Articles

  • Article |

    The detection of hypocretin-specific autoreactive CD4+ and CD8+ T cells in patients with narcolepsy reveals the autoimmune aetiology of this disorder.

    • Daniela Latorre
    • , Ulf Kallweit
    • , Eric Armentani
    • , Mathilde Foglierini
    • , Federico Mele
    • , Antonino Cassotta
    • , Sandra Jovic
    • , David Jarrossay
    • , Johannes Mathis
    • , Francesco Zellini
    • , Burkhard Becher
    • , Antonio Lanzavecchia
    • , Ramin Khatami
    • , Mauro Manconi
    • , Mehdi Tafti
    • , Claudio L. Bassetti
    •  & Federica Sallusto
  • Article |

    A high-resolution cryo-electron microscopy structure of a complete Tc holotoxin complex reveals the precise mechanism of Tc toxin assembly, gate opening and release of the cytotoxic enzyme into the translocation channel.

    • Christos Gatsogiannis
    • , Felipe Merino
    • , Daniel Roderer
    • , David Balchin
    • , Evelyn Schubert
    • , Anne Kuhlee
    • , Manajit Hayer-Hartl
    •  & Stefan Raunser
  • Article |

    The tumour microenvironment determines which type of liver cancer develops, with transformed hepatocytes giving rise to intrahepatic cholangiocarcinoma or hepatocellular carcinoma depending or whether they are surrounded by cells undergoing necroptosis or apoptosis.

    • Marco Seehawer
    • , Florian Heinzmann
    • , Luana D’Artista
    • , Jule Harbig
    • , Pierre-François Roux
    • , Lisa Hoenicke
    • , Hien Dang
    • , Sabrina Klotz
    • , Lucas Robinson
    • , Grégory Doré
    • , Nir Rozenblum
    • , Tae-Won Kang
    • , Rishabh Chawla
    • , Thorsten Buch
    • , Mihael Vucur
    • , Mareike Roth
    • , Johannes Zuber
    • , Tom Luedde
    • , Bence Sipos
    • , Thomas Longerich
    • , Mathias Heikenwälder
    • , Xin Wei Wang
    • , Oliver Bischof
    •  & Lars Zender
  • Article |

    Chemical optimization of arylomycins results in an inhibitor of bacterial type I signal peptidase that shows activity both against multidrug-resistant clinical isolates of Gram-negative bacteria in vitro and in several in vivo infection models.

    • Peter A. Smith
    • , Michael F. T. Koehler
    • , Hany S. Girgis
    • , Donghong Yan
    • , Yongsheng Chen
    • , Yuan Chen
    • , James J. Crawford
    • , Matthew R. Durk
    • , Robert I. Higuchi
    • , Jing Kang
    • , Jeremy Murray
    • , Prasuna Paraselli
    • , Summer Park
    • , Wilson Phung
    • , John G. Quinn
    • , Tucker C. Roberts
    • , Lionel Rougé
    • , Jacob B. Schwarz
    • , Elizabeth Skippington
    • , John Wai
    • , Min Xu
    • , Zhiyong Yu
    • , Hua Zhang
    • , Man-Wah Tan
    •  & Christopher E. Heise
  • Article |

    Germline BRCA1 loss-of-function variants are associated with predisposition to early-onset breast and ovarian cancer; here the authors use CRISPR/Cas9 genome editing to functionally assess thousands of BRCA1 variants in order to facilitate the clinical interpretation of these variants.

    • Gregory M. Findlay
    • , Riza M. Daza
    • , Beth Martin
    • , Melissa D. Zhang
    • , Anh P. Leith
    • , Molly Gasperini
    • , Joseph D. Janizek
    • , Xingfan Huang
    • , Lea M. Starita
    •  & Jay Shendure
  • Article |

    The elucidation of general principles for designing β-barrels enables the de novo creation of fluorescent proteins.

    • Jiayi Dou
    • , Anastassia A. Vorobieva
    • , William Sheffler
    • , Lindsey A. Doyle
    • , Hahnbeom Park
    • , Matthew J. Bick
    • , Binchen Mao
    • , Glenna W. Foight
    • , Min Yen Lee
    • , Lauren A. Gagnon
    • , Lauren Carter
    • , Banumathi Sankaran
    • , Sergey Ovchinnikov
    • , Enrique Marcos
    • , Po-Ssu Huang
    • , Joshua C. Vaughan
    • , Barry L. Stoddard
    •  & David Baker
  • Article |

    A large-scale genomics study shows that the cell of origin and founding mutations determine disease subtype and lead to the expression of multiple haematopoietic lineage-defining antigens in mixed phenotype acute leukaemia.

