Abstract
FOUR weeks ago, Dr. G. Millikan brought us some crystals of pepsin prepared by Dr. Philpot in the laboratory of Prof. The Svedberg, Uppsala. They are in the form of perfect hexagonal bipyramids up to 2 mm. in length, of axial ratio c/a = 2.3 ± 0.1. When examined in their mother liquor, they appear moderately birefringent and positively uniaxial, showing a good interference figure. On exposure to air, however, the birefringence rapidly diminishes. X-ray photographs taken of the crystals in the usual way showed nothing but a vague blackening. This indicates complete alteration of the crystal and explains why previous workers have obtained negative results with proteins, so far as crystalline pattern is concerned1. W. T. Astbury has, however, shown that the altered pepsin is a protein of the chain type like myosin or keratin giving an amorphous or fibre pattern.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
G. L. Clark and K. E. Korrigan (Phys. Rev., (ii), 40, 639; 1932) describe long spacings found from crystalline insulin, but no details have been published.
J. H. Northrop, J. Gen. Physiol., 13, 739 ; 1930.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BERNAL, J., CROWFOOT, D. X-Ray Photographs of Crystalline Pepsin. Nature 133, 794–795 (1934). https://doi.org/10.1038/133794b0
Issue Date:
DOI: https://doi.org/10.1038/133794b0
This article is cited by
-
The paths to the atomic structures of proteins and nucleic acids
ChemTexts (2023)
-
How Seeing Became Knowing: The Role of the Electron Microscope in Shaping the Modern Definition of Viruses
Journal of the History of Biology (2019)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.