Articles

The controlled in situ formation of biomolecular condensates as intracellular reservoirs for the enrichment and retention of a chemotherapeutic in cancer cells enhanced antitumour activities in mice with drug-resistant tumours.

A commercial near-infrared dye that is sensitive to apoptosis and that provides high tumour-to-muscle and tumour-to-liver contrast ratios facilitates fluorescence-guided tumour screening, tumour resection and the assessment of wound healing.

The viral delivery of a miniaturized form of a master protein that establishes dyads (nanostructures involved in excitation–contraction coupling in cardiomyocytes) improved dyad architecture and normalized cardiac function under pressure overload.

A light-sheet fluorescence microscope leveraging consumer-grade components as well as optimized optics and software facilitates the high-resolution imaging of cleared and living samples at scale with lower costs.

A latent diffusion model pre-trained on pairs of natural images and text descriptors can be adapted to generate diverse and visually plausible synthetic chest X-ray images whose appearance can be controlled with free-form medical text prompts.

Two simple and efficient methods for RNA circularization and a complementary Cas9 deimmunization method enhance a broad range of applications in genome and cell engineering.

The hearing-loss phenotype can be corrected via the injection, into the inner ear, of a base editor packaged in an adeno-associated virus, as shown in mice harbouring a mutation homozygous to human OTOF and in humanized mice with the human mutation.

An analysis of neuroimaging data across mental health, neurodegenerative, psychiatric and neurodevelopmental diagnostic categories highlights how brain network organization and chemoarchitecture interact to shape cognitive topographies.

A high-throughput mutation-scanning method for interrogating sequence variation in antimicrobial peptides facilitates the understanding of sequence–structure–function relationships affecting the membrane selectivity of antimicrobial peptides.

The generation and maintenance of peri-implant fibrotic tissue requires skeletal cells expressing the leptin receptor and can be prevented and reversed via the functional inhibition of these cells.

Human B cells can be edited to produce custom heavy-chain antibodies while allowing the cells to respond to immunization with a matched antigen.

Platinum-based chemotherapeutics can be locally released in tumours by leveraging the radiation-induced reduction of platinum prodrugs.

The efficacy of a B-cell-lymphoma-2-homology-3 mimetic can be predicted by profiling the rewired status of the network of interactions among proteins of the B-cell-lymphoma-2 family via single-molecule pull-down and co-immunoprecipitation.

A systematic implementation of six efficiency optimizations for prime editing led to high levels of functional correction, in airway epithelial cells, of the predominant mutation that causes cystic fibrosis.

The clinical detection of fluorescent protoporphyrin IX during glioma-resection surgery can be improved via a microscope that pairs stimulated Raman histology and two-photon excitation fluorescence microscopy.

Desired cell-state transitions can be guided via the activation of gene expression through a constitutively expressed endoribonuclease whose translation is regulated by endogenous cell-state-specific miRNAs.

An optimized head-mounted fluorescent mesoscope enables large-scale calcium imaging at single-neuron resolution in freely moving mice, facilitating neurobehavioural studies during social interactions and fear-conditioning experiments.

A method leveraging antibodies conjugated with barcoded DNA strands to linearize closely spaced interactions from individual 3D protein complexes allows for the mapping of pairwise and higher-order protein interactions within cells.

A digital pathology–artificial intelligence framework that leverages active learning and clinician-in-the-loop real-time feedback improves performance in diagnostic tasks.

Recreation of the truncated form of the erythropoietin receptor in human haematopoietic stem and progenitor cells via Cas9-mediated genome editing gives the edited cells and their erythropoietic progeny a proliferative advantage.