Volume 560 Issue 7718, 16 August 2018

Volume 560 Issue 7718

Rolling clones

The cover image shows a number of marbles rolling down a slope. Much like the cell lines they represent, these marbles all look superficially similar, but on closer examination those farther down the slope are showing changes in their internal patterning, which reflect the results found in this week’s issue by Todd Golub and his colleagues. Looking at cancer cell lines, the researchers show that far from being clonal, the cell line ‘strains’ display differences in genomic alterations, gene-expression profiles, proliferation rates and drug response, all of which arise as a result of genetic and transcriptional evolution. Their findings have implications for cancer research using such cell lines and the team has created a publicly available online tool called Cell STRAINER to help assess cell-line divergence.

Cover image: Iris Fung with contributions from Andrew Tubelli, Bang Wong and Uri Ben-David

This Week

News in Focus

Comment

Careers

Futures

  • Futures |

    Time to publish.

    • J. W. Armstrong

Research

    News & Views

  • News & Views |

    Catalysts working in pairs can promote more-effective reactions than can the same catalysts used sequentially. The coupling of an enzyme with a light-activated catalyst offers great potential for organic synthesis.

    • Nicholas J. Turner
  • News & Views |

    Humans perceive colours in categories such as red, even though we can discern red hues including ruby and crimson. It emerges that birds also categorize colours and this affects their colour-discrimination ability.

    • Almut Kelber
  • News & Views |

    RNA sequencing of single cells in the mammalian trachea reveals a previously unknown airway cell that expresses genes involved in fluid and solute balance, and that might play a part in cystic fibrosis.

    • Kyle J. Travaglini
    •  & Mark A. Krasnow
  • News & Views |

    Neutrinos are tiny, ghost-like particles that habitually change identity. A measurement of the rate of change in high-energy neutrinos racing through Earth provides a record-breaking test of Einstein’s special theory of relativity.

    • Matthew Mewes
  • News & Views |

    Genome-editing approaches have been used to fuse 16 yeast chromosomes to produce yeast strains with only 1 or 2 chromosomes. Surprisingly, this fusion has little effect on cell fitness.

    • Gianni Liti
  • Articles

  • Article |

    Single-cell RNA sequencing analysis identifies cell types and lineages in airway epithelium, including the pulmonary ionocyte, a new cell type predominantly expressing the cystic fibrosis gene CFTR.

    • Daniel T. Montoro
    • , Adam L. Haber
    • , Moshe Biton
    • , Vladimir Vinarsky
    • , Brian Lin
    • , Susan E. Birket
    • , Feng Yuan
    • , Sijia Chen
    • , Hui Min Leung
    • , Jorge Villoria
    • , Noga Rogel
    • , Grace Burgin
    • , Alexander M. Tsankov
    • , Avinash Waghray
    • , Michal Slyper
    • , Julia Waldman
    • , Lan Nguyen
    • , Danielle Dionne
    • , Orit Rozenblatt-Rosen
    • , Purushothama Rao Tata
    • , Hongmei Mou
    • , Manjunatha Shivaraju
    • , Hermann Bihler
    • , Martin Mense
    • , Guillermo J. Tearney
    • , Steven M. Rowe
    • , John F. Engelhardt
    • , Aviv Regev
    •  & Jayaraj Rajagopal
  • Article |

    The extent, origins and consequences of genetic variation within human cell lines are studied, providing a framework for researchers to measure such variation in efforts to support maximally reproducible cancer research.

