Browse Articles

  • Article |

    Antibody and antibody-avidity assays relying on near-infrared-fluorescence amplification by nanostructured plasmonic gold substrates accurately quantify antibodies to SARS-CoV-2 and to common viruses in human serum and saliva.

    • Tiancheng Liu
    • , Jessica Hsiung
    • , Su Zhao
    • , Jessica Kost
    • , Deepika Sreedhar
    • , Carl V. Hanson
    • , Kjerstie Olson
    • , Douglas Keare
    • , Shin Ting Chang
    • , Kevin P. Bliden
    • , Paul A. Gurbel
    • , Udaya S. Tantry
    • , John Roche
    • , Cynthia Press
    • , John Boggs
    • , Jorge P. Rodriguez-Soto
    • , Jose G. Montoya
    • , Meijie Tang
    •  & Hongjie Dai
  • Article |

    Chiral photons can accelerate the differentiation of neural stem cells into neurons in vitro and in vivo when DNA-bridged chiral assemblies of gold nanoparticles are tightly entangled with the cells’ cytoskeletal fibres.

    • Aihua Qu
    • , Maozhong Sun
    • , Ji-Young Kim
    • , Liguang Xu
    • , Changlong Hao
    • , Wei Ma
    • , Xiaoling Wu
    • , Xiaogang Liu
    • , Hua Kuang
    • , Nicholas A. Kotov
    •  & Chuanlai Xu
  • News & Views |

    Wearable piezoelectric thin films integrated with multiphysics modelling and three-dimensional digital image correlation can decode facial expressions in real time.

    • Xiaoxiang Gao
    • , Xiangjun Chen
    •  & Sheng Xu
  • News & Views |

    Narrow-bandwidth signals and relaxed neural-recording parameters can substantially reduce the power requirements of brain–machine interfaces without degrading their performance.

    • Marc W. Slutzky
  • Article |

    An integrated system consisting of conformable piezoelectric devices, three-dimensional digital image correlation, multiphysics modelling and real-time classification algorithms predicts facial kinematics and decodes facial deformations.

    • Tao Sun
    • , Farita Tasnim
    • , Rachel T. McIntosh
    • , Nikta Amiri
    • , Dana Solav
    • , Mostafa Tavakkoli Anbarani
    • , David Sadat
    • , Lin Zhang
    • , Yuandong Gu
    • , M. Amin Karami
    •  & Canan Dagdeviren
  • Article |

    Two clinically relevant subtypes of post-traumatic stress disorder and major depressive disorder have been identified via machine learning analyses of functional connectivity patterns in resting-state electroencephalography.

    • Yu Zhang
    • , Wei Wu
    • , Russell T. Toll
    • , Sharon Naparstek
    • , Adi Maron-Katz
    • , Mallissa Watts
    • , Joseph Gordon
    • , Jisoo Jeong
    • , Laura Astolfi
    • , Emmanuel Shpigel
    • , Parker Longwell
    • , Kamron Sarhadi
    • , Dawlat El-Said
    • , Yuanqing Li
    • , Crystal Cooper
    • , Cherise Chin-Fatt
    • , Martijn Arns
    • , Madeleine S. Goodkind
    • , Madhukar H. Trivedi
    • , Charles R. Marmar
    •  & Amit Etkin
  • Editorial |

    In less than a decade, the genome-editing technology now recognized by the Nobel Prize in Chemistry has impacted the biological and biomedical sciences widely. What’s next for CRISPR in biomedicine?

  • Article |

    Deep-learning models for the automated measurement of retinal-vessel calibre in retinal photographs perform comparably to or better than expert graders in associations of measurements of retinal-vessel calibre with cardiovascular risk factors.

    • Carol Y. Cheung
    • , Dejiang Xu
    • , Ching-Yu Cheng
    • , Charumathi Sabanayagam
    • , Yih-Chung Tham
    • , Marco Yu
    • , Tyler Hyungtaek Rim
    • , Chew Yian Chai
    • , Bamini Gopinath
    • , Paul Mitchell
    • , Richie Poulton
    • , Terrie E. Moffitt
    • , Avshalom Caspi
    • , Jason C. Yam
    • , Clement C. Tham
    • , Jost B. Jonas
    • , Ya Xing Wang
    • , Su Jeong Song
    • , Louise M. Burrell
    • , Omar Farouque
    • , Ling Jun Li
    • , Gavin Tan
    • , Daniel S. W. Ting
    • , Wynne Hsu
    • , Mong Li Lee
    •  & Tien Y. Wong
  • Article |

    The sustained release of a leukaemia-associated epitope peptide and of anti-PD-1 antibody co-encapsulated in degradable microcapsules results in superior therapeutic outcomes in mouse models of leukaemia.

