Browse Articles

  • Article |

    A method that combines a depth-sensing camera and machine learning can track the movements of up to four mice in real time and for several days, extracting both individual and group behavioural traits.

    • Fabrice de Chaumont
    • , Elodie Ey
    • , Nicolas Torquet
    • , Thibault Lagache
    • , Stéphane Dallongeville
    • , Albane Imbert
    • , Thierry Legou
    • , Anne-Marie Le Sourd
    • , Philippe Faure
    • , Thomas Bourgeron
    •  & Jean-Christophe Olivo-Marin
  • Comment |

    The development of implantable electrode arrays that broadly and seamlessly integrate with brain tissue will require innovation that responsibly considers clinically relevant neuroethical concerns.

    • Laura Cabrera
    • , Charles Sadle
    •  & Erin Purcell
  • Article |

    A microfluidic intestine-on-a-chip that allows the control of physiologically relevant oxygen gradients, enables the extended coculture of living human intestinal epithelium with stable communities of aerobic and anaerobic human gut microbiota.

    • Sasan Jalili-Firoozinezhad
    • , Francesca S. Gazzaniga
    • , Elizabeth L. Calamari
    • , Diogo M. Camacho
    • , Cicely W. Fadel
    • , Amir Bein
    • , Ben Swenor
    • , Bret Nestor
    • , Michael J. Cronce
    • , Alessio Tovaglieri
    • , Oren Levy
    • , Katherine E. Gregory
    • , David T. Breault
    • , Joaquim M. S. Cabral
    • , Dennis L. Kasper
    • , Richard Novak
    •  & Donald E. Ingber
  • News & Views |

    The oxygen consumption rates of single tumour cells can be measured via photoacoustic microscopy, by leveraging haemoglobin as both an oxygen supplier and an oxygen sensor.

    • Alex J. Walsh
    • , Joe T. Sharick
    •  & Melissa C. Skala
  • News & Views |

    The biodistribution of the components of a messenger RNA vaccine following its administration in non-human primates can be non-invasively monitored by labelling the vaccine with a dual radionuclide–near-infrared probe.

    • Sebastian Ols
    •  & Karin Loré
  • Editorial |

    The development of imaging technology for the quantification of optical biomarkers of pathological processes should involve the validation of the biomarkers’ biological accuracy.

  • News & Views |

    High-speed optoacoustic tomography can monitor the neural activity of a whole mouse brain, by using a genetically encoded calcium sensor originally developed for fluorescence microscopy.

    • Alessio Andreoni
    •  & Lin Tian
  • Article |

    A microfluidic assay predicts the metastatic potential of breast cancer specimens by quantifying the abundance and proliferative index of the migratory cells within them.

    • Christopher L. Yankaskas
    • , Keyata N. Thompson
    • , Colin D. Paul
    • , Michele I. Vitolo
    • , Panagiotis Mistriotis
    • , Ankit Mahendra
    • , Vivek K. Bajpai
    • , Daniel J. Shea
    • , Kristen M. Manto
    • , Andreas C. Chai
    • , Navin Varadarajan
    • , Aikaterini Kontrogianni-Konstantopoulos
    • , Stuart S. Martin
    •  & Konstantinos Konstantopoulos
  • News & Views |

    Inactivation of the major histocompatibility complex and overexpression of the transmembrane protein CD47 renders induced pluripotent stem cells invisible to the immune system of the host.

    • Tom Shani
    •  & Jacob H. Hanna
  • Perspective |

    This Perspective discusses opportunities and challenges, and proposes a roadmap, for the clinical implementation of optical-imaging biomarkers and technologies for the early detection of cancer.

    • Dale J. Waterhouse
    • , Catherine R. M. Fitzpatrick
    • , Brian W. Pogue
    • , James P. B. O’Connor
    •  & Sarah E. Bohndiek
  • Review Article |

    This Review summarizes the development of optoacoustic imaging with acoustic resolution and wide-bandwidth ultrasound detection, and the applicability of this mesoscopic imaging modality in the label-free visualization of tissue pathophysiology.

