This course is intended for PhD students and Postdoctoral researchers who are interested in learning how to work with extracellular vesicles and know more about their biomedical applications.
Extracellular vesicles (continuous and discontinuous density gradient ultracentrifugation, ultrafiltration techniques, bead-capture and precipitation techniques, size-exclusion chromatography (SEC)
Extracellular vesicles characterisation (Immunoblot and electron microscopy, fluorescent labeling, quantitative and qualitative analysis by flow cytometry, nanoparticle tracking analysis, in vivo imaging, proteomic and transcriptomic analysis of EV)
Extracellular vesicles composition (lipidomics, proteomics, transcriptomics)
Extracellular vesicles and function (molecular players for extracellular vesicle biogenesis, extracellular vesicle targeting to cells, extracellular vesicles as diagnostics and/or therapeutics in pathophysiology)
In this course we intend to make PhD students and post-doctoral researchers familiar with good-practice-guidelines and methods for purification and identification of extracellular vesicles with practical sessions included.