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An assay leveraging the target-specific splinted ligation of DNA probes to generate expression cassettes for the cell-free synthesis of reporter proteins accurately detects nucleic acids at ambient temperature.
A high-throughput method leveraging peptide-encoding mRNA barcodes for the in vivo screening of libraries of nanoparticle formulations allows for the rapid optimization of a lipid nanoparticle for the delivery of mRNA to the liver.
Ensembles of explainable machine-learning models increase the quality of explanations for the molecular basis of synergetic drug combinations, as shown for the treatment of acute myeloid leukaemia.
An anti-inflammatory enzyme fused with a tissue-anchoring protein and injected into inflamed tissues ameliorates local inflammation without causing systemic immune suppression, as shown in multiple rodent models of inflammatory diseases.
A method leveraging an integrase-deficient lentivirus, homology-directed repair and the electroporation of a CRISPR-associated ribonucleoprotein complex allows for the knock-in and stable expression of large payloads in primary human cells.
The sensitivity, chemical specificity and spatiotemporal resolution of proton magnetic resonance spectroscopic imaging at 7 T allow for the discrimination of deuterated and non-deuterated neurotransmitters and glucose metabolites in the human brain.
A rapid one-pot isothermal assay that leverages rolling-circle amplification and the endonuclease Cas12a can accurately detect specific miRNAs in extracellular vesicles in patient plasma.
A tether-less and battery-less implant allows for the recording of electroencephalograms, electromyograms and body temperature in freely moving small animals, and for closed-loop neuromodulation via optogenetics and pharmacology.
The throughput of the in vivo screening of hydrogels for antifibrotic properties can be increased by tagging the biomaterials with cells and reading their genotype via next-generation sequencing.
The yields of edited primary human lymphocytes can be increased substantially, with respect to those obtained via electroporation, by delivering a CRISPR ribonucleoprotein alongside an amphiphilic peptide identified via screening.
Durable anti-tumour responses can be triggered by maximizing the cooperative phagocytic potency of macrophages through the disruption of the CD47–SIRPα macrophage checkpoint and by delivering a tumour-opsonizing monoclonal antibody.
Prime editing can efficiently correct the sickle-cell allele to produce wild-type haemoglobin in patient haematopoietic stem cells that engraft efficiently in mice, yielding erythrocytes resistant to hypoxia-induced sickling.
A lentiviral vector incorporating two functionally independent promoters allows for the generation of T cells that express a tumour-directed receptor constitutively and an inducible gene that is expressed only in the presence of a target antigen.
A swallowable X-ray dosimeter detects the absolute absorbed radiation dose alongside changes in pH and temperature in the gastrointestinal tract of rabbits.
The activity of standard Cas9-based genome-editing systems can be constrained by the addition of cytosine stretches to the 5′-end of conventional single-guide RNAs.
The crosslinking of extracellular matrix in cirrhotic liver tissue is mediated by advanced glycation end-products and can be inhibited by rosmarinic acid.
A battery-less and wireless integrated sensor implanted in the heart or in a blood vessel can measure blood pressure, flow rate and temperature simultaneously and in real time, as shown in large animal models.
Potent circulating tumour-reactive lymphocytes can be isolated from peripheral blood at high yield and purity via microfluidic immunomagnetic cell sorting.
A method for the sequential assessment of the physical phenotypes of suspended cells singularized from tissue biopsies by mechanical dissociation allows for rapid diagnoses.