    • Uri Ben-David
    • , Benjamin Siranosian
    • , Gavin Ha
    • , Helen Tang
    • , Yaara Oren
    • , Kunihiko Hinohara
    • , Craig A. Strathdee
    • , Joshua Dempster
    • , Nicholas J. Lyons
    • , Robert Burns
    • , Anwesha Nag
    • , Guillaume Kugener
    • , Beth Cimini
    • , Peter Tsvetkov
    • , Yosef E. Maruvka
    • , Ryan O’Rourke
    • , Anthony Garrity
    • , Andrew A. Tubelli
    • , Pratiti Bandopadhayay
    • , Aviad Tsherniak
    • , Francisca Vazquez
    • , Bang Wong
    • , Chet Birger
    • , Mahmoud Ghandi
    • , Aaron R. Thorner
    • , Joshua A. Bittker
    • , Matthew Meyerson
    • , Gad Getz
    • , Rameen Beroukhim
    •  & Todd R. Golub
  • Article |

    Successive fusion of yeast chromosomes is used to produce a single-chromosome strain that is viable, albeit with slightly reduced fitness.

    • Yangyang Shao
    • , Ning Lu
    • , Zhenfang Wu
    • , Chen Cai
    • , Shanshan Wang
    • , Ling-Li Zhang
    • , Fan Zhou
    • , Shijun Xiao
    • , Lin Liu
    • , Xiaofei Zeng
    • , Huajun Zheng
    • , Chen Yang
    • , Zhihu Zhao
    • , Guoping Zhao
    • , Jin-Qiu Zhou
    • , Xiaoli Xue
    •  & Zhongjun Qin
  • Letters

  • Letter |

    Two- and three-layer WTe2 exhibits spontaneous out-of-plane electric polarization that can be switched electrically at room temperature and is sufficiently robust for use in applications with other two-dimensional materials.

    • Zaiyao Fei
    • , Wenjin Zhao
    • , Tauno A. Palomaki
    • , Bosong Sun
    • , Moira K. Miller
    • , Zhiying Zhao
    • , Jiaqiang Yan
    • , Xiaodong Xu
    •  & David H. Cobden
  • Letter |

    Combining cycloaddition and carbon–carbon cross-coupling offers a way of simplifying the enantioselective preparation of chemical building blocks, natural products and medicines such as the antipsychotic asenapine.

    • Tie–Gen Chen
    • , Lisa M. Barton
    • , Yutong Lin
    • , Jet Tsien
    • , David Kossler
    • , Iñaki Bastida
    • , Shota Asai
    • , Cheng Bi
    • , Jason S. Chen
    • , Mingde Shan
    • , Hui Fang
    • , Francis G. Fang
    • , Hyeong-wook Choi
    • , Lynn Hawkins
    • , Tian Qin
    •  & Phil S. Baran
  • Letter |

    Satellite observations and Earth system model simulations reveal that marine heatwaves have increased in recent decades and will increase further in terms of frequency, intensity, duration and spatial extent.

    • Thomas L. Frölicher
    • , Erich M. Fischer
    •  & Nicolas Gruber
  • Letter |

    Female zebra finches exhibited categorical perception of colour signals, as they categorized colour stimuli that varied along a continuous scale and showed increased discrimination between colours from opposite sides of the category boundary compared to equally different colours from within a category.

    • Eleanor M. Caves
    • , Patrick A. Green
    • , Matthew N. Zipple
    • , Susan Peters
    • , Sönke Johnsen
    •  & Stephen Nowicki
  • Letter |

    Experimental whole-ecosystem warming treatments of a PiceaSphagnum peat bog reveal the likely phenological consequences of future temperature increases that exceed those of historical climate regimes.

    • Andrew D. Richardson
    • , Koen Hufkens
    • , Thomas Milliman
    • , Donald M. Aubrecht
    • , Morgan E. Furze
    • , Bijan Seyednasrollah
    • , Misha B. Krassovski
    • , John M. Latimer
    • , W. Robert Nettles
    • , Ryan R. Heiderman
    • , Jeffrey M. Warren
    •  & Paul J. Hanson
  • Letter |

    Many small molecules that stimulate oligodendrocyte formation act not through their canonical pathways but by inhibiting enzymes within the cholesterol biosynthesis pathway and thereby inducing the accumulation of 8,9-unsaturated sterols.

