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Immunofluorescent staining of neuron-microglia co-cultures. Images show neurons from PtenWT/WT and from Pten autism mouse model PtenWT/m3m4, and Ptenm3m4/m3m (Left to right) co-cultured with PtenWT/WT microglia (Top panels) and Ptenm3m4/m3m microglia (Bottom panels). Neurons are labeled green with Tuj1; C1q (red) punctae show accumulation on neuronal surfaces. For more information see the article by Prof. Charis Eng et al. on page 1458-1471.