    • Thomas B. Alexander
    • , Zhaohui Gu
    • , Ilaria Iacobucci
    • , Kirsten Dickerson
    • , John K. Choi
    • , Beisi Xu
    • , Debbie Payne-Turner
    • , Hiroki Yoshihara
    • , Mignon L. Loh
    • , John Horan
    • , Barbara Buldini
    • , Giuseppe Basso
    • , Sarah Elitzur
    • , Valerie de Haas
    • , C. Michel Zwaan
    • , Allen Yeoh
    • , Dirk Reinhardt
    • , Daisuke Tomizawa
    • , Nobutaka Kiyokawa
    • , Tim Lammens
    • , Barbara De Moerloose
    • , Daniel Catchpoole
    • , Hiroki Hori
    • , Anthony Moorman
    • , Andrew S. Moore
    • , Ondrej Hrusak
    • , Soheil Meshinchi
    • , Etan Orgel
    • , Meenakshi Devidas
    • , Michael Borowitz
    • , Brent Wood
    • , Nyla A. Heerema
    • , Andrew Carrol
    • , Yung-Li Yang
    • , Malcolm A. Smith
    • , Tanja M. Davidsen
    • , Leandro C. Hermida
    • , Patee Gesuwan
    • , Marco A. Marra
    • , Yussanne Ma
    • , Andrew J. Mungall
    • , Richard A. Moore
    • , Steven J. M. Jones
    • , Marcus Valentine
    • , Laura J. Janke
    • , Jeffrey E. Rubnitz
    • , Ching-Hon Pui
    • , Liang Ding
    • , Yu Liu
    • , Jinghui Zhang
    • , Kim E. Nichols
    • , James R. Downing
    • , Xueyuan Cao
    • , Lei Shi
    • , Stanley Pounds
    • , Scott Newman
    • , Deqing Pei
    • , Jaime M. Guidry Auvil
    • , Daniela S. Gerhard
    • , Stephen P. Hunger
    • , Hiroto Inaba
    •  & Charles G. Mullighan
  • Article |

    The structure of a complex containing calcitonin gene-related peptide, the human calcitonin gene-related peptide receptor and the Gs heterotrimer, determined using Volta phase-plate cryo-electron microscopy, provides structural insight into the regulation of G-protein-coupled receptors by receptor activity modifying protein 1.

    • Yi-Lynn Liang
    • , Maryam Khoshouei
    • , Giuseppe Deganutti
    • , Alisa Glukhova
    • , Cassandra Koole
    • , Thomas S. Peat
    • , Mazdak Radjainia
    • , Jürgen M. Plitzko
    • , Wolfgang Baumeister
    • , Laurence J. Miller
    • , Deborah L. Hay
    • , Arthur Christopoulos
    • , Christopher A. Reynolds
    • , Denise Wootten
    •  & Patrick M. Sexton
  • Article |

    Therapeutic T cells bearing ligands engineered to optimize adhesion and transmigration through the blood–brain barrier can be targeted to brain tumours.

    • Heba Samaha
    • , Antonella Pignata
    • , Kristen Fousek
    • , Jun Ren
    • , Fong W. Lam
    • , Fabio Stossi
    • , Julien Dubrulle
    • , Vita S. Salsman
    • , Shanmugarajan Krishnan
    • , Sung-Ha Hong
    • , Matthew L. Baker
    • , Ankita Shree
    • , Ahmed Z. Gad
    • , Thomas Shum
    • , Dai Fukumura
    • , Tiara T. Byrd
    • , Malini Mukherjee
    • , Sean P. Marrelli
    • , Jordan S. Orange
    • , Sujith K. Joseph
    • , Poul H. Sorensen
    • , Michael D. Taylor
    • , Meenakshi Hegde
    • , Maksim Mamonkin
    • , Rakesh K. Jain
    • , Shahenda El-Naggar
    •  & Nabil Ahmed
  • Article |

    Osteoclasts secrete small extracellular vesicles that stimulate osteoblasts, promoting bone formation via receptor activator of nuclear factor-kappa B ligand (RANKL), thereby linking bone formation and resorption.

