Latest Research

  • Article |

    Methotrexate-loaded tumour-cell-derived microvesicles induce neutrophil-mediated antitumour activity and can relieve biliary obstructions in patients with extrahepatic cholangiocarcinoma.

    • Yunfeng Gao
    • , Hui Zhang
    • , Nannan Zhou
    • , Pingwei Xu
    • , Jianxiong Wang
    • , Yuan Gao
    • , Xun Jin
    • , Xiaoyu Liang
    • , Jiadi Lv
    • , Yamin Zhang
    • , Ke Tang
    • , Jingwei Ma
    • , Huafeng Zhang
    • , Jing Xie
    • , Fang Yao
    • , Weimin Tong
    • , Yuying Liu
    • , Ximo Wang
    •  & Bo Huang
  • Article |

    Cell-laden hydrogels can be patterned with algorithmically generated sacrificial dendritic vessel networks made of laser-sintered carbohydrate powders.

    • Ian S. Kinstlinger
    • , Sarah H. Saxton
    • , Gisele A. Calderon
    • , Karen Vasquez Ruiz
    • , David R. Yalacki
    • , Palvasha R. Deme
    • , Jessica E. Rosenkrantz
    • , Jesse D. Louis-Rosenberg
    • , Fredrik Johansson
    • , Kevin D. Janson
    • , Daniel W. Sazer
    • , Saarang S. Panchavati
    • , Karl-Dimiter Bissig
    • , Kelly R. Stevens
    •  & Jordan S. Miller
  • Article |

    A method for the multimodal volumetric imaging of RNAs and proteins in intact tumour volumes and organoids enables the spatial transcriptional profiling of coding RNAs and non-coding RNAs at single-cell resolution in cancer tissues.

    • Nobuyuki Tanaka
    • , Shigeaki Kanatani
    • , Dagmara Kaczynska
    • , Keishiro Fukumoto
    • , Lauri Louhivuori
    • , Tomohiro Mizutani
    • , Oded Kopper
    • , Pauliina Kronqvist
    • , Stephanie Robertson
    • , Claes Lindh
    • , Lorand Kis
    • , Robin Pronk
    • , Naoya Niwa
    • , Kazuhiro Matsumoto
    • , Mototsugu Oya
    • , Ayako Miyakawa
    • , Anna Falk
    • , Johan Hartman
    • , Cecilia Sahlgren
    • , Hans Clevers
    •  & Per Uhlén
  • Article |

    The combination of tumour-infarction therapy and chemotherapy, delivered via nanoparticles decorated with a tumour-homing peptide and encapsulating thrombin and doxorubicin, outperforms the corresponding monotherapies in tumour-bearing mice and rabbits.

    • Suping Li
    • , Yinlong Zhang
    • , Shih-Hsin Ho
    • , Bozhao Li
    • , Meifang Wang
    • , Xiongwei Deng
    • , Na Yang
    • , Guangna Liu
    • , Zefang Lu
    • , Junchao Xu
    • , Quanwei Shi
    • , Jing-Yan Han
    • , Lirong Zhang
    • , Yan Wu
    • , Yuliang Zhao
    •  & Guangjun Nie
  • Article |

    Label-free imaging of the endogenous pigment lipofuscin at near-infrared and shortwave-infrared wavelengths enables the longitudinal monitoring of liver injury in mice and in biopsied human livers.

    • Mari Saif
    • , Wilhelmus J. Kwanten
    • , Jessica A. Carr
    • , Ivy X. Chen
    • , Jessica M. Posada
    • , Amitabh Srivastava
    • , Juanye Zhang
    • , Yi Zheng
    • , Matthias Pinter
    • , Sampurna Chatterjee
    • , Samir Softic
    • , C. Ronald Kahn
    • , Klaus van Leyen
    • , Oliver T. Bruns
    • , Rakesh K. Jain
    •  & Moungi G. Bawendi
  • Article |

    A workflow that segments anatomical structures in slit-lamp images and that annotates pathological features in each image improves the performance of a deep-learning algorithm for the diagnosis of ophthalmic disorders.