    • Murad Omar
    • , Juan Aguirre
    •  & Vasilis Ntziachristos
  • Article |

    An analysis of a proteomics database of leukaemia cell lines and samples from patients with acute myelogenous leukaemia uncovers heterogeneity in protein expression as well as proteomic hallmarks and signatures for patient stratification.

    • C. W. Hu
    • , Y. Qiu
    • , A. Ligeralde
    • , A. Y. Raybon
    • , S. Y. Yoo
    • , K. R. Coombes
    • , A. A. Qutub
    •  & S. M. Kornblau
  • Article |

    A viscoelastic adhesive patch that accommodates the myocardium’s cyclic deformation outperforms most existing acellular epicardial patches in reversing left ventricular remodelling and in restoring heart function after myocardial infarction in rats.

    • Xiao Lin
    • , Yue Liu
    • , Aobing Bai
    • , Huanhuan Cai
    • , Yanjie Bai
    • , Wei Jiang
    • , Huilin Yang
    • , Xinhong Wang
    • , Lei Yang
    • , Ning Sun
    •  & Huajian Gao
  • Article |

    A comparison of the molecular, cellular and functional characteristics of three congruent patient-specific cell types for the modelling of Charcot−Marie−Tooth 1A reveals commonly upregulated chemokines.

    • Bipasha Mukherjee-Clavin
    • , Ruifa Mi
    • , Barbara Kern
    • , In Young Choi
    • , Hotae Lim
    • , Yohan Oh
    • , Benjamin Lannon
    • , Kevin J. Kim
    • , Shaughn Bell
    • , Junho K. Hur
    • , Woochang Hwang
    • , Young Hyun Che
    • , Omer Habib
    • , Robert H. Baloh
    • , Kevin Eggan
    • , Gerald Brandacher
    • , Ahmet Hoke
    • , Lorenz Studer
    • , Yong Jun Kim
    •  & Gabsang Lee
  • Article |

    A thermogelling polymer that acts as an internal tamponade can repair detached retinas and trigger the formation of a vitreous-like body, as shown in retinal-detachment rabbit and non-human-primate models.

    • Zengping Liu
    • , Sing Shy Liow
    • , Siew Li Lai
    • , Asfa Alli-Shaik
    • , Graham E. Holder
    • , Bhav Harshad Parikh
    • , Subramanian Krishnakumar
    • , Zibiao Li
    • , Mein Jin Tan
    • , Jayantha Gunaratne
    • , Veluchamy Amutha Barathi
    • , Walter Hunziker
    • , Rajamani Lakshminarayanan
    • , Clement Woon Teck Tan
    • , Caroline K. Chee
    • , Paul Zhao
    • , Gopal Lingam
    • , Xian Jun Loh
    •  & Xinyi Su
  • News & Views |

    Nanoparticle sensors of the activity of the protease granzyme B detect early T-cell-mediated rejection of transplanted skin grafts in mice via the release of a proteolytically cleaved fluorescence reporter that filters into urine.

    • Anita S. Chong
  • News & Views |

    Depletion of cells expressing the cell-membrane protein programmed cell-death protein-1 using an antibody-based toxin delays the onset of disease in a mouse model of diabetes and reverses paralysis in mice with experimental autoimmune encephalomyelitis, without compromising physiological immunity.

    • Zhirui Wang
    •  & Christene A. Huang
  • News & Views |

    High-throughput nanoparticle synthesis combined with machine learning speeds up the exploration of structure–activity relationships for nanomedicines, as shown for spherical nucleic acids functioning as cancer-vaccine candidates.

    • Yijing Liu
    •  & Xiaoyuan Chen
  • Article |

    A nanomedicine encapsulating a docetaxel prodrug, and conjugated to an antibody specific for the receptor EphA2, provides enhanced antitumour activity in multiple tumour-xenografted mice, and has minimal toxicity in rats and dogs.

    • Walid S. Kamoun
    • , Dmitri B. Kirpotin
    • , Zhaohua Richard Huang
    • , Suresh K. Tipparaju
    • , Charles O. Noble
    • , Mark E. Hayes
    • , Lia Luus
    • , Alexander Koshkaryev
    • , Jaeyeon Kim
    • , Ken Olivier
    • , Tad Kornaga
    • , Shinji Oyama
    • , Vasileios Askoxylakis
    • , Christine Pien
    • , Geoffrey Kuesters
    • , Nancy Dumont
    • , Alexey A. Lugovskoy
    • , Sarah A. Schihl
    • , John H. Wilton
    • , Melissa L. Geddie
    • , James Suchy
    • , Stephanie Grabow
    • , Neeraj Kohli
    • , C. Patrick Reynolds
    • , Rachel Blaydes
    • , Yu Zhou
    • , Andrew J. Sawyer
    • , James D. Marks
    •  & Daryl C. Drummond
  • News & Views |

    An antibody-modified nanoparticle encapsulating a pH-sensitive taxane prodrug, and targeting an overexpressed receptor in tumours, improves the tolerability and anticancer efficacy of the active drug in multiple animal models.

    • Ernesto Moles
    •  & Maria Kavallaris
  • Publisher Correction |

    • Limei Tian
    • , Benjamin Zimmerman
    • , Aadeel Akhtar
    • , Ki Jun Yu
    • , Matthew Moore
    • , Jian Wu
    • , Ryan J. Larsen
    • , Jung Woo Lee
    • , Jinghua Li
    • , Yuhao Liu
    • , Brian Metzger
    • , Subing Qu
    • , Xiaogang Guo
    • , Kyle E. Mathewson
    • , Jonathan A. Fan
    • , Jesse Cornman
    • , Michael Fatina
    • , Zhaoqian Xie
    • , Yinji Ma
    • , Jue Zhang
    • , Yihui Zhang
    • , Florin Dolcos
    • , Monica Fabiani
    • , Gabriele Gratton
    • , Timothy Bretl
    • , Levi J. Hargrove
    • , Paul V. Braun
    • , Yonggang Huang
    •  & John A. Rogers
  • Article |

    A dual positron emission tomography–near-infrared probe enables the visualization of the early trafficking dynamics of an injected model mRNA vaccine in non-human primates.

    • Kevin E. Lindsay
    • , Sushma M. Bhosle
    • , Chiara Zurla
    • , Jared Beyersdorf
    • , Kenneth A. Rogers
    • , Daryll Vanover
    • , Peng Xiao
    • , Mariluz Araínga
    • , Lisa M. Shirreff
    • , Bruno Pitard
    • , Patrick Baumhof
    • , Francois Villinger
    •  & Philip J. Santangelo
  • Article |

    A suite of glass pipettes with integrated microelectrodes that simultaneously acquire intracellular and extracellular data in living mice and rats yield ground-truth data for validation of the performance of spike-sorting algorithms.

    • David L. Hunt
    • , Chongxi Lai
    • , Richard D. Smith
    • , Albert K. Lee
    • , Timothy D. Harris
    •  & Mladen Barbic
  • Article |

    Optoacoustic neuroimaging of mice expressing a genetically encoded calcium indicator allows for rapid, high-resolution, whole-brain 3D snapshots of neuronal calcium activity and haemodynamics.

    • Sven Gottschalk
    • , Oleksiy Degtyaruk
    • , Benedict Mc Larney
    • , Johannes Rebling
    • , Magdalena Anastasia Hutter
    • , Xosé Luís Deán-Ben
    • , Shy Shoham
    •  & Daniel Razansky
  • Article |

    An electrical biosensor combining CRISPR–Cas9 and a graphene field-effect transistor detects target genes in purified genomic samples at high sensitivity, within 15 minutes, and without the need for amplification.

    • Reza Hajian
    • , Sarah Balderston
    • , Thanhtra Tran
    • , Tara deBoer
    • , Jessy Etienne
    • , Mandeep Sandhu
    • , Noreen A. Wauford
    • , Jing-Yi Chung
    • , Jolie Nokes
    • , Mitre Athaiya
    • , Jacobo Paredes
    • , Regis Peytavi
    • , Brett Goldsmith
    • , Niren Murthy
    • , Irina M. Conboy
    •  & Kiana Aran
  • Article |

    The physiological degradation of programmed-death ligand 1 is reduced by the palmitoylation of its intracellular domain, and this process can be inhibited to promote T-cell immunity against tumours.

    • Han Yao
    • , Jiang Lan
    • , Chushu Li
    • , Hubing Shi
    • , Jean-Philippe Brosseau
    • , Huanbin Wang
    • , Haojie Lu
    • , Caiyun Fang
    • , Yao Zhang
    • , Lunxi Liang
    • , Xiaolin Zhou
    • , Chaojun Wang
    • , Yu Xue
    • , Yun Cui
    •  & Jie Xu
  • News & Views |

    Enhanced lysosomal degradation of the transmembrane protein programmed cell-death protein-1 ligand in tumour cells, enabled by blocking the protein’s post-translational lipid modification, promotes T-cell-mediated suppression of tumour growth in mice.

    • Stephane Lefrancois
  • Article |

    A tumour-on-a-chip model featuring patient-derived glioblastoma cells, vascular endothelial cells and decellularized extracellular matrix from brain tissue can be used to identify patient-specific resistance to standard chemoradiotherapy.

    • Hee-Gyeong Yi
    • , Young Hun Jeong
    • , Yona Kim
    • , Yeong-Jin Choi
    • , Hyo Eun Moon
    • , Sung Hye Park
    • , Kyung Shin Kang
    • , Mihyeon Bae
    • , Jinah Jang
    • , Hyewon Youn
    • , Sun Ha Paek
    •  & Dong-Woo Cho
  • News & Views |

    A bioprinted glioblastoma-on-a-chip model enables the evaluation of treatment responses for individual patients whose brain tumours resist standard chemoradiotherapy.

    • Natividad Gomez-Roman
    •  & Anthony J. Chalmers
  • Publisher Correction |

    • Limei Tian
    • , Benjamin Zimmerman
    • , Aadeel Akhtar
    • , Ki Jun Yu
    • , Matthew Moore
    • , Jian Wu
    • , Ryan J. Larsen
    • , Jung Woo Lee
    • , Jinghua Li
    • , Yuhao Liu
    • , Brian Metzger
    • , Subing Qu
    • , Xiaogang Guo
    • , Kyle E. Mathewson
    • , Jonathan A. Fan
    • , Jesse Cornman
    • , Michael Fatina
    • , Zhaoqian Xie
    • , Yinji Ma
    • , Jue Zhang
    • , Yihui Zhang
    • , Florin Dolcos
    • , Monica Fabiani
    • , Gabriele Gratton
    • , Timothy Bretl
    • , Levi J. Hargrove
    • , Paul V. Braun
    • , Yonggang Huang
    •  & John A. Rogers
  • Article |

    A microphysiological model of the bronchial airways enables the study of the mechanochemical feedback interactions between smooth muscle cells and epithelial cells that underlie bronchospasm.

    • Onur Kilic
    • , Arum Yoon
    • , Sagar R. Shah
    • , Hwan Mee Yong
    • , Alejandro Ruiz-Valls
    • , Hao Chang
    • , Reynold A. Panettieri Jr
    • , Stephen B. Liggett
    • , Alfredo Quiñones-Hinojosa
    • , Steven S. An
    •  & Andre Levchenko
  • Editorial |

    Peer review relies on organized scepticism and implicit trust. A healthy balance of both qualities should also prevail when peer review fails.