    • Yuki Ikebuchi
    • , Shigeki Aoki
    • , Masashi Honma
    • , Madoka Hayashi
    • , Yasutaka Sugamori
    • , Masud Khan
    • , Yoshiaki Kariya
    • , Genki Kato
    • , Yasuhiko Tabata
    • , Josef M. Penninger
    • , Nobuyuki Udagawa
    • , Kazuhiro Aoki
    •  & Hiroshi Suzuki
  • Article |

    Analysis of blood from a healthy human show that haematopoietic stem cells increase rapidly in numbers through early life, reaching a stable plateau in adulthood, and contribute to myeloid and B lymphocyte populations throughout life.

    • Henry Lee-Six
    • , Nina Friesgaard Øbro
    • , Mairi S. Shepherd
    • , Sebastian Grossmann
    • , Kevin Dawson
    • , Miriam Belmonte
    • , Robert J. Osborne
    • , Brian J. P. Huntly
    • , Inigo Martincorena
    • , Elizabeth Anderson
    • , Laura O’Neill
    • , Michael R. Stratton
    • , Elisa Laurenti
    • , Anthony R. Green
    • , David G. Kent
    •  & Peter J. Campbell
  • Article |

    Differential DNA methylation and the long-range effects of chromatin organization lead to pronounced differences in recombination landscape between males and females.

    • Kevin Brick
    • , Sarah Thibault-Sennett
    • , Fatima Smagulova
    • , Kwan-Wood G. Lam
    • , Yongmei Pu
    • , Florencia Pratto
    • , R. Daniel Camerini-Otero
    •  & Galina V. Petukhova
  • Article |

    Comparison of multiple lesions from individual pancreases sheds light on how ancestral clones can spread through the ductal system and give rise to precursor lesions, with acquisition of further mutations leading to pancreatic cancer.

    • Alvin P. Makohon-Moore
    • , Karen Matsukuma
    • , Ming Zhang
    • , Johannes G. Reiter
    • , Jeffrey M. Gerold
    • , Yuchen Jiao
    • , Lisa Sikkema
    • , Marc A. Attiyeh
    • , Shinichi Yachida
    • , Corinne Sandone
    • , Ralph H. Hruban
    • , David S. Klimstra
    • , Nickolas Papadopoulos
    • , Martin A. Nowak
    • , Kenneth W. Kinzler
    • , Bert Vogelstein
    •  & Christine A. Iacobuzio-Donahue
  • Article |

    Temporal information that is useful for episodic memory is encoded across a wide range of timescales in the lateral entorhinal cortex, arising inherently from its representation of ongoing experience.

    • Albert Tsao
    • , Jørgen Sugar
    • , Li Lu
    • , Cheng Wang
    • , James J. Knierim
    • , May-Britt Moser
    •  & Edvard I. Moser
  • Article |

    The enzyme glutamine synthetase is active in endothelial cell migration during angiogenesis, through autopalmitoylation and the regulation of RHOJ signalling.

    • Guy Eelen
    • , Charlotte Dubois
    • , Anna Rita Cantelmo
    • , Jermaine Goveia
    • , Ulrike Brüning
    • , Michael DeRan
    • , Gopala Jarugumilli
    • , Jos van Rijssel
    • , Giorgio Saladino
    • , Federico Comitani
    • , Annalisa Zecchin
    • , Susana Rocha
    • , Rongyuan Chen
    • , Hongling Huang
    • , Saar Vandekeere
    • , Joanna Kalucka
    • , Christian Lange
    • , Francisco Morales-Rodriguez
    • , Bert Cruys
    • , Lucas Treps
    • , Leanne Ramer
    • , Stefan Vinckier
    • , Katleen Brepoels
    • , Sabine Wyns
    • , Joris Souffreau
    • , Luc Schoonjans
    • , Wouter H. Lamers
    • , Yi Wu
    • , Jurgen Haustraete
    • , Johan Hofkens
    • , Sandra Liekens
    • , Richard Cubbon
    • , Bart Ghesquière
    • , Mieke Dewerchin
    • , Francesco L. Gervasio
    • , Xuri Li
    • , Jaap D. van Buul
    • , Xu Wu
    •  & Peter Carmeliet
  • Article |

    Crystal structures and molecular simulations of the designed anion-conducting channelrhodopsin iC++ provide molecular insights that enable structure-based design of channelrhodopsins with desirable properties for use as optogenetic tools.