    • Wangting Li
    • , Yahan Yang
    • , Kai Zhang
    • , Erping Long
    • , Lin He
    • , Lei Zhang
    • , Yi Zhu
    • , Chuan Chen
    • , Zhenzhen Liu
    • , Xiaohang Wu
    • , Dongyuan Yun
    • , Jian Lv
    • , Yizhi Liu
    • , Xiyang Liu
    •  & Haotian Lin
  • Article |

    Three-dimensional cell-laden photosensitive polymer hydrogels can be bioprinted in tissues of live animals, by bio-orthogonal two-photon cycloaddition and crosslinking of the polymers.

    • Anna Urciuolo
    • , Ilaria Poli
    • , Luca Brandolino
    • , Paolo Raffa
    • , Valentina Scattolini
    • , Cecilia Laterza
    • , Giovanni G. Giobbe
    • , Elisa Zambaiti
    • , Giulia Selmin
    • , Michael Magnussen
    • , Laura Brigo
    • , Paolo De Coppi
    • , Stefano Salmaso
    • , Monica Giomo
    •  & Nicola Elvassore
  • Article |

    The travelling kinetics of single cells loaded with mesoporous silica nanoparticles concentrating the 68Ga radioisotope can be tracked in real time in vivo from the patterns of coincident gamma rays detected by positron emission tomography.

    • Kyung Oh Jung
    • , Tae Jin Kim
    • , Jung Ho Yu
    • , Siyeon Rhee
    • , Wei Zhao
    • , Byunghang Ha
    • , Kristy Red-Horse
    • , Sanjiv Sam Gambhir
    •  & Guillem Pratx
  • Article |

    Thousands of individual gastrointestinal organoids cultured on microcavity arrays without a solid extracellular matrix allow for high-throughput drug screening and for high-content image-based phenotypic analyses.

    • Nathalie Brandenberg
    • , Sylke Hoehnel
    • , Fabien Kuttler
    • , Krisztian Homicsko
    • , Camilla Ceroni
    • , Till Ringel
    • , Nikolce Gjorevski
    • , Gerald Schwank
    • , George Coukos
    • , Gerardo Turcatti
    •  & Matthias P. Lutolf
  • Article |

    Buckling kirigami structures applied to footwear outsoles generate higher friction forces transversally to the direction of movement.

    • Sahab Babaee
    • , Simo Pajovic
    • , Ahmad Rafsanjani
    • , Yichao Shi
    • , Katia Bertoldi
    •  & Giovanni Traverso
  • Article |

    A custom-made fidget spinner rapidly concentrates pathogens in 1-ml samples of undiluted urine by more than 100-fold for the on-device colorimetric detection of bacterial load and pathogen identification.

    • Issac Michael
    • , Dongyoung Kim
    • , Oleksandra Gulenko
    • , Sumit Kumar
    • , Saravana Kumar
    • , Jothi Clara
    • , Dong Yeob Ki
    • , Juhee Park
    • , Hyun Yong Jeong
    • , Taek Soo Kim
    • , Sunghoon Kwon
    •  & Yoon-Kyoung Cho
  • Article |

    The co-administration of insulin and pramlintide stabilized with cucurbit[7]uril-conjugated polyethylene glycol in diabetic pigs improves the mealtime suppression of glucagon over the separate administration of the two hormones.

    • Caitlin L. Maikawa
    • , Anton A. A. Smith
    • , Lei Zou
    • , Gillie A. Roth
    • , Emily C. Gale
    • , Lyndsay M. Stapleton
    • , Sam W. Baker
    • , Joseph L. Mann
    • , Anthony C. Yu
    • , Santiago Correa
    • , Abigail K. Grosskopf
    • , Celine S. Liong
    • , Catherine M. Meis
    • , Doreen Chan
    • , Megan Troxell
    • , David M. Maahs
    • , Bruce A. Buckingham
    • , Matthew J. Webber
    •  & Eric A. Appel
  • Publisher Correction |

    • Seung-min Park
    • , Daeyoun D. Won
    • , Brian J. Lee
    • , Diego Escobedo
    • , Andre Esteva
    • , Amin Aalipour
    • , T. Jessie Ge
    • , Jung Ha Kim
    • , Susie Suh
    • , Elliot H. Choi
    • , Alexander X. Lozano
    • , Chengyang Yao
    • , Sunil Bodapati
    • , Friso B. Achterberg
    • , Jeesu Kim
    • , Hwan Park
    • , Youngjae Choi
    • , Woo Jin Kim
    • , Jung Ho Yu
    • , Alexander M. Bhatt
    • , Jong Kyun Lee
    • , Ryan Spitler
    • , Shan X. Wang
    •  & Sanjiv S. Gambhir
  • Article |

    A robotically handled culture system using porcine gastrointestinal tissue explants for the high-throughput interrogation of the gastrointestinal tract predicts the absorption of oral drugs in the human gut better than Caco-2 Transwells.