    • Xiaoling Xie
    • , Yuxing Hu
    • , Tong Ye
    • , Yiran Chen
    • , Lijuan Zhou
    • , Feng Li
    • , Xiaobo Xi
    • , Shuang Wang
    • , Yanjie He
    • , Xiaoyong Gao
    • , Wei Wei
    • , Guanghui Ma
    •  & Yuhua Li
  • Article |

    The enhanced accumulation and residence time of systemically administered interleukin-4 fused to serum albumin in lymph nodes and in the spleen prevents the development of multiple sclerosis in mice.

    • Ako Ishihara
    • , Jun Ishihara
    • , Elyse A. Watkins
    • , Andrew C. Tremain
    • , Mindy Nguyen
    • , Ani Solanki
    • , Kiyomitsu Katsumata
    • , Aslan Mansurov
    • , Erica Budina
    • , Aaron T. Alpar
    • , Peyman Hosseinchi
    • , Lea Maillat
    • , Joseph W. Reda
    • , Takahiro Kageyama
    • , Melody A. Swartz
    • , Eiji Yuba
    •  & Jeffrey A. Hubbell
  • Article |

    Systemically injected lipid–polymer nanoparticles encapsulating small interfering RNA for silencing genes in bone-marrow endothelial cells of mice improved the healing of the mice after myocardial infarction.

    • Marvin Krohn-Grimberghe
    • , Michael J. Mitchell
    • , Maximilian J. Schloss
    • , Omar F. Khan
    • , Gabriel Courties
    • , Pedro P. G. Guimaraes
    • , David Rohde
    • , Sebastian Cremer
    • , Piotr S. Kowalski
    • , Yuan Sun
    • , Mingchee Tan
    • , Jamie Webster
    • , Karin Wang
    • , Yoshiko Iwamoto
    • , Stephen P. Schmidt
    • , Gregory R. Wojtkiewicz
    • , Ribhu Nayar
    • , Vanessa Frodermann
    • , Maarten Hulsmans
    • , Amanda Chung
    • , Friedrich Felix Hoyer
    • , Filip K. Swirski
    • , Robert Langer
    • , Daniel G. Anderson
    •  & Matthias Nahrendorf
  • Article
    | Open Access

    An open-source integrated knee–ankle robotic prosthesis facilitates the testing of control strategies and biomechanics in real-world environments.

    • Alejandro F. Azocar
    • , Luke M. Mooney
    • , Jean-François Duval
    • , Ann M. Simon
    • , Levi J. Hargrove
    •  & Elliott J. Rouse
  • Article |

    A web tool enables the rapid design of prime-editing guide RNAs from reference and edited DNA sequences, for diverse prime-editing applications.

    • Ryan D. Chow
    • , Jennifer S. Chen
    • , Johanna Shen
    •  & Sidi Chen
  • Article |

    Optical contrast agents using AND-gate logic enhance the specificity and sensitivity of fluorescence-guided imaging in the resection of tumours and in the detection of metastases in mouse models of cancer.

    • John C. Widen
    • , Martina Tholen
    • , Joshua J. Yim
    • , Alexander Antaris
    • , Kerriann M. Casey
    • , Stephan Rogalla
    • , Alwin Klaassen
    • , Jonathan Sorger
    •  & Matthew Bogyo
  • Article |

    A microfluidic assay that quantifies cell migration and proliferation can prospectively categorize patients with glioblastoma according to progression-free survival.

    • Bin Sheng Wong
    • , Sagar R. Shah
    • , Christopher L. Yankaskas
    • , Vivek K. Bajpai
    • , Pei-Hsun Wu
    • , Deborah Chin
    • , Brent Ifemembi
    • , Karim ReFaey
    • , Paula Schiapparelli
    • , Xiaobin Zheng
    • , Stuart S. Martin
    • , Chen-Ming Fan
    • , Alfredo Quiñones-Hinojosa
    •  & Konstantinos Konstantopoulos
  • Article |

    An intravenously administered electric-field-sensitive contrast agent for magnetic resonance imaging that crosses the blood–brain barrier improves lesion visualization with high sensitivity and target-to-background ratio in mice.

    • Cong Wang
    • , Wanbing Sun
    • , Jun Zhang
    • , Jianping Zhang
    • , Qinghua Guo
    • , Xingyu Zhou
    • , Dandan Fan
    • , Haoran Liu
    • , Ming Qi
    • , Xihui Gao
    • , Haiyan Xu
    • , Zhaobing Gao
    • , Mei Tian
    • , Hong Zhang
    • , Jianhong Wang
    • , Zixuan Wei
    • , Nicholas J. Long
    • , Ying Mao
    •  & Cong Li
  • Article |

    Pigs can be genetically modified to inactivate endogenous retroviruses and to display enhanced compatibility with the human immune system using a combination of CRISPR–Cas9 and transposon technologies.