    • Hideaki E. Kato
    • , Yoon Seok Kim
    • , Joseph M. Paggi
    • , Kathryn E. Evans
    • , William E. Allen
    • , Claire Richardson
    • , Keiichi Inoue
    • , Shota Ito
    • , Charu Ramakrishnan
    • , Lief E. Fenno
    • , Keitaro Yamashita
    • , Daniel Hilger
    • , Soo Yeun Lee
    • , Andre Berndt
    • , Kang Shen
    • , Hideki Kandori
    • , Ron O. Dror
    • , Brian K. Kobilka
    •  & Karl Deisseroth
  • Article |

    The crystal structure of anion channelrhodopsin-1 (ACR1) from the algae Guillardia theta provides insights into the basis of anion conductance.

    • Yoon Seok Kim
    • , Hideaki E. Kato
    • , Keitaro Yamashita
    • , Shota Ito
    • , Keiichi Inoue
    • , Charu Ramakrishnan
    • , Lief E. Fenno
    • , Kathryn E. Evans
    • , Joseph M. Paggi
    • , Ron O. Dror
    • , Hideki Kandori
    • , Brian K. Kobilka
    •  & Karl Deisseroth
  • Article |

    The Newtonian gravitational constant is measured with two independent methods, yielding results with relative standard uncertainties of 11.6 parts per million—the lowest uncertainty reported until now.

    • Qing Li
    • , Chao Xue
    • , Jian-Ping Liu
    • , Jun-Fei Wu
    • , Shan-Qing Yang
    • , Cheng-Gang Shao
    • , Li-Di Quan
    • , Wen-Hai Tan
    • , Liang-Cheng Tu
    • , Qi Liu
    • , Hao Xu
    • , Lin-Xia Liu
    • , Qing-Lan Wang
    • , Zhong-Kun Hu
    • , Ze-Bing Zhou
    • , Peng-Shun Luo
    • , Shu-Chao Wu
    • , Vadim Milyukov
    •  & Jun Luo
  • Article |

    Cryo-electron microscopy analysis of the purified Plasmodium translocon of exported proteins (PTEX) reveals two distinct resolved states, suggesting a mechanism by which Plasmodium falciparum exports malarial effector proteins into erythrocytes.

    • Chi-Min Ho
    • , Josh R. Beck
    • , Mason Lai
    • , Yanxiang Cui
    • , Daniel E. Goldberg
    • , Pascal F. Egea
    •  & Z. Hong Zhou
  • Article |

    The cryo-electron microscopy structure of a paused transcription elongation complex of RNA polymerase II bound to DRB sensitivity-inducing factor and negative elongation factor is reported at 3.2 Å resolution.

    • Seychelle M. Vos
    • , Lucas Farnung
    • , Henning Urlaub
    •  & Patrick Cramer
  • Article |

    The cryo-electron microscopy structure of an activated transcription elongation complex of RNA polymerase II bound to DRB sensitivity-inducing factor and the elongation factors PAF1 complex and SPT6 is reported at 3.1 Å resolution.

    • Seychelle M. Vos
    • , Lucas Farnung
    • , Marc Boehning
    • , Christoph Wigge
    • , Andreas Linden
    • , Henning Urlaub
    •  & Patrick Cramer
  • Article |

    The balance of DELLA and GRF4 proteins in plants ensures the co-regulation of growth with metabolism and tipping this balance towards GRF4 leads to higher efficiency of nitrogen use.

    • Shan Li
    • , Yonghang Tian
    • , Kun Wu
    • , Yafeng Ye
    • , Jianping Yu
    • , Jianqing Zhang
    • , Qian Liu
    • , Mengyun Hu
    • , Hui Li
    • , Yiping Tong
    • , Nicholas P. Harberd
    •  & Xiangdong Fu
  • Article |

    A cryo-electron microscopy structure of the insect Orco subunit, which forms ion channels with diverse olfactory receptors, reveals a tetrameric cation channel and sheds light on insect olfaction.