    • Thomas von Erlach
    • , Sarah Saxton
    • , Yunhua Shi
    • , Daniel Minahan
    • , Daniel Reker
    • , Farhad Javid
    • , Young-Ah Lucy Lee
    • , Carl Schoellhammer
    • , Tina Esfandiary
    • , Cody Cleveland
    • , Lucas Booth
    • , Jiaqi Lin
    • , Hannah Levy
    • , Sophie Blackburn
    • , Alison Hayward
    • , Robert Langer
    •  & Giovanni Traverso
  • Article |

    A plasmonic nanoscale construct serving as a customizable ‘add-on’ fluorescent label improves the signal-to-noise ratio and dynamic range of a range of fluorescence-based bioassays.

    • Jingyi Luan
    • , Anushree Seth
    • , Rohit Gupta
    • , Zheyu Wang
    • , Priya Rathi
    • , Sisi Cao
    • , Hamed Gholami Derami
    • , Rui Tang
    • , Baogang Xu
    • , Samuel Achilefu
    • , Jeremiah J. Morrissey
    •  & Srikanth Singamaneni
  • Article |

    Cardiac organoids incorporating an oxygen-diffusion gradient and stimulated with the neurotransmitter noradrenaline can model the structure and function of the human heart after myocardial infarction.

    • Dylan J. Richards
    • , Yang Li
    • , Charles M. Kerr
    • , Jenny Yao
    • , Gyda C. Beeson
    • , Robert C. Coyle
    • , Xun Chen
    • , Jia Jia
    • , Brooke Damon
    • , Robert Wilson
    • , E. Starr Hazard
    • , Gary Hardiman
    • , Donald R. Menick
    • , Craig C. Beeson
    • , Hai Yao
    • , Tong Ye
    •  & Ying Mei
  • Article |

    A fast and inexpensive point-of-care assay based on CRISPR–Cas13 accurately detects the DNA of opportunistic viruses in blood and urine samples as well as an mRNA marker of renal transplant rejection in urine samples.

    • Michael M. Kaminski
    • , Miguel A. Alcantar
    • , Isadora T. Lape
    • , Robert Greensmith
    • , Allison C. Huske
    • , Jacqueline A. Valeri
    • , Francisco M. Marty
    • , Verena Klämbt
    • , Jamil Azzi
    • , Enver Akalin
    • , Leonardo V. Riella
    •  & James J. Collins
  • Article |

    A collagen-binding interleukin-12 formulation intravenously injected into mice bearing established immunologically cold mouse tumours led to marked tumour remission, particularly when combined with checkpoint-inhibitor immunotherapy.

    • Aslan Mansurov
    • , Jun Ishihara
    • , Peyman Hosseinchi
    • , Lambert Potin
    • , Tiffany M. Marchell
    • , Ako Ishihara
    • , John-Michael Williford
    • , Aaron T. Alpar
    • , Michal M. Raczy
    • , Laura T. Gray
    • , Melody A. Swartz
    •  & Jeffrey A. Hubbell
  • Review Article |

    This Review summarizes progress in the development of engineering strategies employed in reproductive science and medicine, with a focus on biomaterials and microfluidic approaches.

    • Emma S. Gargus
    • , Hunter B. Rogers
    • , Kelly E. McKinnon
    • , Maxwell E. Edmonds
    •  & Teresa K. Woodruff
  • Article |

    A ‘smart’ toilet that uses pressure and motion sensors, biometric identification, urinalysis strips, a computer-vision uroflowmeter and machine learning longitudinally tracks biomarkers of health and disease in the user’s urine and stool.