    • Yanan Yue
    • , Weihong Xu
    • , Yinan Kan
    • , Hong-Ye Zhao
    • , Yixuan Zhou
    • , Xiaobin Song
    • , Jiajia Wu
    • , Juan Xiong
    • , Dharmendra Goswami
    • , Meng Yang
    • , Lydia Lamriben
    • , Mengyuan Xu
    • , Qi Zhang
    • , Yu Luo
    • , Jianxiong Guo
    • , Shengyi Mao
    • , Deling Jiao
    • , Tien Dat Nguyen
    • , Zhuo Li
    • , Jacob V. Layer
    • , Mailin Li
    • , Violette Paragas
    • , Michele E. Youd
    • , Zhongquan Sun
    • , Yuan Ding
    • , Weilin Wang
    • , Hongwei Dou
    • , Lingling Song
    • , Xueqiong Wang
    • , Lei Le
    • , Xin Fang
    • , Haydy George
    • , Ranjith Anand
    • , Shi Yun Wang
    • , William F. Westlin
    • , Marc Güell
    • , James Markmann
    • , Wenning Qin
    • , Yangbin Gao
    • , Hong-Jiang Wei
    • , George M. Church
    •  & Luhan Yang
  • Article |

    Customized soft electrode arrays that are well adjusted to specific anatomical environments, functions and experimental models can be rapidly prototyped via the robotically controlled deposition of conductive inks and insulating inks.

    • Dzmitry Afanasenkau
    • , Daria Kalinina
    • , Vsevolod Lyakhovetskii
    • , Christoph Tondera
    • , Oleg Gorsky
    • , Seyyed Moosavi
    • , Natalia Pavlova
    • , Natalia Merkulyeva
    • , Allan V. Kalueff
    • , Ivan R. Minev
    •  & Pavel Musienko
  • Article |

    A multiplexed fluorescence-based assay detects seroconversion in individuals infected with SARS-CoV-2 from less than 1 µl of blood as early as the day of the first positive nasopharyngeal nucleic acid test after symptom onset.

    • Maia Norman
    • , Tal Gilboa
    • , Alana F. Ogata
    • , Adam M. Maley
    • , Limor Cohen
    • , Evan L. Busch
    • , Roey Lazarovits
    • , Chih-Ping Mao
    • , Yongfei Cai
    • , Jun Zhang
    • , Jared E. Feldman
    • , Blake M. Hauser
    • , Timothy M. Caradonna
    • , Bing Chen
    • , Aaron G. Schmidt
    • , Galit Alter
    • , Richelle C. Charles
    • , Edward T. Ryan
    •  & David R. Walt
  • Article |

    Molecular and physiological phenotypes in mouse models of myotonic dystrophy type I can be sustainably reversed by CRISPR-mediated targeting of toxic RNA repeats.

    • Ranjan Batra
    • , David A. Nelles
    • , Daniela M. Roth
    • , Florian Krach
    • , Curtis A. Nutter
    • , Takahiro Tadokoro
    • , James D. Thomas
    • , Łukasz J. Sznajder
    • , Steven M. Blue
    • , Haydee L. Gutierrez
    • , Patrick Liu
    • , Stefan Aigner
    • , Oleksandr Platoshyn
    • , Atsushi Miyanohara
    • , Martin Marsala
    • , Maurice S. Swanson
    •  & Gene W. Yeo
  • Editorial |

    Obtaining valuable datasets is often arduous, costly and time-consuming. Give them away for wider reuse.

  • News & Views |

    Biomaterial structures can be printed with near-infrared light within the tissues of living animals by using photosensitive polymer hydrogels.

    • Mark W. Tibbitt
  • News & Views |

    Microwell arrays enable the culture of thousands of organoids with increased homogeneity, and facilitate high-content image-based analyses and high-throughput drug screens.

    • R. Jarrett Bliton
    •  & Scott T. Magness
  • News & Views |

    The oral delivery of a microencapsulated bacterial cocktail into animal models of kidney disease promotes the degradation of nitrogenous waste in the gut, thereby supporting renal function.

    • John Jarman
    •  & Dylan Dodd
  • Article |

    A gelling hypotonic solution for ocular delivery that forms a uniform and clear thin layer conformal to the ocular surface resists clearance from blinking and extends the residence time of the delivered drugs.

    • Yoo Chun Kim
    • , Matthew D. Shin
    • , Sean F. Hackett
    • , Henry T. Hsueh
    • , Raquel Lima e Silva
    • , Abhijit Date
    • , Hyounkoo Han
    • , Byung-Jin Kim
    • , Amy Xiao
    • , Youngwook Kim
    • , Laolu Ogunnaike
    • , Nicole M. Anders
    • , Avelina Hemingway
    • , Ping He
    • , Albert S. Jun
    • , Peter J. McDonnell
    • , Charles Eberhart
    • , Ian Pitha
    • , Donald J. Zack
    • , Peter A. Campochiaro
    • , Justin Hanes
    •  & Laura M. Ensign
  • Article |

    Soft multilayer electronic arrays on endocardial balloon catheters allow for multiplexed high-density spatiotemporal sensing and actuation, as shown in perfused ex vivo hearts.

    • Mengdi Han
    • , Lin Chen
    • , Kedar Aras
    • , Cunman Liang
    • , Xuexian Chen
    • , Hangbo Zhao
    • , Kan Li
    • , Ndeye Rokhaya Faye
    • , Bohan Sun
    • , Jae-Hwan Kim
    • , Wubin Bai
    • , Quansan Yang
    • , Yuhang Ma
    • , Wei Lu
    • , Enming Song
    • , Janice Mihyun Baek
    • , Yujin Lee
    • , Clifford Liu
    • , Jeffrey B. Model
    • , Guanjun Yang
    • , Roozbeh Ghaffari
    • , Yonggang Huang
    • , Igor R. Efimov
    •  & John A. Rogers
  • Article |

    The specific high-sensitivity enzymatic reporter unlocking (SHERLOCK) assay detected severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA with high sensitivity and specificity in hundreds of nasopharyngeal and throat swab samples collected at Siriraj Hospital in Thailand.

    • Maturada Patchsung
    • , Krittapas Jantarug
    • , Archiraya Pattama
    • , Kanokpol Aphicho
    • , Surased Suraritdechachai
    • , Piyachat Meesawat
    • , Khomkrit Sappakhaw
    • , Nattawat Leelahakorn
    • , Theerawat Ruenkam
    • , Thanakrit Wongsatit
    • , Niracha Athipanyasilp
    • , Bhumrapee Eiamthong
    • , Benya Lakkanasirorat
    • , Thitima Phoodokmai
    • , Nootaree Niljianskul
    • , Danaya Pakotiprapha
    • , Sittinan Chanarat
    • , Aimorn Homchan
    • , Ruchanok Tinikul
    • , Philaiwarong Kamutira
    • , Kochakorn Phiwkaow
    • , Sahachat Soithongcharoen
    • , Chadaporn Kantiwiriyawanitch
    • , Vinutsada Pongsupasa
    • , Duangthip Trisrivirat
    • , Juthamas Jaroensuk
    • , Thanyaporn Wongnate
    • , Somchart Maenpuen
    • , Pimchai Chaiyen
    • , Sirichai Kamnerdnakta
    • , Jirawat Swangsri
    • , Suebwong Chuthapisith
    • , Yongyut Sirivatanauksorn
    • , Chutikarn Chaimayo
    • , Ruengpung Sutthent
    • , Wannee Kantakamalakul
    • , Julia Joung
    • , Alim Ladha
    • , Xin Jin
    • , Jonathan S. Gootenberg
    • , Omar O. Abudayyeh
    • , Feng Zhang
    • , Navin Horthongkham
    •  & Chayasith Uttamapinant
  • News & Views |

    Positron emission tomography enables the tracking, in a mouse, of an intravenously injected cell radiolabelled with mesoporous silica nanoparticles containing 68Ga.

    • Guus A. M. S. van Dongen
    • , Ronald Boellaard
    •  & Danielle J. Vugts
  • News & Views |

    Lipofuscin, an endogenous pigment, can be used as a near-infrared fluorescent biomarker to stage and to monitor the progression of chronic liver disease, as shown in mice with induced liver disease and in human livers biopsied from patients with liver fibrosis.

    • Justin J. O’Sullivan
    • , Nathaniel H. O. Harder
    •  & Marie C. Heffern
  • Editorial |

    Results from the early trials of vaccine candidates against SARS-CoV-2 signal the fitness of vaccine technologies soon to be tested at scale.

  • News & Views |

    The cell-selective permeability of a retrievable microporous device encapsulating human therapeutic cells and implanted in the intraperitoneal space of mice provides long-term protection to the transplanted cells by dampening foreign-body responses and preventing the immune rejection of the graft.