    • Joel A. Butterwick
    • , Josefina del Mármol
    • , Kelly H. Kim
    • , Martha A. Kahlson
    • , Jackson A. Rogow
    • , Thomas Walz
    •  & Vanessa Ruta
  • Article |

    CPEB4 binds the mRNA of genes known to be associated with autism and shows an isoform imbalance in individuals with autism, and an equivalent imbalance in mice induces an autism-like phenotype.

    • Alberto Parras
    • , Héctor Anta
    • , María Santos-Galindo
    • , Vivek Swarup
    • , Ainara Elorza
    • , José L. Nieto-González
    • , Sara Picó
    • , Ivó H. Hernández
    • , Juan I. Díaz-Hernández
    • , Eulàlia Belloc
    • , Annie Rodolosse
    • , Neelroop N. Parikshak
    • , Olga Peñagarikano
    • , Rafael Fernández-Chacón
    • , Manuel Irimia
    • , Pilar Navarro
    • , Daniel H. Geschwind
    • , Raúl Méndez
    •  & José J. Lucas
  • Article |

    The extent, origins and consequences of genetic variation within human cell lines are studied, providing a framework for researchers to measure such variation in efforts to support maximally reproducible cancer research.

    • Uri Ben-David
    • , Benjamin Siranosian
    • , Gavin Ha
    • , Helen Tang
    • , Yaara Oren
    • , Kunihiko Hinohara
    • , Craig A. Strathdee
    • , Joshua Dempster
    • , Nicholas J. Lyons
    • , Robert Burns
    • , Anwesha Nag
    • , Guillaume Kugener
    • , Beth Cimini
    • , Peter Tsvetkov
    • , Yosef E. Maruvka
    • , Ryan O’Rourke
    • , Anthony Garrity
    • , Andrew A. Tubelli
    • , Pratiti Bandopadhayay
    • , Aviad Tsherniak
    • , Francisca Vazquez
    • , Bang Wong
    • , Chet Birger
    • , Mahmoud Ghandi
    • , Aaron R. Thorner
    • , Joshua A. Bittker
    • , Matthew Meyerson
    • , Gad Getz
    • , Rameen Beroukhim
    •  & Todd R. Golub
  • Article |

    Stimulating the release of serotonin (5-HT) in the nucleus accumbens in wild-type mice promotes sociability, and rescues deficits in social behaviours in a mouse model of autism.

    • Jessica J. Walsh
    • , Daniel J. Christoffel
    • , Boris D. Heifets
    • , Gabriel A. Ben-Dor
    • , Aslihan Selimbeyoglu
    • , Lin W. Hung
    • , Karl Deisseroth
    •  & Robert C. Malenka
  • Article |

    Successive fusion of yeast chromosomes is used to produce a single-chromosome strain that is viable, albeit with slightly reduced fitness.

    • Yangyang Shao
    • , Ning Lu
    • , Zhenfang Wu
    • , Chen Cai
    • , Shanshan Wang
    • , Ling-Li Zhang
    • , Fan Zhou
    • , Shijun Xiao
    • , Lin Liu
    • , Xiaofei Zeng
    • , Huajun Zheng
    • , Chen Yang
    • , Zhihu Zhao
    • , Guoping Zhao
    • , Jin-Qiu Zhou
    • , Xiaoli Xue
    •  & Zhongjun Qin
  • Article |

    Single-cell RNA sequencing analysis identifies cell types and lineages in airway epithelium, including the pulmonary ionocyte, a new cell type predominantly expressing the cystic fibrosis gene CFTR.

    • Daniel T. Montoro
    • , Adam L. Haber
    • , Moshe Biton
    • , Vladimir Vinarsky
    • , Brian Lin
    • , Susan E. Birket
    • , Feng Yuan
    • , Sijia Chen
    • , Hui Min Leung
    • , Jorge Villoria
    • , Noga Rogel
    • , Grace Burgin
    • , Alexander M. Tsankov
    • , Avinash Waghray
    • , Michal Slyper
    • , Julia Waldman
    • , Lan Nguyen
    • , Danielle Dionne
    • , Orit Rozenblatt-Rosen
    • , Purushothama Rao Tata
    • , Hongmei Mou
    • , Manjunatha Shivaraju
    • , Hermann Bihler
    • , Martin Mense
    • , Guillermo J. Tearney
    • , Steven M. Rowe
    • , John F. Engelhardt
    • , Aviv Regev
    •  & Jayaraj Rajagopal
  • Article |

    Micrometre-sized particles of two niobium tungsten oxides have high volumetric capacities and rate performances, enabled by very high lithium-ion diffusion coefficients.