    • Seung-min Park
    • , Daeyoun D. Won
    • , Brian J. Lee
    • , Diego Escobedo
    • , Andre Esteva
    • , Amin Aalipour
    • , T. Jessie Ge
    • , Jung Ha Kim
    • , Susie Suh
    • , Elliot H. Choi
    • , Alexander X. Lozano
    • , Chengyang Yao
    • , Sunil Bodapati
    • , Friso B. Achterberg
    • , Jeesu Kim
    • , Hwan Park
    • , Youngjae Choi
    • , Woo Jin Kim
    • , Jung Ho Yu
    • , Alexander M. Bhatt
    • , Jong Kyun Lee
    • , Ryan Spitler
    • , Shan X. Wang
    •  & Sanjiv S. Gambhir
  • Article |

    A retrievable, porous and anti-fibrotic macrodevice for the encapsulation of cells provides long-term protection to human cells expressing therapeutic proteins after device implantation in the intraperitoneal space of immunocompetent mice.

    • Suman Bose
    • , Lisa R. Volpatti
    • , Devina Thiono
    • , Volkan Yesilyurt
    • , Collin McGladrigan
    • , Yaoyu Tang
    • , Amanda Facklam
    • , Amy Wang
    • , Siddharth Jhunjhunwala
    • , Omid Veiseh
    • , Jennifer Hollister-Lock
    • , Chandrabali Bhattacharya
    • , Gordon C. Weir
    • , Dale L. Greiner
    • , Robert Langer
    •  & Daniel G. Anderson
  • Article |

    Intravenously injected functionalized carbon quantum dots that bind to the large neutral amino acid transporter 1 and that structurally mimic large amino acids selectively accumulate in human tumours in mice, facilitating targeted theranostics.

    • Shuhua Li
    • , Wen Su
    • , Hao Wu
    • , Ting Yuan
    • , Chang Yuan
    • , Jun Liu
    • , Gang Deng
    • , Xingchun Gao
    • , Zeming Chen
    • , Youmei Bao
    • , Fanglong Yuan
    • , Shixin Zhou
    • , Hongwei Tan
    • , Yunchao Li
    • , Xiaohong Li
    • , Louzhen Fan
    • , Jia Zhu
    • , Ann T. Chen
    • , Fuyao Liu
    • , Yu Zhou
    • , Miao Li
    • , Xingchen Zhai
    •  & Jiangbing Zhou
  • Article |

    A fluorescently labelled inhibitor of the DNA repair enzyme poly(ADP–ribose) polymerase 1 is used to detect epithelial cancers of the upper intestinal tract in animals, in biopsied human tissue and in a human patient.

    • Susanne Kossatz
    • , Giacomo Pirovano
    • , Paula Demétrio De Souza França
    • , Arianna L. Strome
    • , Sumsum P. Sunny
    • , Daniella Karassawa Zanoni
    • , Audrey Mauguen
    • , Brandon Carney
    • , Christian Brand
    • , Veer Shah
    • , Ravindra D. Ramanajinappa
    • , Naveen Hedne
    • , Praveen Birur
    • , Smita Sihag
    • , Ronald A. Ghossein
    • , Mithat Gönen
    • , Marshall Strome
    • , Amritha Suresh
    • , Daniela Molena
    • , Ian Ganly
    • , Moni A. Kuriakose
    • , Snehal G. Patel
    •  & Thomas Reiner
  • Article |

    High-resolution raster-scanning optoacoustic mesoscopy provides detailed morphological and physiological insights into the responses of tumours to vascular-targeted therapies.

    • Katja Haedicke
    • , Lilach Agemy
    • , Murad Omar
    • , Andrei Berezhnoi
    • , Sheryl Roberts
    • , Camila Longo-Machado
    • , Magdalena Skubal
    • , Karan Nagar
    • , Hsiao-Ting Hsu
    • , Kwanghee Kim
    • , Thomas Reiner
    • , Jonathan Coleman
    • , Vasilis Ntziachristos
    • , Avigdor Scherz
    •  & Jan Grimm
  • Article |

    A cyclic octapeptide labelled with a near-infrared dye and that binds, with high affinity at high levels of calcium, to phosphorylated protein Annexin A2 in a range of solid tumours, serves as a tumour-selective imaging probe.