    • Ken Coppieters
    • , Louise Winkel
    •  & Matthias von Herrath
  • Article |

    Intratumoural injection of a self-assembling supramolecular hydrogel that slowly releases a STING agonist and a chemotherapeutic agent reduces tumour immunosuppression and increases antitumour responses in mice.

    • Feihu Wang
    • , Hao Su
    • , Dongqing Xu
    • , Wenbing Dai
    • , Weijie Zhang
    • , Zongyuan Wang
    • , Caleb F. Anderson
    • , Mengzhen Zheng
    • , Richard Oh
    • , Fengyi Wan
    •  & Honggang Cui
  • News & Views |

    Neuropathologies can be classified, on the basis of post-mortem histopathology and by using machine learning, into six transdiagnostic clusters associated with clinical phenotypes.

    • Pablo Villoslada
    • , Ricardo Baeza-Yates
    •  & Joseph C. Masdeu
  • Article |

    Analyses of data from intracortical brain–computer interfaces implanted in rhesus macaques and in a human suggest that the circuit design parameters of current recording interfaces can be relaxed considerably without loss of performance.

    • Nir Even-Chen
    • , Dante G. Muratore
    • , Sergey D. Stavisky
    • , Leigh R. Hochberg
    • , Jaimie M. Henderson
    • , Boris Murmann
    •  & Krishna V. Shenoy
  • Article |

    Post-mortem histopathological data can be used to classify neuropathologies into six transdiagnostic clusters, and patient membership to these clusters can be predicted from cognitive scores, genotype and protein levels in cerebrospinal fluid.

    • Eli J. Cornblath
    • , John L. Robinson
    • , David J. Irwin
    • , Edward B. Lee
    • , Virginia M.-Y. Lee
    • , John Q. Trojanowski
    •  & Danielle S. Bassett
  • Article |

    An antibody-discovery pipeline integrating single-cell mRNA-sequence analysis, bioinformatics, synthetic biology and high-throughput functional analyses enables the rapid discovery of potent human monoclonal antibodies against viral pathogens.

    • Pavlo Gilchuk
    • , Robin G. Bombardi
    • , Jesse H. Erasmus
    • , Qing Tan
    • , Rachel Nargi
    • , Cinque Soto
    • , Peter Abbink
    • , Todd J. Suscovich
    • , Lorellin A. Durnell
    • , Amit Khandhar
    • , Jacob Archer
    • , Jenny Liang
    • , Mallorie E. Fouch
    • , Edgar Davidson
    • , Benjamin J. Doranz
    • , Taylor Jones
    • , Elise Larson
    • , Stacey Ertel
    • , Brian Granger
    • , Jasmine Fuerte-Stone
    • , Vicky Roy
    • , Thomas Broge
    • , Thomas C. Linnekin
    • , Caitlyn H. Linde
    • , Matthew J. Gorman
    • , Joseph Nkolola
    • , Galit Alter
    • , Steven G. Reed
    • , Dan H. Barouch
    • , Michael S. Diamond
    • , James E. Crowe Jr
    • , Neal Van Hoeven
    • , Larissa B. Thackray
    •  & Robert H. Carnahan
  • Article |

    T cells can be classified according to their activation state and subtype on the basis of their autofluorescence lifetime patterns.

    • Alex J. Walsh
    • , Katherine P. Mueller
    • , Kelsey Tweed
    • , Isabel Jones
    • , Christine M. Walsh
    • , Nicole J. Piscopo
    • , Natalie M. Niemi
    • , David J. Pagliarini
    • , Krishanu Saha
    •  & Melissa C. Skala
  • Article |

    The 300–1,000 Hz band of spiking activity, dominated by local single-unit spikes, can enhance the decoding performance of neural interfaces.

    • Samuel R. Nason
    • , Alex K. Vaskov
    • , Matthew S. Willsey
    • , Elissa J. Welle
    • , Hyochan An
    • , Philip P. Vu
    • , Autumn J. Bullard
    • , Chrono S. Nu
    • , Jonathan C. Kao
    • , Krishna V. Shenoy
    • , Taekwang Jang
    • , Hun-Seok Kim
    • , David Blaauw
    • , Parag G. Patil
    •  & Cynthia A. Chestek
  • Article |

    The expression of therapeutic protein targets in circulating tumour cells isolated from blood samples of patients with cancer can be tracked at the single-cell level by antibody-mediated magnetic labelling and microfluidic sorting.

    • Mahmoud Labib
    • , Zongjie Wang
    • , Sharif U. Ahmed
    • , Reza M. Mohamadi
    • , Bill Duong
    • , Brenda Green
    • , Edward H. Sargent
    •  & Shana O. Kelley