    • Kent J. Griffith
    • , Kamila M. Wiaderek
    • , Giannantonio Cibin
    • , Lauren E. Marbella
    •  & Clare P. Grey
  • Article |

    Meningeal lymphatic dysfunction promotes amyloid-β deposition in the meninges and worsens brain amyloid-β pathology, acting as an aggravating factor in Alzheimer’s disease and in age-associated cognitive decline; improving meningeal lymphatic function could help to prevent or delay age-associated neurological diseases.

    • Sandro Da Mesquita
    • , Antoine Louveau
    • , Andrea Vaccari
    • , Igor Smirnov
    • , R. Chase Cornelison
    • , Kathryn M. Kingsmore
    • , Christian Contarino
    • , Suna Onengut-Gumuscu
    • , Emily Farber
    • , Daniel Raper
    • , Kenneth E. Viar
    • , Romie D. Powell
    • , Wendy Baker
    • , Nisha Dabhi
    • , Robin Bai
    • , Rui Cao
    • , Song Hu
    • , Stephen S. Rich
    • , Jennifer M. Munson
    • , M. Beatriz Lopes
    • , Christopher C. Overall
    • , Scott T. Acton
    •  & Jonathan Kipnis
  • Article |

    Coherent coupling between a three-electron spin qubit and single photons in a microwave resonator is demonstrated, which, unlike previous demonstrations, does not require ferromagnetic components near the qubit.

    • A. J. Landig
    • , J. V. Koski
    • , P. Scarlino
    • , U. C. Mendes
    • , A. Blais
    • , C. Reichl
    • , W. Wegscheider
    • , A. Wallraff
    • , K. Ensslin
    •  & T. Ihn
  • Article |

    New mitochondrial DNA synthesis links the priming and activation of the NLRP3 inflammasome.

    • Zhenyu Zhong
    • , Shuang Liang
    • , Elsa Sanchez-Lopez
    • , Feng He
    • , Shabnam Shalapour
    • , Xue-jia Lin
    • , Jerry Wong
    • , Siyuan Ding
    • , Ekihiro Seki
    • , Bernd Schnabl
    • , Andrea L. Hevener
    • , Harry B. Greenberg
    • , Tatiana Kisseleva
    •  & Michael Karin
  • Article |

    A series of compounds are discovered for the treatment of visceral leishmaniasis, and cdc2-related kinase 12 (CRK12) is identified as the probable primary drug target.

    • Susan Wyllie
    • , Michael Thomas
    • , Stephen Patterson
    • , Sabrinia Crouch
    • , Manu De Rycker
    • , Rhiannon Lowe
    • , Stephanie Gresham
    • , Michael D. Urbaniak
    • , Thomas D. Otto
    • , Laste Stojanovski
    • , Frederick R. C. Simeons
    • , Sujatha Manthri
    • , Lorna M. MacLean
    • , Fabio Zuccotto
    • , Nadine Homeyer
    • , Hannah Pflaumer
    • , Markus Boesche
    • , Lalitha Sastry
    • , Paul Connolly
    • , Sebastian Albrecht
    • , Matt Berriman
    • , Gerard Drewes
    • , David W. Gray
    • , Sonja Ghidelli-Disse
    • , Susan Dixon
    • , Jose M. Fiandor
    • , Paul G. Wyatt
    • , Michael A. J. Ferguson
    • , Alan H. Fairlamb
    • , Timothy J. Miles
    • , Kevin D. Read
    •  & Ian H. Gilbert
  • Article |

    Expression of α6 integrin enables acute lymphoblastic leukaemia cells to use neural migratory pathways to invade the central nervous system and metastasize to the brain.

    • Hisayuki Yao
    • , Trevor T. Price
    • , Gaia Cantelli
    • , Brandon Ngo
    • , Matthew J. Warner
    • , Lindsey Olivere
    • , Sarah M. Ridge
    • , Elizabeth M. Jablonski
    • , Joseph Therrien
    • , Stacey Tannheimer
    • , Chad M. McCall
    • , Anjen Chenn
    •  & Dorothy A. Sipkins
  • Article |

    Combining an electron microscope pixel-array detector that collects the entire distribution of scattered electrons with full-field ptychography greatly improves image resolution and contrast compared to traditional techniques, even at low beam energies.

    • Yi Jiang
    • , Zhen Chen
    • , Yimo Han
    • , Pratiti Deb
    • , Hui Gao
    • , Saien Xie
    • , Prafull Purohit
    • , Mark W. Tate
    • , Jiwoong Park
    • , Sol M. Gruner
    • , Veit Elser
    •  & David A. Muller
  • Article |

    Analysis of more than 1,500 microbial genomes sheds light on the processing of carbon released as permafrost thaws.

    • Ben J. Woodcroft
    • , Caitlin M. Singleton
    • , Joel A. Boyd
    • , Paul N. Evans
    • , Joanne B. Emerson
    • , Ahmed A. F. Zayed
    • , Robert D. Hoelzle
    • , Timothy O. Lamberton
    • , Carmody K. McCalley
    • , Suzanne B. Hodgkins
    • , Rachel M. Wilson
    • , Samuel O. Purvine
    • , Carrie D. Nicora
    • , Changsheng Li
    • , Steve Frolking
    • , Jeffrey P. Chanton
    • , Patrick M. Crill
    • , Scott R. Saleska
    • , Virginia I. Rich
    •  & Gene W. Tyson
  • Article |

    X-ray and cryo-electron microscopy structures of fungal mitochondrial calcium uniporter proteins reveal a tetrameric architecture and shed light on the function of the channel.

    • Chao Fan
    • , Minrui Fan
    • , Benjamin J. Orlando
    • , Nathan M. Fastman
    • , Jinru Zhang
    • , Yan Xu
    • , Melissa G. Chambers
    • , Xiaofang Xu
    • , Kay Perry
    • , Maofu Liao
    •  & Liang Feng
  • Article |

    A cryo-electron microscopy structure of fungal mitochondrial calcium uniporter shows that the channel is tetrameric and sheds light on channel assembly and function.

    • Nam X. Nguyen
    • , Jean-Paul Armache
    • , Changkeun Lee
    • , Yi Yang
    • , Weizhong Zeng
    • , Vamsi K. Mootha
    • , Yifan Cheng
    • , Xiao-chen Bai
    •  & Youxing Jiang
  • Article |

    A female Drosophila melanogaster pheromone is recognized by males from both the same and a closely related species through conserved peripheral sensory neurons; the signal is then differentially propagated to promote conspecific and suppress interspecies courtship.

    • Laura F. Seeholzer
    • , Max Seppo
    • , David L. Stern
    •  & Vanessa Ruta
  • Article |

    Analysis of genotyping data for more than 150,000 individuals from the UK Biobank using long-range phase information sheds light on mechanisms of clonal haematopoiesis.

    • Po-Ru Loh
    • , Giulio Genovese
    • , Robert E. Handsaker
    • , Hilary K. Finucane
    • , Yakir A. Reshef
    • , Pier Francesco Palamara
    • , Brenda M. Birmann
    • , Michael E. Talkowski
    • , Samuel F. Bakhoum
    • , Steven A. McCarroll
    •  & Alkes L. Price
  • Article |

    The cryo-EM structure of the yeast origin recognition complex (ORC) bound to a 72-base-pair origin DNA sequence provides insights into the basis of the origin selection mechanism.

    • Ningning Li
    • , Wai Hei Lam
    • , Yuanliang Zhai
    • , Jiaxuan Cheng
    • , Erchao Cheng
    • , Yongqian Zhao
    • , Ning Gao
    •  & Bik-Kwoon Tye
  • Article |

    During development, new arteries can arise from pre-existing veins; the cell fate switch involved occurs gradually and before the onset of blood flow in mouse embryo hearts.

    • Tianying Su
    • , Geoff Stanley
    • , Rahul Sinha
    • , Gaetano D’Amato
    • , Soumya Das
    • , Siyeon Rhee
    • , Andrew H. Chang
    • , Aruna Poduri
    • , Brian Raftrey
    • , Thanh Theresa Dinh
    • , Walter A. Roper
    • , Guang Li
    • , Kelsey E. Quinn
    • , Kathleen M. Caron
    • , Sean Wu
    • , Lucile Miquerol
    • , Eugene C. Butcher
    • , Irving Weissman
    • , Stephen Quake
    •  & Kristy Red-Horse
  • Article |

    The cryo-electron microscopy structure of the type A GABA receptor bound to GABA and the benzodiazepine site antagonist flumazenil reveals structural mechanisms that underlie intersubunit interactions and ligand selectivity of the receptor.