    • Duanwen Shen
    • , Baogang Xu
    • , Kexian Liang
    • , Rui Tang
    • , Gail P. Sudlow
    • , Christopher Egbulefu
    • , Kevin Guo
    • , Avik Som
    • , Rebecca Gilson
    • , Dolonchampa Maji
    • , Suman Mondal
    • , LeMoyne Habimana-Griffin
    • , Walter J. Akers
    • , Shunqiang Li
    • , Yang Liu
    • , Sharon Bloch
    • , Sid Kurkure
    • , Zohar Nussinov
    • , Alexander Seidel
    • , Shaw-Wei D. Tsen
    •  & Samuel Achilefu
  • Author Correction |

    • David B. Chou
    • , Viktoras Frismantas
    • , Yuka Milton
    • , Rhiannon David
    • , Petar Pop-Damkov
    • , Douglas Ferguson
    • , Alexander MacDonald
    • , Özge Vargel Bölükbaşı
    • , Cailin E. Joyce
    • , Liliana S. Moreira Teixeira
    • , Arianna Rech
    • , Amanda Jiang
    • , Elizabeth Calamari
    • , Sasan Jalili-Firoozinezhad
    • , Brooke A. Furlong
    • , Lucy R. O’Sullivan
    • , Carlos F. Ng
    • , Youngjae Choe
    • , Susan Marquez
    • , Kasiani C. Myers
    • , Olga K. Weinberg
    • , Robert P. Hasserjian
    • , Richard Novak
    • , Oren Levy
    • , Rachelle Prantil-Baun
    • , Carl D. Novina
    • , Akiko Shimamura
    • , Lorna Ewart
    •  & Donald E. Ingber
  • Article |

    A reconfigurable nanophotonic silicon probe, implanted in anaesthetized mice, that switches multiple optical beams in less than 20 μs enables the optical stimulation of multineuron spike patterns in the brain at sub-millisecond precision.

    • Aseema Mohanty
    • , Qian Li
    • , Mohammad Amin Tadayon
    • , Samantha P. Roberts
    • , Gaurang R. Bhatt
    • , Euijae Shim
    • , Xingchen Ji
    • , Jaime Cardenas
    • , Steven A. Miller
    • , Adam Kepecs
    •  & Michal Lipson
  • Article |

    A single removable transdermal patch bearing microneedles loaded with insulin and a non-degradable glucose-responsive polymeric matrix regulates blood glucose in insulin-deficient diabetic mice and minipigs.

    • Jicheng Yu
    • , Jinqiang Wang
    • , Yuqi Zhang
    • , Guojun Chen
    • , Weiwei Mao
    • , Yanqi Ye
    • , Anna R. Kahkoska
    • , John B. Buse
    • , Robert Langer
    •  & Zhen Gu
  • Article |

    FeCo nanoparticles with a graphitic carbon shell decorated with poly(ethylene glycol) have photothermal and magnetothermal properties and are sensitive tracers for magnetic particle imaging, magnetic resonance imaging and photoacoustic imaging.

    • Guosheng Song
    • , Michael Kenney
    • , Yun-Sheng Chen
    • , Xianchuang Zheng
    • , Yong Deng
    • , Zhuo Chen
    • , Shan X. Wang
    • , Sanjiv Sam Gambhir
    • , Hongjie Dai
    •  & Jianghong Rao
  • Article |

    A vascularized human bone-marrow-on-a-chip improves the maintenance of patient-derived CD34+ cells, and recapitulates clinically relevant aspects of bone marrow injury as well as key haematopoietic defects of patients with a rare genetic disorder.

    • David B. Chou
    • , Viktoras Frismantas
    • , Yuka Milton
    • , Rhiannon David
    • , Petar Pop-Damkov
    • , Douglas Ferguson
    • , Alexander MacDonald
    • , Özge Vargel Bölükbaşı
    • , Cailin E. Joyce
    • , Liliana S. Moreira Teixeira
    • , Arianna Rech
    • , Amanda Jiang
    • , Elizabeth Calamari
    • , Sasan Jalili-Firoozinezhad
    • , Brooke A. Furlong
    • , Lucy R. O’Sullivan
    • , Carlos F. Ng
    • , Youngjae Choe
    • , Susan Marquez
    • , Kasiani C. Myers
    • , Olga K. Weinberg
    • , Robert P. Hasserjian
    • , Richard Novak
    • , Oren Levy
    • , Rachelle Prantil-Baun
    • , Carl D. Novina
    • , Akiko Shimamura
    • , Lorna Ewart
    •  & Donald E. Ingber
  • Article |

    A method that quantifies signal reductions in proton magnetic resonance spectroscopy resulting from the replacement of 1H with 2H after the administration of a deuterated substrate can be used to monitor the turnover of cellular metabolites in vivo.

