Volume 27

  • No. 7 July 2022

    Single molecule fluorescentin situ hybridisation (smFISH) images of WT (left) and NLGF (right) CA1 hippocampal region. smFISH labels mRNA of Fzd1 (green), Fzd7 (yellow) and Rbfox3 (magenta) and DAPI labels nuclei (blue) show a decreased levels of expression of Fzd1 and Fzd7. For more information see the article by Palomer et al. on pages 3024-3033.

  • No. 6 June 2022

    Stellate cells from the entorhinal cortex (labelled for reelin in green) project to VIP-expressing neurons in the hippocampal CA2 area (presynaptic neurons are labelled with mCherry using pseudotyped rabies virus injection in the CA2 of VIP-Cre mice. For more information see the article by Leroy et al. on pages 2879-2900.

  • No. 5 May 2022

    Image of the dorsal raphe nucleus stained with 5-HT, DAPI and IL1R1 shRNA expressing GFP cells. For more information see the article by Takahashi et al. on pages 2563-2579.

  • No. 4 April 2022

    Trisomy 21 (T21) causes Down syndrome and an early-onset form of Alzheimer’s disease (AD). In this issue, Wu et al utilized human induced pluripotent stem cells (hiPSCs) along with CRISPR-Cas9 gene editing to investigate the contribution of chromosome 21 candidate genes to AD-relevant neuronal phenotypes. Shown are representative immunostaining images of neurons derived from a T21 iPSC line (bottom panels) and a T21 iPSC line which lost one copy of HSA21 (top panels). IPSC-derived neurons are immunostained for bIII-tubulin (green, left panels), neurofilament heavy chain (NEFH, red, middle panels) and POU Class 3 Homeobox 2 (POU3F2, pink, right panels). Nuclei are stained with DAPI in all images. For more information, see pages 1970-1989. Images acquired by Elizabeth Vinton.

  • No. 3 March 2022

    Increased neural stem cell/progenitor apoptosis in Schizophrenia patient-derived 3D cerebral organoids. Shown are two whole-organoid images generated from a healthy adult iPSC donor (left) and from a Schizophrenia patient iPSC donor (right). Depicted is the colocalization of early SOX2+ (green) neural stem cells/progenitors and the apoptotic marker cleaved Caspase-3 (red). Cells are counterstained with DAPI (blue). We detected a substantial increase in the rate of progenitor death in Schizophrenia patient-derived cerebral organoids relative to rates observed in organoids generated from healthy control iPSC donors. In the manuscript by Notaras et al., it is shown that Pleiotrophin (PTN) but not BRN2 (POU3F2) could rescue premature progenitor death in Schizophrenia patient-derived cerebral organoids. For more information see the article by Notaras et al. on pages 1416–1434.

  • No. 2 February 2022

    Human neurons with 1q21.1 deletion or duplication are associated with developmental deficits. Cortical preplate marker T-Box Brain 1 (TBR1, green, upper panel) and deep layer marker Coup-TFI Interacting Protein 2 (CTIP2, green, lower panel) were differentially expressed in iPSC derived MAP2+ neurons (red) carrying 1q21.1 deletion (centre) or duplication (right) in comparison to non-carrier controls (left). The nuclei are stained with DAPI (blue)". For more information see the article by Chapman et al. on pages 819-830.

  • No. 1 January 2022

    Image showing induced pluripotent stem cells-derived excitatory and inhibitory neurons. For more information see the article by Mossink et al. on pages 1-18.