EMBO Practical Course: Quantitative proteomics: strategies and tools to probe biology

EMBL

11 June 2023

EMBL Advanced Training Centre

Registration Deadline

19 March 2023
This course will provide both theoretical and practical training in the use of quantitative proteomics approaches. Participants will learn the principles of current methodologies including label-free, SILAC and TMT as well as targeted proteomics, and they will be trained in the use of bioinformatic tools for data analysis.

Audience
This course is aimed at researchers who know the basics of proteomics and who want to get a deeper understanding of quantitative methodologies, and at those who are familiar with wet-lab proteomic experimentation but want to gain experience in bioinformatic data interpretation. To enter the course a basic understanding of mass spectrometry-based proteomics is beneficial.

Modules/resources
The principles of proteome quantification by stable isotope labeling (SILAC and TMT), label-free, and targeted proteomics
Introduction how various types of mass spectrometers can be used for proteome quantification
Computer training sessions using software tools for the analysis of various quantitative proteomic data types
Lectures demonstrating the application of quantitative proteomics in biology, clinical research and drug discovery
Learning outcomes
Understand the principles and use cases of quantitative proteomic methods
Ability to design quantitative proteomic experiments, including selection of the most appropriate hardware and software
Ability to analyse quantitative proteomic data by various software tools