    • Zita Hubler
    • , Dharmaraja Allimuthu
    • , Ilya Bederman
    • , Matthew S. Elitt
    • , Mayur Madhavan
    • , Kevin C. Allan
    • , H. Elizabeth Shick
    • , Eric Garrison
    • , Molly T. Karl
    • , Daniel C. Factor
    • , Zachary S. Nevin
    • , Joel L. Sax
    • , Matthew A. Thompson
    • , Yuriy Fedorov
    • , Jing Jin
    • , William K. Wilson
    • , Martin Giera
    • , Franz Bracher
    • , Robert H. Miller
    • , Paul J. Tesar
    •  & Drew J. Adams
  • Letter |

    Single-cell RNA sequencing analysis is used to identify cell types in the tracheal epithelium, including previously unidentified ionocytes, which express high levels of the cystic fibrosis transmembrane conductance regulator, CFTR.

    • Lindsey W. Plasschaert
    • , Rapolas Žilionis
    • , Rayman Choo-Wing
    • , Virginia Savova
    • , Judith Knehr
    • , Guglielmo Roma
    • , Allon M. Klein
    •  & Aron B. Jaffe
  • Letter |

    Melanoma cells release programmed death-ligand 1 (PD-L1) on the surface of circulating exosomes, suggesting a mechanism by which tumours could evade the immunesystem, and the potential application of exosomal PD-L1 to monitor patient responses to checkpoint therapies.

    • Gang Chen
    • , Alexander C. Huang
    • , Wei Zhang
    • , Gao Zhang
    • , Min Wu
    • , Wei Xu
    • , Zili Yu
    • , Jiegang Yang
    • , Beike Wang
    • , Honghong Sun
    • , Houfu Xia
    • , Qiwen Man
    • , Wenqun Zhong
    • , Leonardo F. Antelo
    • , Bin Wu
    • , Xuepeng Xiong
    • , Xiaoming Liu
    • , Lei Guan
    • , Ting Li
    • , Shujing Liu
    • , Ruifeng Yang
    • , Youtao Lu
    • , Liyun Dong
    • , Suzanne McGettigan
    • , Rajasekharan Somasundaram
    • , Ravi Radhakrishnan
    • , Gordon Mills
    • , Yiling Lu
    • , Junhyong Kim
    • , Youhai H. Chen
    • , Haidong Dong
    • , Yifang Zhao
    • , Giorgos C. Karakousis
    • , Tara C. Mitchell
    • , Lynn M. Schuchter
    • , Meenhard Herlyn
    • , E. John Wherry
    • , Xiaowei Xu
    •  & Wei Guo
  • Letter |

    A pro-survival multiprotein signalling supercomplex consisting of the B cell receptor, MYD88, TLR9 and mTOR is discovered that coordinates NF-κB activation in diffuse large B cell lymphoma, and provides mechanistic insight into the efficacy of drug combinations.

    • James D. Phelan
    • , Ryan M. Young
    • , Daniel E. Webster
    • , Sandrine Roulland
    • , George W. Wright
    • , Monica Kasbekar
    • , Arthur L. Shaffer III
    • , Michele Ceribelli
    • , James Q. Wang
    • , Roland Schmitz
    • , Masao Nakagawa
    • , Emmanuel Bachy
    • , Da Wei Huang
    • , Yanlong Ji
    • , Lu Chen
    • , Yandan Yang
    • , Hong Zhao
    • , Xin Yu
    • , Weihong Xu
    • , Maryknoll M. Palisoc
    • , Racquel R. Valadez
    • , Theresa Davies-Hill
    • , Wyndham H. Wilson
    • , Wing C. Chan
    • , Elaine S. Jaffe
    • , Randy D. Gascoyne
    • , Elias Campo
    • , Andreas Rosenwald
    • , German Ott
    • , Jan Delabie
    • , Lisa M. Rimsza
    • , Fausto J. Rodriguez
    • , Fayez Estephan
    • , Matthias Holdhoff
    • , Michael J. Kruhlak
    • , Stephen M. Hewitt
    • , Craig J. Thomas
    • , Stefania Pittaluga
    • , Thomas Oellerich
    •  & Louis M. Staudt

Amendments & Corrections

Newsletter Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing