Reactive oxygen species (ROS) have important roles in health and disease, but are chemically complex and difficult to measure accurately. This consensus statement proposes guidelines and best practices on the nomenclature and assessment of ROS, oxidative reactions and oxidative damage in cells, tissues and in vivo.

Steuernagel and Lam et al. present HypoMap, an integrated reference atlas of the murine hypothalamus based on 384,925 hypothalamic cells from publicly available single-cell sequencing datasets.

In our tenth instalment of Career pathways, Jin Zhang and Christiane Wrann reflect on their journeys to becoming PIs and the importance of having a vision for your research, finding the right team, and sharing the joy of science through the training of students.

Nature Metabolism is launching an online collection with articles highlighting best practices in experimental design, analysis and reporting to support the metabolic research community and increase the reproducibility of research in the life sciences.

Shin et al. gain structural insight into how pyrimidine synthesis is coupled to cell cycle regulation through the modulation of CAD activity, and identify allostery as a major means to regulate de novo pyrimidine biosynthesis during the mammalian cell cycle.

Dai et al. show that in response to low glucose levels, AMP-activated protein kinase-mediated phosphorylation of WDR24, a component of the GATOR2 complex, leads to suppression of mTORC1 activation.

Retinal pigment epithelial cells are identified as a local source of insulin in the retina, which is stimulated by phagocytosis of photoreceptor outer segments and starvation and has the potential to influence retinal physiology and disease.

Prolonged exposure to acid is shown to reprogram T cell intracellular methionine metabolism to preserve T cell stemness and increase anti-tumor efficacy.

Coral et al. characterize genetically determined discordance between obesity and type 2 diabetes, identifying discordant genes that may convey protection against type 2 diabetes in obesity.

The authors combine multi-layered omics with clinical and biochemical features from individuals affected with methylmalonic aciduria, a rare inherited disease affecting succinyl-CoA synthesis, revealing that anaplerotic rewiring is a targetable feature.

The environment experienced by an individual early in life has long-term health consequences. Here we summarize key factors that should be considered when designing studies in rodents that aim to address the developmental programming of metabolic disease.

Work in non-mammalian model organisms has shed light on many fundamental biological processes. At Nature Metabolism, we are interested in studies that make use of the unique advantages of these models to unravel new insights into metabolic biology.