    • Shaotong Zhu
    • , Colleen M. Noviello
    • , Jinfeng Teng
    • , Richard M. Walsh Jr
    • , Jeong Joo Kim
    •  & Ryan E. Hibbs
  • Article |

    IL-23 produced by myeloid-derived suppressor cells regulates castration resistance in prostate cancer by sustaining androgen receptor signalling.

    • Arianna Calcinotto
    • , Clarissa Spataro
    • , Elena Zagato
    • , Diletta Di Mitri
    • , Veronica Gil
    • , Mateus Crespo
    • , Gaston De Bernardis
    • , Marco Losa
    • , Michela Mirenda
    • , Emiliano Pasquini
    • , Andrea Rinaldi
    • , Semini Sumanasuriya
    • , Maryou B. Lambros
    • , Antje Neeb
    • , Roberta Lucianò
    • , Carlo A. Bravi
    • , Daniel Nava-Rodrigues
    • , David Dolling
    • , Tommaso Prayer-Galetti
    • , Ana Ferreira
    • , Alberto Briganti
    • , Antonio Esposito
    • , Simon Barry
    • , Wei Yuan
    • , Adam Sharp
    • , Johann de Bono
    •  & Andrea Alimonti
  • Article |

    The cryo-electron microscopy structure of the human adenosine A1 receptor in complex with adenosine and heterotrimeric Gi2 protein provides molecular insights into receptor and G-protein selectivity.

    • Christopher J. Draper-Joyce
    • , Maryam Khoshouei
    • , David M. Thal
    • , Yi-Lynn Liang
    • , Anh T. N. Nguyen
    • , Sebastian G. B. Furness
    • , Hariprasad Venugopal
    • , Jo-Anne Baltos
    • , Jürgen M. Plitzko
    • , Radostin Danev
    • , Wolfgang Baumeister
    • , Lauren T. May
    • , Denise Wootten
    • , Patrick M. Sexton
    • , Alisa Glukhova
    •  & Arthur Christopoulos
  • Article |

    Polymerization of actin in the cell nucleus, promoted by the ARP2/3 complex, drives the clustering of double-strand DNA breaks into nuclear compartments where they can undergo homology-directed repair.

    • Benjamin R. Schrank
    • , Tomas Aparicio
    • , Yinyin Li
    • , Wakam Chang
    • , Brian T. Chait
    • , Gregg G. Gundersen
    • , Max E. Gottesman
    •  & Jean Gautier
  • Article |

    Relocalization of heterochromatic double-strand breaks to the nuclear periphery in Drosophila cells occurs via directed motions driven by nuclear actin filaments and myosins activated by the Smc5/6 complex.

    • Christopher P. Caridi
    • , Carla D’Agostino
    • , Taehyun Ryu
    • , Grzegorz Zapotoczny
    • , Laetitia Delabaere
    • , Xiao Li
    • , Varandt Y. Khodaverdian
    • , Nuno Amaral
    • , Emily Lin
    • , Alesandra R. Rau
    •  & Irene Chiolo
  • Article |

    Analyses of current coral reef growth rates in the tropical western Atlantic and Indian Ocean show that few reefs will have the capacity to track sea-level rise projections under Representative Concentration Pathway scenarios without sustained ecological recovery.

    • Chris T. Perry
    • , Lorenzo Alvarez-Filip
    • , Nicholas A. J. Graham
    • , Peter J. Mumby
    • , Shaun K. Wilson
    • , Paul S. Kench
    • , Derek P. Manzello
    • , Kyle M. Morgan
    • , Aimee B. A. Slangen
    • , Damian P. Thomson
    • , Fraser Januchowski-Hartley
    • , Scott G. Smithers
    • , Robert S. Steneck
    • , Renee Carlton
    • , Evan N. Edinger
    • , Ian C. Enochs
    • , Nuria Estrada-Saldívar
    • , Michael D. E. Haywood
    • , Graham Kolodziej
    • , Gary N. Murphy
    • , Esmeralda Pérez-Cervantes
    • , Adam Suchley
    • , Lauren Valentino
    • , Robert Boenish
    • , Margaret Wilson
    •  & Chancey Macdonald