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Immunofluorescent labeling of surface (red) and intracellular (green) HA-neuroligin-3 protein in HeLa cells (untreated; top panels) and following the addition of PMA (200 nM for 30 minutes; bottom panel). The activation of PKC dramatically reduces surface neuroligin-3 levels. DAPI was used to label cell nuclei (blue).
For more information, please refer to the paper by Bemben et al. on pages 145–160.