Volume 26

  • No. 5 May 2021

    Immunofluorescent staining of neuron-microglia co-cultures. Images show neurons from Pten WT/WT and from Pten autism mouse model Pten WT/m3m4 , and Pten m3m4/m3m (Left to right) co-cultured with Pten WT/WT microglia (Top panels) and Pten m3m4/m3m microglia (Bottom panels). Neurons are labeled green with Tuj1; C1q (red) punctae show accumulation on neuronal surfaces. For more information see the article by Prof. Charis Eng et al. on page 1458-1471.

  • No. 4 April 2021

    Expression of the immediate early gene Arc (red) in mature (green) and newborn (green and blue) dentate granule neurons in mice with chronic DSS colitis during acquisition of spatial learning. For more information see the article by Katia P Karalis et al. on page 1248-1263.

  • No. 3 March 2021

    Immunostaining of the neuronal marker beta III tubulin (green) and the transcription factor nuclear factor kappa B p65 (NF-κB, also known as RelA, red) in the LUHMES human neuronal cell line at differentiation day 2 (upper panels) and day 6 (lower panels). Nuclei are stained with Hoechst (blue). Inactive NF-κB is held in the cytoplasm by its inhibitor, IκB (left panels). Upon activation, IκB is phosphorylated by IκB kinase (IKK), and NF-κB is able to translocate into the nucleus, resulting in a pink color in LUHMES cells exposed to a mixture of inflammatory cytokines (middle panels). Pretreatment with the IKK inhibitor TPCA-1 largely prevents NF-κB nuclear translocation following cytokine exposure (right panels). For more information see the article by Prof. Pamela Lein et al. on pages 875-887.

  • No. 2 February 2021

    Cell-specific increased surface P2X4mCherryIN in P2X4 internalization-defective knock-in mice. Surface P2X4 (green) is almost absent in MAP2-positive hippocampal neurons (magenta) of control mice expressing wild-type P2X4 but highly increased at the surface of CaMK2Cre-P2X4mCherryIN mice expressing non-internalized P2X4mCherryIN in excitatory neurons (first, second column). Middle, enlarged image of a neuronal dendrite, showing intracellular (red) and surface clustering of P2X4mCherryIN (green) at extra- and post-synaptic regions indicated by Homer 1c (blue). Right column, P2X4mCherryIN (red) is expressed in GFAP-positive (green) astrocytes from hippocampal cultures of CMVCre-P2X4mCherryIN mice. For more information see the article by Eléonore Bertin et al. on pages 629-644.

  • No. 1 January 2021

    Single molecule fluorescence in situ hybridization showing Mineralocorticoid Receptor (Nr3c2) expression in mouse hippocampal area CA2. Left: Nr3c2 expression (magenta) in CA1 (top inset) compared with CA2 (bottom inset). Right: Same as left but also including nuclei (blue) and CA2 markers Pcp4 (green) and Acan (red). For more information see the article by Dr. Serena Dudek et al. on pages 350-364.