    • Laurie J. Rich
    • , Puneet Bagga
    • , Neil E. Wilson
    • , Mitchell D. Schnall
    • , John A. Detre
    • , Mohammad Haris
    •  & Ravinder Reddy
  • Article |

    Physiological modelling of first-pass metabolism using data from a robotic instrument that fluidically links relevant organ chips predicts human pharmacokinetic parameters for orally administered nicotine and for intravenously injected cisplatin.

    • Anna Herland
    • , Ben M. Maoz
    • , Debarun Das
    • , Mahadevabharath R. Somayaji
    • , Rachelle Prantil-Baun
    • , Richard Novak
    • , Michael Cronce
    • , Tessa Huffstater
    • , Sauveur S. F. Jeanty
    • , Miles Ingram
    • , Angeliki Chalkiadaki
    • , David Benson Chou
    • , Susan Marquez
    • , Aaron Delahanty
    • , Sasan Jalili-Firoozinezhad
    • , Yuka Milton
    • , Alexandra Sontheimer-Phelps
    • , Ben Swenor
    • , Oren Levy
    • , Kevin K. Parker
    • , Andrzej Przekwas
    •  & Donald E. Ingber
  • Article |

    A system employing liquid-handling robotics and an integrated mobile microscope enables the automated culture, sample collection and in situ microscopy imaging of up to ten fluidically coupled organ chips within a standard tissue-culture incubator.

    • Richard Novak
    • , Miles Ingram
    • , Susan Marquez
    • , Debarun Das
    • , Aaron Delahanty
    • , Anna Herland
    • , Ben M. Maoz
    • , Sauveur S. F. Jeanty
    • , Mahadevabharath R. Somayaji
    • , Morgan Burt
    • , Elizabeth Calamari
    • , Angeliki Chalkiadaki
    • , Alexander Cho
    • , Youngjae Choe
    • , David Benson Chou
    • , Michael Cronce
    • , Stephanie Dauth
    • , Toni Divic
    • , Jose Fernandez-Alcon
    • , Thomas Ferrante
    • , John Ferrier
    • , Edward A. FitzGerald
    • , Rachel Fleming
    • , Sasan Jalili-Firoozinezhad
    • , Thomas Grevesse
    • , Josue A. Goss
    • , Tiama Hamkins-Indik
    • , Olivier Henry
    • , Chris Hinojosa
    • , Tessa Huffstater
    • , Kyung-Jin Jang
    • , Ville Kujala
    • , Lian Leng
    • , Robert Mannix
    • , Yuka Milton
    • , Janna Nawroth
    • , Bret A. Nestor
    • , Carlos F. Ng
    • , Blakely O’Connor
    • , Tae-Eun Park
    • , Henry Sanchez
    • , Josiah Sliz
    • , Alexandra Sontheimer-Phelps
    • , Ben Swenor
    • , Guy Thompson II
    • , George J. Touloumes
    • , Zachary Tranchemontagne
    • , Norman Wen
    • , Moran Yadid
    • , Anthony Bahinski
    • , Geraldine A. Hamilton
    • , Daniel Levner
    • , Oren Levy
    • , Andrzej Przekwas
    • , Rachelle Prantil-Baun
    • , Kevin K. Parker
    •  & Donald E. Ingber
  • Article |

    Optimized adeno-associated viruses delivering split cytosine base editors and adenine base editors with trans-splicing inteins can edit brain, liver, retina, heart and skeletal-muscle tissues at therapeutically relevant efficiencies.

    • Jonathan M. Levy
    • , Wei-Hsi Yeh
    • , Nachiket Pendse
    • , Jessie R. Davis
    • , Erin Hennessey
    • , Rossano Butcher
    • , Luke W. Koblan
    • , Jason Comander
    • , Qin Liu
    •  & David R. Liu