell lines
• Experience of running cell based assays or antibody staining techniques
• Ability to test and implement new approaches and techniques into new SOPs

Skills & Attributes
• Ability to troubleshoot and process improve
• High attention to detail and excellent record keeping
• Excellent organisational and communication skills
• Proven ability to be able to prioritise, organise and schedule their own work
• Proven experience of working within a team

Ideal Skills

• Experience of neuronal iPSC differentiation (Role 1)
• Experience of use of CRISPR-Cas9 in cell lines (Role 2)

Skills & Attributes
• Experience collaborating with others and effectively working in teams
• Experience of training others
• Proven track record in generating and analysing high quality data

Other information
Application Process

Please apply online and include a covering letter with your application describing how you meet the essential criteria for the role, your preferred working hours and pattern and any role preference.
Closing date: 06 April 2020

About Us
The Wellcome Sanger Institute is a world leading genomics research centre. We undertake large-scale research that forms the foundations of knowledge in biology and medicine. Our findings are used to improve health and to understand life on Earth. Find out more at www.sanger.ac.uk or follow us on Twitter, Facebook, LinkedIn, Youtube and on our Blog.
Our campus
Set across 130 acres, just outside of Cambridge, The Sanger Institute is located on the stunning Wellcome Genome Campus. Home to some of the world’s foremost institutes and organisations in genomics and computational biology, committed to delivering life-changing science with the reach, scale, and creativity to solve some of humanity’s greatest challenges.

Our benefits:
There’s an attractive benefits package on offer at the Genome Wellcome Campus. We appreciate the importance of achieving work-life balance and support this with a number of family and carer-friendly policies. Plus a flexible working policy for those who may wish to amend their working pattern or arrangement.
As well as the usual benefits you would expect, we go much further:
• 25 days annual leave (extra 1 day to a maximum of 30 days for every year you work)
• Auto-enrolment into a generous Group Defined Contribution Pension Scheme, with enhanced company contribution (for more information, see our Pensions page)
• Up to 2 days annual paid volunteering leave
• Up to 10 days paid Emergency Carers Leave per year
• Family friendly environment including options for flexible and part-time working, an on-site Workplace Nursery salary Sacrifice Schemes for pre-school children and Summer holiday club
• Life Assurance Cover and a Group Income Protection Scheme (if on a contract of 12 months or more)
• Enhanced maternity leave and parental leave
• Access to substantial number of courses and training events onsite
• Private Healthcare Scheme (after 6 months service)
• Eyecare and Dental payment plans
• Concessions and discounts from our corporate perks site

Being part of the Genome Wellcome Campus you will be part of the beautiful working environment with an impressive range of benefits, services and facilities also including:
• Free bus service to and from Campus, covering various routes around Cambridge, Saffron Walden and surrounding villages
• A car-share initiative
• Free parking
• A number of on-site venues where you can meet, eat and socialise with colleagues
• A thriving Sports and Social Club which provides members with subsidised access to a gym, tennis courts, sports hall, fitness classes and a vibrant social calendar of events
Find out more about our Genome Wellcome Campus.

We hold an Athena SWAN Bronze Award and will consider all individuals without discrimination and are committed to creating an inclusive environment for all employees, where everyone can thrive.

Please apply via recruiter’s website.