  • News & Views |

    Large-area electrode arrays for epidermal electrophysiology offer new possibilities for the control of prosthetic devices and the monitoring of brain function.

    • Damiano G. Barone
    •  & George G. Malliaras
  • News & Views |

    Accurate and explainable detection, via deep learning, of acute intracranial haemorrhage from computed tomography images of the head is achievable with small amounts of data for model training.

    • Michael C. Muelly
    •  & Lily Peng
  • News & Views |

    A patch that deposits an array of microneedle tips under the top layer of the skin enables weeks-long delivery of a contraceptive in rats.

    • Ryan F. Donnelly
    •  & Eneko Larraňeta
  • News & Views |

    An inexpensive assay that uses thermophoresis to concentrate cancer extracellular vesicles bound to aptamers specific for target proteins on the vesicles’ surface, can classify cancer patients according to cancer type and stage.

    • Hakho Lee
    •  & Cesar M. Castro
  • News & Views |

    By compressing blood vessels and neurons, globular tumours in the brain can cause neuronal damage, which can be partially reversed by relieving the stress via tumour removal or by administering lithium.

    • Paul A. Janmey
    •  & Katarzyna Pogoda
  • News & Views |

    A computationally designed small molecule inhibits the replication of prion-protein aggregates and ameliorates neurological symptoms in mouse and non-human-primate models of transmissible spongiform encephalopathy.

    • Emiliano Biasini
  • Article |

    Deep learning can be used to virtually stain autofluorescence images of unlabelled tissue sections, generating images that are equivalent to the histologically stained versions.

    • Yair Rivenson
    • , Hongda Wang
    • , Zhensong Wei
    • , Kevin de Haan
    • , Yibo Zhang
    • , Yichen Wu
    • , Harun Günaydın
    • , Jonathan E. Zuckerman
    • , Thomas Chong
    • , Anthony E. Sisk
    • , Lindsey M. Westbrook
    • , W. Dean Wallace
    •  & Aydogan Ozcan
  • Article |

    An immunotoxin incorporating an anti-programmed-cell-death-protein-1 (PD-1) single-chain variable fragment selectively eliminates PD-1-expressing cells, and suppresses autoimmunity while preserving adaptive immunity in mouse models of autoimmune disease.

    • Peng Zhao
    • , Peng Wang
    • , Shuyun Dong
    • , Zemin Zhou
    • , Yanguang Cao
    • , Hideo Yagita
    • , Xiao He
    • , Song Guo Zheng
    • , Simon J. Fisher
    • , Robert S. Fujinami
    •  & Mingnan Chen
  • Article |

    A mechanical injector that targets tissue by sensing the loss-of-resistance on encountering softer tissue or a cavity can reliably and precisely deliver liquids to the suprachoroidal space, and to other injection-accessible target sites in the body.

    • Girish D. Chitnis
    • , Mohan K. S. Verma
    • , Julien Lamazouade
    • , Miguel Gonzalez-Andrades
    • , Kisuk Yang
    • , Ali Dergham
    • , Peter Anthony Jones
    • , Benjamin E. Mead
    • , Andrea Cruzat
    • , Zhixiang Tong
    • , Keir Martyn
    • , Aniruddh Solanki
    • , Natalie Landon-Brace
    •  & Jeffrey M. Karp
  • Article |

    Intravenous delivery of an adenine base editor and a single-guide RNA for the Fah gene can correct an A>G splice-site mutation in an adult mouse model of tyrosinaemia.

    • Chun-Qing Song
    • , Tingting Jiang
    • , Michelle Richter
    • , Luke H. Rhym
    • , Luke W. Koblan
    • , Maria Paz Zafra
    • , Emma M. Schatoff
    • , Jordan L. Doman
    • , Yueying Cao
    • , Lukas E. Dow
    • , Lihua Julie Zhu
    • , Daniel G Anderson
    • , David R. Liu
    • , Hao Yin
    •  & Wen Xue